Livestock-associated methicillin-resistant Staphylococcus aureus in Czech retailed ready-to-eat meat products

Gelbíčová, Tereza; Brodíková, Kristýna; Karpíšková, Renata

doi:10.1016/j.ijfoodmicro.2022.109727

Cited by 12 publications

(8 citation statements)

References 35 publications

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“…several authors [24,25], raw materials or ingredients are one of the main sources of S. aureus contamination in meat processing plants, data in agreement with our results on higher contamination in batters and casings. In our work, MRSA presence was relatively high (13.2%), although the pathogen was detected only in one of the industries investigated.…”

Section: Discussionsupporting

confidence: 93%

Staphylococcus aureus in the Processing Environment of Cured Meat Products

Pérez-Boto¹,

D’Arrigo²,

García–Lafuente³

et al. 2023

Preprint

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The presence of Staphylococcus aureus in six dry-cured meat-processing facilities was investigated. S. aureus was detected in 3.8% of surfaces from five facilities. Prevalence was clearly higher during processing (4.8%) than after cleaning and disinfection (1.4%). Thirty eight isolates were typified by PFGE and MLST. Eleven sequence types (STs) were defined by MLST. ST30 (32%) and ST12 (24%), were the most abundant. Enterotoxin genes were detected in 53% of isolates. The enterotoxin A gene (sea) was present in all ST30 isolates, seb in one ST1 isolate and sec in two ST45 isolates. Sixteen isolates harbored the enterotoxin gene cluster (egc) with four variations in the sequence. The toxic shock syndrome toxin gene (tst) was detected in 82% of isolates. Regarding the antimicrobial resistance, twelve strains were susceptible to all the antibiotics tested (31.6%). However, 15.8% were resistant to three or more antimicrobials, and therefore multidrug-resistant. Our results showed that, in general, efficient cleaning and disinfection procedures were applied. Nonetheless, the presence of S. aureus with virulence determinants and resistance to antimicrobials, and particularly multidrug resistant MRSA ST398 strains might represent a potential health hazard for consumers, even though the pathogen was not isolated from final products.

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Section: Discussionsupporting

confidence: 93%

Staphylococcus aureus in the Processing Environment of Cured Meat Products

Pérez-Boto¹,

D’Arrigo²,

García–Lafuente³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The incidence reported by Gounadaki et al [23] in food contact and non-contact surfaces in three of seven processing plants producing traditional fermented and/or dry sausages was 11.7%, whereas the pathogen was not detected in batter or final products. According to several authors [24,25], raw materials or ingredients are one of the main sources of S. aureus contamination in meat processing plants; these data agree with our results on higher contamination in batters and casings. In our study, MRSA presence was relatively high (10.5%), although the pathogen was detected only in one of the industries investigated.…”

Section: Discussionsupporting

confidence: 93%

Staphylococcus aureus in the Processing Environment of Cured Meat Products

Pérez-Boto

D’Arrigo

García–Lafuente

et al. 2023

Foods

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The presence of Staphylococcus aureus in six dry-cured meat-processing facilities was investigated. S. aureus was detected in 3.8% of surfaces from five facilities. The occurrence was clearly higher during processing (4.8%) than after cleaning and disinfection (1.4%). Thirty-eight isolates were typified by PFGE and MLST. Eleven sequence types (STs) were defined by MLST. ST30 (32%) and ST12 (24%) were the most abundant. Enterotoxin genes were detected in 53% of isolates. The enterotoxin A gene (sea) was present in all ST30 isolates, seb in one ST1 isolate, and sec in two ST45 isolates. Sixteen isolates harbored the enterotoxin gene cluster (egc) with four variations in the sequence. The toxic shock syndrome toxin gene (tst) was detected in 82% of isolates. Regarding antimicrobial resistance, twelve strains were susceptible to all the antibiotics tested (31.6%). However, 15.8% were resistant to three or more antimicrobials and, therefore, multidrug-resistant. Our results showed that in general, efficient cleaning and disinfection procedures were applied. Nonetheless, the presence of S. aureus with virulence determinants and resistance to antimicrobials, particularly multidrug-resistant MRSA ST398 strains, might represent a potential health hazard for consumers.

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“…The prevalence of its nasal carriage in pigs and contamination of pig slaughter batches can be very high [ 8 , 9 ]. In pigs, LA-MRSA CC398 is of limited clinical importance for animals but poses a risk of zoonotic transmission not only to people in close contact with livestock or dust in intensive animal housings, but also to the community through the food chain [ 10 , 11 , 12 ]. LA-MRSA has also been introduced into the healthcare settings in areas with high density of livestock farming [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus from Two Pig Farms: Longitudinal Study of LA-MRSA

et al. 2022

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Pigs were identified as the most important reservoir of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA), mostly belonging to the emergent zoonotic clonal complex (CC) 398. Here, we investigated the presence of MRSA in sows and piglets over a period of several months in two pig farms (intensive farm A and family-run farm B). Isolates underwent antimicrobial susceptibility testing, PCR characterization and spa typing. We collected 280 samples, namely 206 nasal swabs from pigs and 74 environmental samples from pig housings at 12 consecutive time points. A total of 120/161 (74.5%) and 75/119 (63.0%) samples were MRSA-positive in farms A and B, respectively. All isolates harbored mecA but lacked mecC and PVL-encoding genes. The identified spa types (t571, t034, t1250 and t898 in farm A, t1451 and t011 in farm B) were indicative of CC398. Antimicrobial resistance patterns (all multidrug resistant in farm A, 57.2% in farm B) depended on the farm, suggesting the impact of farm size and management practices on the prevalence and characteristics of MRSA. Due to the intermittent colonization of pigs and the high contamination of their immediate environment, MRSA status should be determined at the farm level when considering preventive measures or animal trade between farms.

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Livestock-associated methicillin-resistant Staphylococcus aureus in Czech retailed ready-to-eat meat products

Cited by 12 publications

References 35 publications

Staphylococcus aureus in the Processing Environment of Cured Meat Products

Staphylococcus aureus in the Processing Environment of Cured Meat Products

Staphylococcus aureus in the Processing Environment of Cured Meat Products

Antimicrobial Resistance and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus from Two Pig Farms: Longitudinal Study of LA-MRSA

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