Living Radical Polymerization as a Tool for the Synthesis of Polymer‐Protein/Peptide Bioconjugates

Nicolas, Julien; Mantovani, Giuseppe; Haddleton, David M.

doi:10.1002/marc.200700112

Cited by 314 publications

(277 citation statements)

References 249 publications

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“…This is the most commonly exploited approach to make protein and other biopolymer conjugates. [491][492][493][494] Note that use of functionality on 'Z' leaves the thiocarbonylthio group as a potentially degradable link at the block juncture. This may or may not be advantageous depending on the application.…”

Section: Block Copolymersmentioning

confidence: 99%

Living Radical Polymerization by the RAFT Process - A Second Update

Moad¹,

Rizzardo²,

Thang³

2009

Aust. J. Chem.

902

720

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This paper provides a second update to the review of reversible deactivation radical polymerization achieved with thiocarbonylthio compounds (ZC(=S)SR) by a mechanism of reversible addition-fragmentation chain transfer (RAFT) that was published in June 2005 (Aust. J. Chem. 2005, 58, 379-410). The first update was published in November 2006 (Aust. J. Chem. 2006, 59, 669-692). This review cites over 500 papers that appeared during the period mid-2006 to mid-2009 covering various aspects of RAFT polymerization ranging from reagent synthesis and properties, kinetics and mechanism of polymerization, novel polymer syntheses and a diverse range of applications. Significant developments have occurred, particularly in the areas of novel RAFT agents, techniques for end-group removal and transformation, the production of micro/nanoparticles and modified surfaces, and biopolymer conjugates both for therapeutic and diagnostic applications.

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Section: Block Copolymersmentioning

confidence: 99%

Living Radical Polymerization by the RAFT Process - A Second Update

Moad¹,

Rizzardo²,

Thang³

2009

Aust. J. Chem.

902

720

View full text Add to dashboard Cite

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“…Convergent methodologies require a 80 conjugation step, where both building blocks are synthesised initially and then coupling together via ligation. Divergent approaches on the other hand require either the modification of the peptide or polymer block (to subsequently allow "growth" of the other component from first moiety) or the 85 conversion of the peptidic component into a macromonomer for subsequent copolymerisation. This latter approach, also known as "grafting through", is often not referred to as a divergent approach as the peptide sequence has already been defined prior to incorporation along the polymer backbone.…”

Section: Synthetic Approachesmentioning

confidence: 99%

“…70 Whilst useful in determining the phase space for gelation, these methods cannot be used to accurately measure the mechanical properties of the gel. Nonetheless, these methods are the most highly reported in the literature for the demonstration of 85 the formation of a gel. As a result, direct comparison between different systems is often extremely difficult.…”

mentioning

confidence: 99%

Peptide conjugate hydrogelators

2010

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5Molecular gelators are currently receiving a great deal of attention. These are small molecules which, under the appropriate conditions, assemble in solution to, in the majority of cases, give long fibrillar structures which entangle to form a three-dimensional network. This immobilises the solvent, resulting in a gel. Such gelators have potential application in a number of important areas from drug delivery to tissue engineering. Recently, the use of peptide-conjugates has become 10 prevalent with oligopeptides (from as short as two amino acids in length) co njugated to a polymer, alkyl chain or aromatic group such as naphthalene or fluorenylmethoxycarbonyl (Fmoc) being shown to be effective molecular gelators. The field of gelation is extremely large; here we will focus our attention on the use of these peptide-conjugates as molecular hydrogelators.

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“…Ten et al reported the solid phase supported synthesis routes to obtain the oligopeptide-based RAFT agents. 20 The peptide-polymer hybrid materials were synthesized by the ATRP from resin-supported peptides by Washburn et al, 21 and the peptide-polymer bioconjugate block copolymers through the nitroxide-mediated radical polymerization (NMRP) from a solid support was shown by Wooley group. 12 In this study, we described our strategy to prepare the peptide-polymer hybrid materials by combining ATRP method with solid phase peptide synthesis.…”

Section: Introductionmentioning

confidence: 99%

Solid Phase Synthesis of Lysine-exposed Peptide-Polymer Hybrids by Atom Transfer Radical Polymerization

Ha¹,

Kim²,

Kim³

et al. 2014

Polymer Korea

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Recently, the peptide(or protein)-polymer hybrid materials (PPs) were sought in many research areas as potential building blocks for assembling nanostructures in selective solvents. In PPs, the facile routes of preparing well-defined peptide-polymer bio-conjugates and their specific activities in various applications are important issues. Our strategy to prepare the peptide-polymer hybrid materials was to combine atom transfer radical polymerization (ATRP) method with solid phase peptide synthesis. The standard solid phase peptide synthesis method was employed to prepare the PYGK (proline-tyrosine-glycine-lysine) peptide. PYGK is an analogue peptide, PFGK (proline-phenylalanine-glycine-lysine), which interacted with plasminogen in fibrinolysis. The peptide and the peptide-initiator were characterized with MALDI-TOF mass spectrometry and 1 H NMR spectrometer. The peptide-polymer, pSt-PYGK was characterized by GPC, IR, 1 H NMR spectrometer and TLC. Spherical micellar aggregates were determined by TEM and SEM. Current synthesis methodology suggested opportunities to create the well-defined peptide-polymer hybrid materials with specific binding activity.

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Living Radical Polymerization as a Tool for the Synthesis of Polymer‐Protein/Peptide Bioconjugates

Cited by 314 publications

References 249 publications

Living Radical Polymerization by the RAFT Process - A Second Update

Living Radical Polymerization by the RAFT Process - A Second Update

Peptide conjugate hydrogelators

Solid Phase Synthesis of Lysine-exposed Peptide-Polymer Hybrids by Atom Transfer Radical Polymerization

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