Living on the edge: biofilms developing in oscillating environmental conditions

Dobretsov, Sergey; Abed, Raeid M. M.; Muthukrishnan, T. S.; Sathe, Priyanka; Al-Naamani, Laila; Queste, Bastien Y.; Piontkovski, Sergey

doi:10.1080/08927014.2018.1539707

Cited by 13 publications

(27 citation statements)

References 55 publications

(59 reference statements)

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“…In contrast, chitosan paints significantly reduced densities of micro-fouling on the surface of a sea glider exposed to periodical oscillations of environmental conditions in the Sea of Oman. Moreover, it was found that the structure of microbial communities formed on chitosan paints were similar to unprotected surfaces and were totally different from what is found on surfaces coated with copper-based antifouling paints, suggesting that chitosan-based coatings have minimal negative impact on marine communities [131].…”

Section: Antifouling Paints and Coatingsmentioning

confidence: 88%

Chitosan Nanocomposite Coatings for Food, Paints, and Water Treatment Applications

et al. 2019

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Worldwide, millions of tons of crustaceans are produced every year and consumed as protein-rich seafood. However, the shells of the crustaceans and other non-edible parts constituting about half of the body mass are usually discarded as waste. These discarded crustacean shells are a prominent source of polysaccharide (chitin) and protein. Chitosan is a de-acetylated form of chitin obtained from the crustacean waste that has attracted attention for applications in food, biomedical, and paint industries due to its characteristic properties, like solubility in weak acids, film-forming ability, pH-sensitivity, biodegradability, and biocompatibility. We present an overview of the application of chitosan in composite coatings for applications in food, paint, and water treatment. In the context of food industries, the main focus is on fabrication and application of chitosan-based composite films and coatings for prolonging the post-harvest life of fruits and vegetables, whereas anti-corrosion and self-healing properties are the main properties considered for antifouling applications in paints in this review.

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Section: Antifouling Paints and Coatingsmentioning

confidence: 88%

Chitosan Nanocomposite Coatings for Food, Paints, and Water Treatment Applications

et al. 2019

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“…We observed a similar taxonomic shift with a progressive increase of Proteobacteria abundance (particularly Gammaproteobacteria). Marine biofilm communities are selected from planktonic communities but appear to be dissimilar from them (Lee et al, 2008;Briand et al, 2012;Dobretsov et al, 2018). These differences could also be due to the distinct structures between the complex and organized cellular of biofilms and of individual, planktonic cells which are present in the water column (Doghri et al, 2015).…”

Section: Taxonomy Of Biofilms On Plates With Anti-corrosion Paint Andmentioning

confidence: 99%

Distinct Temporal Succession of Bacterial Communities in Early Marine Biofilms in a Portuguese Atlantic Port

et al. 2020

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Marine biofilms are known to influence the corrosion of metal surfaces in the marine environment. Despite some recent research, the succession of bacterial communities colonizing artificial surfaces remains uncharacterized in some temporal settings. More specifically, it is not fully known if bacterial colonizers of artificial surfaces are similar or distinct in the different seasons of the year. In particular the study of early biofilms, in which the bacterial cells communities first adhere to artificial surfaces, are crucial for the development of the subsequent biofilm communities. In this work, we used amplicon-based NGS (next-generation sequencing) and universal 16S rRNA bacterial primers to characterize the early biofilm bacterial communities growing on 316 L stainless steel surfaces in a Northern Portugal port. Sampling spanned 30-day periods in two distinct seasons (spring and winter). Biofilm communities growing in steel surfaces covered with an anti-corrosion paint and planktonic communities from the same location were also characterized. Our results demonstrated that distinct temporal patterns were observed in the sampled seasons. Specifically, a significantly higher abundance of Gammaproteobacteria and Mollicutes was found on the first days of biofilm growth in spring (day 1 to day 4) and a higher abundance of Alphaproteobacteria during the same days of biofilm growth in winter. In the last sampled day (day 30), the spring biofilms significantly shifted toward a dominance of photoautotrophic groups (mostly diatoms) and were also colonized by some macrofouling communities, something not observed during the winter sampling. Our results revealed that bacterial composition in the biofilms was particularly affected by the sampled day of the specific season, more so than the overall effect of the season or overall sampling day of both seasons. Additionally, the application of a non-fouling-release anti-corrosion paint in the steel plates resulted in a significantly lower diversity compared with plates without paint, but this was only observed during spring. We suggest that temporal succession of marine biofilm communities should be taken in consideration for future antifouling/anti-biofilm applications.

