Liver transcriptome analysis reveals biological pathways and transcription factors in response to high ammonia exposure

Li, Daojie; Chen, Shuangzhao; Li, Chun; Wei, Baoxing; Li, Xiaoping

doi:10.1080/08958378.2022.2083275

Cited by 2 publications

(1 citation statement)

References 62 publications

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“…Meanwhile, the expression of gck , which is the rate-limiting enzyme in glycolysis from glucose to pyruvate, and the expression of idh , which is involved in the TCA cycle, were significantly decreased after ammonia exposure. This is also in accordance with a previous study in pigs in which glycolysis-related genes were also down-regulated during ammonia exposure [ 38 ] and in brain cells whose TCA cycle reactions were inhibited during ammonia exposure [ 39 ]. Moreover, the pentose phosphate pathway was also inhibited with the significantly down-regulated expression of g6pd after ammonia exposure.…”

Section: Discussionsupporting

confidence: 93%

Liver Injury and Metabolic Dysregulation in Largemouth Bass (Micropterus salmoides) after Ammonia Exposure

Zou

Wang

et al. 2023

Metabolites

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Elevated environmental ammonia leads to respiratory disorders and metabolic dysfunction in most fish species, and the majority of research has concentrated on fish behavior and gill function. Prior studies have rarely shown the molecular mechanism of the largemouth bass hepatic response to ammonia loading. In this experiment, 120 largemouth bass were exposed to total ammonia nitrogen of 0 mg/L or 13 mg/L for 3 and 7 days, respectively. Histological study indicated that ammonia exposure severely damaged fish liver structure, accompanied by increased serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activity. RT-qPCR results showed that ammonia exposure down-regulated the expression of genes involved in glycogen metabolism, tricarboxylic acid cycle, lipid metabolism, and urea cycle pathways, whereas it up-regulated the expression of genes involved in gluconeogenesis and glutamine synthesis pathways. Thus, ammonia was mainly converted to glutamine in the largemouth bass liver during ammonia stress, which was rarely further used for urea synthesis. Additionally, transcriptome results showed that ammonia exposure also led to the up-regulation of the oxidative phosphorylation pathway and down-regulation of the mitogen-activated protein kinase signaling pathway in the liver of largemouth bass. It is possible that the energy supply of oxidative phosphorylation in the largemouth bass liver was increased during ammonia exposure, which was mediated by the MAPK signaling pathway.

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Section: Discussionsupporting

confidence: 93%