Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion

Aknoun, Shérazade; Savatier, Julien; Bon, Pierre; Galland, Frédéric; Abdeladim, Lamiae; Wattellier, Benoît; Monneret, Serge

doi:10.1117/1.jbo.20.12.126009

Cited by 89 publications

(93 citation statements)

References 35 publications

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“…Previous reported results are consecutive measurements of the same cells estimating repeatability term only, which cannot be translated into precision. Reproducibility measurement on red blood cells using LSI is reported in [9]. Although these objects differ significantly from the adherent mammalian cells measured in this study, the reported precision of 0.8 µm 3 is comparable to our results.…”

Section: Estimation Of the Precision Of The Cell Ovd Measurementssupporting

confidence: 82%

“…(8) is well posed and the standard deviations σ i = √ V i , the instruments precision, can be estimated via Eq. (9). The results of these calculations are given in Table 3.…”

Section: Estimation Of the Precision Of The Cell Ovd Measurementsmentioning

confidence: 99%

“…The OPD values can be integrated over the total projected area S of the cell. Here we use the optical volume difference (OVD) denomination introduced in [9] to define this integral. It is expressed as a unit of volume in µm 3 :…”

Section: Introductionmentioning

confidence: 99%

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Quantitative phase imaging of adherent mammalian cells: a comparative study

Allier¹,

Hervé²,

Mandula³

et al. 2019

Biomed. Opt. Express

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The quantitative phase imaging methods have several advantages when it comes to monitoring cultures of adherent mammalian cells. Because of low photo-toxicity and no need for staining, we can follow cells in a minimally invasive way over a long period of time. The ability to measure the optical path difference in a quantitative manner allows the measurement of the cell dry mass, an important metric for studying the growth kinetics of mammalian cells. Here we present and compare cell measurements obtained with three different techniques: digital holographic microscopy, lens-free microscopy and quadriwave lateral sheering interferometry. We report a linear relationship between optical volume density values measured with these different techniques and estimate the precisions of this measurement for the different individual instruments.

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Section: Estimation Of the Precision Of The Cell Ovd Measurementssupporting

confidence: 82%

“…(8) is well posed and the standard deviations σ i = √ V i , the instruments precision, can be estimated via Eq. (9). The results of these calculations are given in Table 3.…”

Section: Estimation Of the Precision Of The Cell Ovd Measurementsmentioning

confidence: 99%

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Quantitative phase imaging of adherent mammalian cells: a comparative study

Allier¹,

Hervé²,

Mandula³

et al. 2019

Biomed. Opt. Express

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“…We note that the effects of PFA fixation on cell mass and Raman spectra were found to be not significant , 29,30 so that the results shown here should be comparable to experiments on live cells. Our work thus paves way for live cell imaging with CARS microscopy to measure the dynamics of cell components in various bioprocesses/metabolism.…”

Section: Discussionsupporting

confidence: 66%

Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Karuna

Masia

Wiltshire

et al. 2019

Multiphoton Microscopy in the Biomedical Sciences XIX

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Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering," ABSTRACT Quantitative determination of the chemical composition of unstained samples, non-invasively, with high threedimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.

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“…Quantitative phase imaging (QPI) has been shown to be very well suited for live cell imaging (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32). QPI provides high contrast images free of the optical artifacts associated with phase contrast images, allowing basic and well established segmentation algorithms to be used.…”

Section: Introductionmentioning

confidence: 99%

Label‐free high temporal resolution assessment of cell proliferation using digital holographic microscopy

Janicke¹,

Kårsnäs²,

Egelberg³

et al. 2017

Cytometry Pt A

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Cell proliferation assays are widely applied in biological sciences to understand the effect of drugs over time. However, current methods often assess cell population growth indirectly, that is, the cells are not actually counted. Instead other parameters, for example, the amount of protein, are determined. These methods often also demand phototoxic labels, have low temporal resolution, or employ end-point assays, and frequently are labor intensive. We have developed a robust and label-free kinetic cell proliferation assay with high temporal resolution for adherent cells using digital holographic microscopy (DHM), one of many quantitative phase microscopy techniques. As no labels or stains are required, and only very low intensity illumination is necessary, the technique allows for noninvasive continuous cell counting. Only two image processing settings were adjusted between cell lines, making the assay practical, user friendly, and free of user bias. The developed direct assay was validated by analyzing cell cultures treated with various concentrations of the anti-cancer drug etoposide, a well-established topoisomerase inhibitor that causes DNA damage and leads to programmed cell death. After treatment, the unstained adherent cells were nondestructively imaged every 30 min for 36 h inside a cell incubator. In the recorded time-lapse image sequences, individual cells were automatically identified to provide detailed growth curves and growth rate data of cell number, confluence, and average cell volume. Our results demonstrate how these parameters facilitate a deeper understanding of cell processes than what is achievable with current single-parameter and end-point methods. © 2017 International Society for Advancement of Cytometry.

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Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion

Cited by 89 publications

References 35 publications

Quantitative phase imaging of adherent mammalian cells: a comparative study

Quantitative phase imaging of adherent mammalian cells: a comparative study

Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Label‐free high temporal resolution assessment of cell proliferation using digital holographic microscopy

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