Liver transcriptome analysis and cortisol immune-response modulation in lipopolysaccharide-stimulated in channel catfish (Ictalurus punctatus)

Jiang, Hucheng; Wang, Minghua; Fu, Ling; Zhong, Liqiang; Liu, Guoxing; Chen, Xiaohui; Bian, Wenji

doi:10.1016/j.fsi.2020.03.024

Cited by 17 publications

(15 citation statements)

References 61 publications

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“…qRT-PCR was used to confirm the expression levels of DEGs identified by RNA-seq analysis. Primers used are listed in Table 1 (43)(44)(45)(46)(47)(48). In channel catfish, EF-1 a was used as an endogenous control whereas gyrB was used for Y. ruckeri.…”

Section: Quantitative Real-time Reverse-transcription Pcr (Qrt-pcr)mentioning

confidence: 99%

Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions

et al. 2021

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Host-pathogen intectarions are complex, involving large dynamic changes in gene expression through the process of infection. These interactions are essential for understanding anti-infective immunity as well as pathogenesis. In this study, the host-pathogen interaction was analyzed using a model of acute infection where channel catfish were infected with Yersinia ruckeri. The infected fish showed signs of body surface hyperemia as well as hyperemia and swelling in the trunk kidney. Double RNA sequencing was performed on trunk kidneys extracted from infected channel catfish and transcriptome data was compared with data from uninfected trunk kidneys. Results revealed that the host-pathogen interaction was dynamically regulated and that the host-pathogen transcriptome fluctuated during infection. More specifically, these data revealed that the expression levels of immune genes involved in Cytokine-cytokine receptor interactions, the NF-kappa B signaling pathway, the JAK-STAT signaling pathway, Toll-like receptor signaling and other immune-related pathways were significantly upregulated. Y. ruckeri mainly promote pathogenesis through the flagellum gene fliC in channel catfish. The weighted gene co-expression network analysis (WGCNA) R package was used to reveal that the infection of catfish is closely related to metabolic pathways. This study contributes to the understanding of the host-pathogen interaction between channel catfish and Y. ruckeri, more specifically how catfish respond to infection through a transcriptional perspective and how this infection leads to enteric red mouth disease (ERM) in these fish.

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Section: Quantitative Real-time Reverse-transcription Pcr (Qrt-pcr)mentioning

confidence: 99%

Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions

et al. 2021

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“…Liver was collected from all individuals and stored in RNAlater R . This organ was chosen because it plays a critical role in the coordination of innate and adaptive immune responses (Kumar et al, 2017;Rebl and Goldammer, 2018), and it has been used in several studies involving transcriptome analysis in fish for a better understanding of the behavior of the immune system in face of bacterial infections (Tran et al, 2015;Maekawa et al, 2019;Jiang et al, 2020).…”

Section: Tissue Collection Rna Isolation and Sequencingmentioning

confidence: 99%

“…To control false discovery rates of multiple-hypothesis testing, the p-values calculated by DESeq2 were processes to q-values. Then, the list of differentially expressed transcripts were generated with cutoff q-values less than 0.05 and log 2 FC (fold change (condition/control) for a gene) > 1 or log 2 FC < −1) as described in similar transcriptome studies (Marancik et al, 2015;Polinski et al, 2016;Jiang et al, 2020).…”

