Lives of a Heart Cell: Tracing the Origins of Cardiac Progenitors

Martı́n-Puig, Silvia; Wang, Zhong; Chien, Kenneth R.

doi:10.1016/j.stem.2008.03.010

Cited by 182 publications

(140 citation statements)

References 97 publications

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“…cells can be converted in vitro into CMs when cocultured with rCMs, but they cannot be converted in vivo Our working hypothesis was that an absent or insufficient blood perfusion and an altered ECM might be reinto CMs (29), despite a supposedly favorable microenvironment that favors their phenotypic conversion into sponsible for a suboptimal engraftment and cardiovascular differentiation of transplanted stem/progenitor cells endothelial and smooth muscle cells instead, which are partially involved in the formation of new capillaries in cardiac repair. To test this hypothesis, we used human BM-CD133 + cells that we had previously characterized and arterioles.…”

Section: Discussionmentioning

confidence: 99%

Human Bone Marrow-Derived CD133⁺Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis

et al. 2010

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Transplanting hematopoietic and peripheral blood-derived stem/progenitor cells can have beneficial effects in slowing the effects of heart failure. We investigated whether human bone marrow CD133+ -derived cells (BM-CD133 + cells) might be used for cell therapy of heart injury in combination with tissue engineering. We examined these cells for: 1) their in vitro capacity to be converted into cardiomyocytes (CMs), and 2) their potential for in vivo differentiation when delivered to a tissue-engineered type I collagen patch placed on injured hearts (group II). To ensure a microvascular network ready for use by the transplanted cells, cardiac injury and patching were scheduled 2 weeks before cell injection. The cardiovascular potential of the BM-CD133 + cells was compared with that of a direct injection (group I) of the same cells in heart tissue damaged according to the same schedule as for group II. While a small fraction (2 ± 0.5%) of BM-CD133 + cells cocultured with rat CMs switched in vitro to a CM-like cell phenotype, in vivo-and in both groups of nude rats transplanted with BM-CD133 + -there was no evidence of any CM differentiation (as detected by cardiac troponin I expression), but there were signs instead of new capillaries and small arterioles. While capillaries prevailed over arterioles in group II, the opposite occurred in group I. The transplanted cells further contributed to the formation of new microvessels induced by the patch (group II) but the number of vessels did not appear superior to the one developed after directly injecting the BM-CD133 + cells into the injured heart. Although chimeric human-rat microvessels were consistently found in the hearts of both groups I and II, they represented a minority (1.5-2.3%) compared with those of rat origin. Smooth muscle myosin isoform expression suggested that the arterioles achieved complete differentiation irrespective of the presence or absence of the collagen patch. These findings suggest that: 1) BM-CD133 + cells display a limited propensity for in vitro conversion to CMs; 2) the preliminarily vascularized bioscaffold did not confer a selective homing and differentiation advantage for the phenotypic conversion of BM-CD133 + cells into CMs; and 3) combined patching and cell transplantation is suitable for angiogenesis and arteriogenesis, but it does not produce better results, in terms of endothelial and smooth muscle cell differentiation, than the "traditional" method of cell injection into the myocardium.

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Section: Discussionmentioning

confidence: 99%

Human Bone Marrow-Derived CD133⁺Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis

et al. 2010

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“…Concerning myocardium, muscle and nonmuscle cell population from multipotent progenitors located in the first and second heart fields contribute to cardiogenesis [9,10], tangling the identification of the resident progenitor cells actually involved in myocardial homeostasis throughout lifetime [11]. Cells expressing c-kit/CD117 have been acknowledged as the ''true cardiomyocyte progenitors'' [12]; however, Sca-1 has been recently shown to be a marker identifying a murine [13] and a human [14] heart cell population with cardiomyogenic potential, since both could generate new cardiomyocytes in vitro and in vivo on specific treatments [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

et al. 2011

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Human heart harbors a population of resident progenitor cells that can be isolated by stem cell antigen-1 antibody and expanded in culture. These cells can differentiate into cardiomyocytes in vitro and contribute to cardiac regeneration in vivo. However, when directly injected as single cell suspension, less than 1%-5% survive and differentiate. Among the major causes of this failure are the distressing protocols used to culture in vitro and implant progenitor cells into damaged hearts. Human cardiac progenitors obtained from the auricles of patients were cultured as scaffoldless engineered tissues fabricated using temperature-responsive surfaces. In the engineered tissue, progenitor cells established proper three-dimensional intercellular relationships and were embedded in self-produced extracellular matrix preserving their phenotype and multipotency in the absence of significant apoptosis. After engineered tissues were leant on visceral pericardium, a number of cells migrated into the murine myocardium and in the vascular walls, where they integrated in the respective textures. The study demonstrates the suitability of such an approach to deliver stem cells to the myocardium. Interestingly, the successful delivery of cells in murine healthy hearts suggests that myocardium displays a continued cell cupidity that is strictly regulated by the limited release of progenitor cells by the adopted source. When an unregulated cell source is added to the system, cells are delivered to the myocardium. The exploitation of this novel concept may pave the way to the setup of new protocols in cardiac cell therapy.

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“…The heart is composed of multiple cell types, including cardio myocytes (CMs), endothelial cells (ECs), and smooth muscle cells (SMCs;Martin Puig et al, 2008). The mammalian heart is divided in four chambers: two atria and two ventricles, which are con nected to the pulmonary and the general circulation by four vessels (Olson, 2006).…”

Section: Introductionmentioning

confidence: 99%

Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation

Bondue¹,

Tännler²,

Chiapparo³

et al. 2011

J Exp Med

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Lives of a Heart Cell: Tracing the Origins of Cardiac Progenitors

Cited by 182 publications

References 97 publications

Human Bone Marrow-Derived CD133⁺Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis

Human Bone Marrow-Derived CD133⁺Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation

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Lives of a Heart Cell: Tracing the Origins of Cardiac Progenitors

Cited by 182 publications

References 97 publications

Human Bone Marrow-Derived CD133+Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis

Human Bone Marrow-Derived CD133+Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation

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Human Bone Marrow-Derived CD133⁺Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis

Human Bone Marrow-Derived CD133⁺Cells Delivered to a Collagen Patch on Cryoinjured Rat Heart Promote Angiogenesis and Arteriogenesis