Liver repopulation with hepatocyte transplantation: new avenues for gene and cell therapy

Gupta, Sanjeev; Malhi, Harmeet; Singh, Gaurav; Novikoff, Phyllis M.

doi:10.1002/(sici)1521-2254(199911/12)1:6<386::aid-jgm73>3.0.co;2-z

Cited by 19 publications

(11 citation statements)

References 41 publications

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“…In principle, a single donor human liver should generate sufficient numbers of LSECs to treat multiple patients if only 10% of the LSECs were needed for transplantation. Reisolation of up to 0.5% transplanted LSECs in NPCs from mice without MCT preconditioning suggested that only 10% of the transplanted LSECs engrafted, which was generally similar to the engraftment efficiency of primary hepatocytes in the liver (37). On the other hand, pretreatment of mice with FLP and MCT, especially MCT, significantly enhanced LSEC engraftment, also resembling the effect of MCT on the engraftment of transplanted hepatocytes, which too is facilitated by the disruption of the sinusoidal endothelial barrier (17)(18)(19).…”

Section: Discussionmentioning

confidence: 64%

Transplanted endothelial cells repopulate the liver endothelium and correct the phenotype of hemophilia A mice

et al. 2008

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Section: Discussionmentioning

confidence: 64%

Transplanted endothelial cells repopulate the liver endothelium and correct the phenotype of hemophilia A mice

et al. 2008

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“…27,28 Furthermore, the potential ability to replace the liver with genetically modified disease-resistant hepatocytes may offer new insights into correcting genetic disorders and treating patients with chronic liver diseases. 29,30 Although the use of human ES cells for therapeutic purposes must 31,32 to date, convincing results have remained elusive. According to a report by Sell, 33 3 types of liver progenitor cells were identified in human atypical ductular reactions: type I, primitive; type II, bile ductlike; type III, hepatocyte-like.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of embryonic stem cells into hepatocytes: Biological functions and therapeutic application

Yamamoto¹,

et al. 2003

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Embryonic stem (ES) cells provide a unique source for tissue regeneration. We examined whether mouse ES cells can efficiently differentiate into transplantable hepatocytes. ES cells were implanted into mouse livers 24 hours after carbon tetrachloride intoxication; ES-derived cells with several hepatocyte-cell-markers were generated. They were able to grow in vitro and showed morphology consistent with typical mature hepatocytes and expressed hepatocyte-specific genes. After transplantation into the carbon tetrachloride-injured mouse liver, ES-derived green fluorescent protein-positive cells were incorporated into liver tissue and rescued mice from hepatic injury. No teratoma formation was observed in the transplant recipients. In conclusion, ES cells can provide a valuable tool for studying the molecular basis for differentiation of hepatocytes and form the basis for cell therapies. (HEPATOLOGY 2003;37:983-993.)

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“…[16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] Promising new vector systems have emerged, among which HSV based vectors seem to display all the characteristics to transfer genes efficiently to hepatocytes. 20,22,26,29 The results presented here (a) add new evidences to the hypothesis that HSV-1 binding to the cells can be re-directed; and (b) support the assumption that preS1ap is a molecule that HBV uses to bind to the hepatocytes.…”

Section: Discussionmentioning

confidence: 99%

“…By far, the most successful have been those using the retroviral vectors. 16,[18][19][20][21][22][23][24][25][26][27][28][29][30] However, retroviral-mediated gene transfer is relatively inefficient. The inefficiency of retroviral vectors as vehicles for hepatocyte gene therapy results from the fact that these vectors require a replicating cell for stable expression.…”

Section: Introductionmentioning

confidence: 99%

Specific targeted binding of herpes simplex virus type 1 to hepatocytes via the human hepatitis B virus preS1 peptide

et al. 2004

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To improve the utility of herpes simplex virus type 1 (HSV-1) vectors for gene therapy, the viral envelope needs to be manipulated to achieve cell-specific gene delivery. In this report, we have engineered an HSV-1 mutant virus, KgBpK À gC À , deleted for the glycoprotein C (gC) and the heparan sulfate-binding domain (pK) of gB, in order to express gC:preS1 and gC:preS1 active peptide (preS1ap) fusion molecules. PreS1, and a 27 amino acid active peptide inside preS1 (preS1ap), are supposed to be the molecules that the human hepatitis B virus (HBV) needs to bind specifically to hepatocytes. Biochemical analysis demonstrated that the gC:preS1ap fusion molecule was expressed and incorporated into the envelope of the recombinant HSV-1 virus KgBpK À gC:preS1ap. Moreover, KgBpK À gC:preS1ap recombinant virus gained a specific binding activity to an hepatoblastoma cell line (HepG2) with a consequent productive infection. In addition, anti-preS1-specific antibodies were shown to neutralize recombinant virus infectivity, and a synthetic preS1ap peptide was able to elute KgBpK À gC:pregC:preS1ap virus bound on HpeG2 cells. These data provide further evidence that HSV-1 can productively infect cells through a specific binding to a non-HSV-1 receptor. Furthermore, these data strongly support the hypothesis that the HBV preS1ap molecule is an HBV ligand to hepatocytes.

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Liver repopulation with hepatocyte transplantation: new avenues for gene and cell therapy

Cited by 19 publications

References 41 publications

Transplanted endothelial cells repopulate the liver endothelium and correct the phenotype of hemophilia A mice

Transplanted endothelial cells repopulate the liver endothelium and correct the phenotype of hemophilia A mice

Differentiation of embryonic stem cells into hepatocytes: Biological functions and therapeutic application

Specific targeted binding of herpes simplex virus type 1 to hepatocytes via the human hepatitis B virus preS1 peptide

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