Liver regeneration

Fausto, Nelson; Campbell, Jean S.; Riehle, Kimberly J.

doi:10.1016/j.jhep.2012.04.016

Cited by 167 publications

(166 citation statements)

References 12 publications

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“…In a normal physiological state, approximately 0.0012-0.01% of rat liver cells are undergoing mitosis (Fausto et al, 2012;Michalopoulos, 2014). After injury or surgical removal of the cause of injury, liver cells quickly begin to proliferate and recover the lost organ weight (Cienfuegos et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate

2016

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ABSTRACT. This study investigated the efficacy and feasibility of inducing the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro using Sprague Dawley rats, as a model of hepatocyte generation for cell transplantation. BMSCs were isolated and grown using the adherent method and exposed to 5 or 10% liver tissue homogenate, before being collected for analysis after 0, 7, 14, and 21 days. Immunofluorescence and western blotting were employed to detect the liver-specific markers a-fetoprotein (AFP) and albumin (ALB). Supernatant urea content was also measured to verify that differentiation had been induced. After 7 days in the presence of 10% liver tissue homogenate, BMSCs demonstrated hepatocyte-like morphological characteristics, and with prolonged culture time, liverspecific markers were gradually produced at levels indicating cell maturation. AFP expression peaked at 14 days then began to decrease, while both urea and ALB levels increased with induction time. Overall, 2 X.K. Xing et al. Genetics and Molecular Research 15 (3): gmr.15038550 marker expression in the 5% homogenate group was less than or equal to the 10% group at each time point. Thus, in a rat model, liver tissue homogenate obtained from partial hepatectomy can induce the differentiation of BMSCs into hepatocyte-like cells. This method is simple, feasible, and has remarkable real-world application potential.

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Section: Discussionmentioning

confidence: 99%

Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate

2016

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“…Un nombre abondant de publications montrent que la voie de l'EGFR (epidermal growth factor receptor) intervient dans la régénération du foie [1][2][3][4]. Cependant, les mécanismes l'impliquant dans la carcinogenèse restent largement méconnus.…”

Section: Quel Rôle Pour L'egfr Dans La Régénération Et La Carcinogenèunclassified

“…La problématique soulevée dans l'article de Nature publié par les chercheurs de l'institut Curie (Paris) [4] est la suivante : tester si la pression mécanique de croissance tumorale peut engendrer des phénomènes de tumorigenèse dans les tissus sains (non tumoraux) avoisinant la tumeur, au sein d'un organisme in vivo. Un groupe de chercheurs a mis au point une approche tout à fait novatrice afin d'étudier les conséquences, sur les tissus avoisinants, de la pression mécanique générée au cours de la croissance tumorale [4]. Les auteurs ont analysé les voies de transduction activées dans les cellules subissant cette force, et se sont intéressés plus particulièrement à la voie de la -caténine.…”

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EGFR, régénération et carcinogenèse hépatiques : quelle responsabilité ?

2016

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> Le carcinome hépatocellulaire est le septième cancer le plus répandu dans le monde. Plusieurs facteurs environnementaux, comme la consommation d'alcool ou les infections par les virus des hépatites B ou C, favorisent son apparition, ce qui en fait une priorité de santé publique. En effet, une régénération prolongée des hépatocytes, souvent en relation avec une hépatopathie chronique, engendre des lésions génétiques et épigénétiques des cellules et un déséquilibre de la balance apoptose -prolifération des hépatocytes qui facilitent l'émergence d'une tumeur. Quel rôle pour l'EGFR dans la régénération et la carcinogenèse hépatiques ?Un nombre abondant de publications montrent que la voie de l'EGFR (epidermal growth factor receptor) intervient dans la régénération du foie [1][2][3][4]. Cependant, les mécanismes l'impliquant dans la carcinogenèse restent largement méconnus.

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“…Liver cells are quiescent under normal conditions; however, they enter the cell cycle when damaged and proliferate until the original liver volume is restored (1). A number of conditions may alter liver mass, including surgical resection, chemicals and pathogens (2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

“…In cellular proliferation, cMyc and cyclin D1 genes or proteins are downregulated, while the expression of p15, p21 and Smad2 genes is upregulated following activation of the TGF-β/Smad pathway. In non-Smad pathways, the TGF-β receptor activates Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model SEUNG-HO HEO 1,2 , EUI-SUK JEONG 1,3 , KYOUNG-SUN LEE 1,4 , JIN-HEE SEO 1,5 , WOON-KYU LEE 6 and YANG-KYU CHOI other proteins, including RAS, phosphoinositide 3-kinase and FAS (12,(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model

et al. 2017

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Abstract. The liver has marked regenerative capabilities, and numerous signaling pathways are involved in liver regeneration. The transforming growth factor-β (TGF-β)/Smad pathway, which is also involved in liver regeneration, regulates numerous biological processes. Krüppel-like factor 10 (KLF10) has been reported to activate the TGF-β/Smad signaling pathway; however, the exact functions of KLF10 under various pathophysiological conditions remain unclear. In the present study, the role of KLF10 in liver regeneration following partial hepatectomy (PH) was investigated using KLF10-knockout (KO) mice. KLF10-KO mice exhibited lower liver/body weight ratios and 5-bromo-2-deoxy-uridine labeling indices compared with wild-type (WT) mice, and significant differences (P= 0.028) were obtained at 72 h after PH. To understand the causes of the gross and histopathological findings, the expression levels of the components of the TGF-β/Smad pathway were examined using reverse transcription-quantitative polymerase chain reaction and western blot analysis. The mRNA and protein levels of Smad3, p15, TGF-β1 and TGF-β receptor 1 were significantly increased, while those of cMyc and cyclin D1 (proliferation-associated genes) were significantly lower in the liver tissues of the KLF10-KO mice compared with those of the WT mice at 72 h post-PH. These results indicated that KLF10-KO may exhibit antiproliferative effects on liver regeneration following PH, through strengthening the TGF-β/Smad signaling pathway in a delayed manner.

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Liver regeneration

Cited by 167 publications

References 12 publications

Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate

Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate

EGFR, régénération et carcinogenèse hépatiques : quelle responsabilité ?

Knockout of krüppel-like factor 10 suppresses hepatic cell proliferation in a partially hepatectomized mouse model

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