Section: Data Processing and Analysismentioning

confidence: 99%

Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response

et al. 2020

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Pacu (Piaractus mesopotamicus) is a Neotropical fish of major importance for South American aquaculture. Septicemia caused by Aeromonas hydrophila bacteria is currently considered a substantial threat for pacu aquaculture that have provoked infectious disease outbreaks with high economic losses. The understanding of molecular aspects on progress of A. hydrophila infection and pacu immune response is scarce, which have limited the development of genomic selection for resistance to this infection. The present study aimed to generate information on transcriptome of pacu in face of A. hydrophila infection, and compare the transcriptomic responses between two groups of time-series belonging to a disease resistance challenge, peak mortality (HM) and mortality plateau (PM) groups of individuals. Nine RNA sequencing (RNA-Seq) libraries were prepared from liver tissue of challenged individuals, generating ∼160 million 150 bp pair-end reads. After quality trimming/cleanup, these reads were assembled de novo generating 211,259 contigs. When the expression of genes from individuals of HM group were compared to individuals from control group, a total of 4,413 differentially expressed transcripts were found (2,000 upregulated and 2,413 downregulated candidate genes). Additionally, 433 transcripts were differentially expressed when individuals from MP group were compared with those in the control group (155 upregulated and 278 downregulated candidate genes). The resulting differentially expressed transcripts were clustered into the following functional categories: cytokines and signaling, epithelial protection, antigen processing and presentation, apoptosis, phagocytosis, complement system cascades and pattern recognition receptors. The proposed results revealing relevant differential gene expression on HM and PM groups which will contribute to a better understanding of the molecular defense mechanisms during A. hydrophila infection.

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“…The physical stresses such as uncomfortable water environment, feeding regimen and handling crowding have been reported to greatly affect the whole‐body cortisol level in fish (Ramsay et al, 2006; Williams et al, 2017; Zhang et al, 2020). In addition, as reported in rainbow trout and Atlantic salmon, cortisol changes are closely related to pathogen infections and can lead to not only the cortisol elevation in fish body but also the cortisol release into the water outside, which provides a convenient pathway for early warning of acute disease outbreaks in fish farming (Ellis et al, 2007; Jiang et al, 2020).…”

Section: Introductionmentioning

confidence: 97%

Developmental changes of the digestive capacity and hormones levels in developing embryos and yolk‐sac larvae of tongue sole, Cynoglossus semilaevis

Jia

et al. 2021

Aquac Res

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Developmental changes in enzymatic activities of alkaline phosphatase (AKP), acid phosphatase (ACP), amylase (AMY), pyruvate kinase (PK), Leucine aminopeptidase N (LAP), Lipase (LIP), Glutamic oxalo acetic transaminase (GOT), Lactate dehydrogenase (LDH), Chymotrypsin (CHY) and Trypsin (TRY) and levels of thyroid hormones and cortisol were measured in developing embryos and yolk‐sac larvae of tongue sole, Cynoglossus semilaevis. During embryonic developmental periods, the fastest growth occurred in blastula stage and hatching appeared at 38.8 hours after fertilization (HAF). Besides PK which appeared in cleavage stage onwards, the other enzymes were observed in fertilized egg. Changeable patterns of enzymatic activities presented five types including upward trend for AMY and CHY, M‐shaped trend for LIP and PK, V‐shaped trend for LAP and GOT, W‐shaped trend for LDH and TRY, and an oscillation trend for ACP and AKP. As for hormones, patterns were M‐shaped trend for T3, oscillation trend for T4 and downward trend for cortisol. Furthermore, LDH, LIP, PK, TRY, ACP, GOT and T4 showed relatively high levels in blastula stage, which may be an important factor leading to the most rapid growth of embryo. Regression analysis revealed that AMY, GOT and ACP were significantly correlated with embryonic mass, which may provide an effective way to evaluate development of embryos. During yolk‐sac larval periods, mouth‐opening day appeared at 3 days after hatching (DAH). Besides LAP, GOT, T4 and cortisol which showed downward tendency, the other enzymes and T3 displayed upward tendency and reached the peak at 3 DAH, preparing well for upcoming exogenous digestion.

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Liver transcriptome analysis and cortisol immune-response modulation in lipopolysaccharide-stimulated in channel catfish (Ictalurus punctatus)

Cited by 17 publications

References 61 publications

Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions

Dual RNA-Seq of Trunk Kidneys Extracted From Channel Catfish Infected With Yersinia ruckeri Reveals Novel Insights Into Host-Pathogen Interactions

Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With Pathogenic Aeromonas hydrophila: Inference on Immune Gene Response

Developmental changes of the digestive capacity and hormones levels in developing embryos and yolk‐sac larvae of tongue sole, Cynoglossus semilaevis

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