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“…Planctomycetes (classes of BD7-11 and OM190) that were found abundant in the biofilms sampled from all three surface types (>2.5%) in this study, have also been previously recorded on fouling control surfaces (Leary et al ., 2014; von Ammon et al ., 2018; Ding et al ., 2019), although this phylum is underestimated by previous NGS biofilm studies on fouling control coatings (e.g. Muthukrishnan et al ., 2014; Briand et al ., 2017; Flach et al ., 2017; Hunsucker et al ., 2018; Winfield et al ., 2018; Dobretsov et al ., 2019). Although not frequently reported, Verrucomicrobia has also been found in fouling control studies (Leary et al ., 2014; Winfield et al ., 2018), and was confirmed to be abundant in all three coating treatments (>1.8%) in this study.…”

Section: Discussionmentioning

confidence: 99%

“…To date, only a handful of studies have reported the application of next generation sequencing (NGS) techniques to investigate the response of marine biofilm community profiles developed on marine fouling control coatings (Muthukrishnan et al ., 2014; Leary et al ., 2014; Briand et al ., 2017; Flach et al ., 2017; Hunsucker et al ., 2018; Winfield et al ., 2018; von Ammon et al ., 2018; Dobretsov et al ., 2019; Ding et al ., 2019). NGS technology based on sequencing ribosomal RNA genes, is appropriate for a range of applications including highly diverse community analysis, while offering large volume of data that allow for statistical testing (Fukuda et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

“…NGS technology based on sequencing ribosomal RNA genes, is appropriate for a range of applications including highly diverse community analysis, while offering large volume of data that allow for statistical testing (Fukuda et al ., 2016). All studies employing high-throughput NGS have reported the dominance of Alphaproteobacteria on fouling control coatings, whilst Gammaproteobacteria have also been identified as key players in fouling control systems (Muthukrishnan et al ., 2014; Leary et al ., 2014; Briand et al ., 2017; Flach et al ., 2017; Hunsucker et al ., 2018; von Ammon et al ., 2018; Dobretsov et al ., 2019; Ding et al ., 2019). Biofilms on fouling control coatings have also been dominated by Flavobacteria (Muthukrishnan et al ., 2014; Leary et al ., 2014; Hunsucker et al ., 2018; von Ammon et al ., 2018) or Cyanobacteria (Muthukrishnan et al ., 2014; Leary et al ., 2014; Hunsucker et al ., 2018; Ding et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

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Marine biofilms on different fouling control coating types reveal differences in microbial community composition and abundance

Papadatou

Robson

Dobretsov

et al. 2021

Preprint

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Marine biofouling imposes serious environmental and economic impacts on marine applications, especially in the shipping industry. To combat biofouling, protective coatings are applied on vessel hulls which are divided into two major groups: biocidal and non-toxic fouling-release. The aim of the current study was to explore the effect of coating type on microbial biofilm community profiles to better understand the differences between the communities developed on fouling control biocidal antifouling and biocidal-free coatings. Biocidal (Intersmooth(R) 7460HS SPC), fouling-release (Intersleek(R) 900), and inert surfaces were deployed in the marine environment for 4 months and the biofilms that developed on these surfaces were investigated using Illumina NGS sequencing, targeting the prokaryotic 16S rRNA gene. The results confirmed differences in the community profiles between coating types. The biocidal coating supported communities dominated by Alphaproteobacteria (Loktanella, Sphingorhabdus, Erythrobacter) and Bacteroidetes (Gilvibacter), whilst other taxa such as Portibacter and Sva0996 marine group, proliferated on the fouling-release surface. Knowledge of these marine biofilm components on fouling control coatings will serve as a guide for future investigations of marine microfouling as well as informing the coatings industry of potential microbial targets for robust coating formulations.

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Living on the edge: biofilms developing in oscillating environmental conditions

Cited by 13 publications

References 55 publications

Chitosan Nanocomposite Coatings for Food, Paints, and Water Treatment Applications

Chitosan Nanocomposite Coatings for Food, Paints, and Water Treatment Applications

Distinct Temporal Succession of Bacterial Communities in Early Marine Biofilms in a Portuguese Atlantic Port

Marine biofilms on different fouling control coating types reveal differences in microbial community composition and abundance

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