Liver of the brown trout,Salmo trutta (Teleostei, Salmonidae): A stereological study at light and electron microscopic levels

The hepatocytes of the brown trout (Salmo trutta f. fario): a stereological study of their number and size during the breeding cycle

“…Anyway, our data support that a numerical decline of hepatocytes in females from vitellogenesis to post-spawning occurred. Moreover, it agrees with our previous findings that females at endogenous vitellogenesis (in April) had a larger relative volume of macrophages (Rocha et al 1997) and a higher total volume of dense bodies (Rocha et al 2001). At both occasions, we conjectured that it could be related with liver remodeling after breeding, in agreement with the hypothesised for rainbow trout (Hampton et al 1989).…”

“…This is true for most situations (Weibel 1979;Hanstede and Gerrits 1983;Gerrits et al 1991), and applies to our material (Rocha et al 1997. The V V (parenchyma, liver), as well as two additional volume density estimations (see below), were obtained by an unbiased stereological technique based on point counting (e.g., Weibel 1979):…”

“…Despite great basic differences as to how hepatocytes are three-dimensionally arranged (Hinton and Couch 1998;Hardman et al 2007), from fishes to mammals they are the main structural component of the liver parenchyma. In the relatively few species studied, hepatocytes make up around 80% of the liver volume in both fishes and mammals (e.g., Weibel et al 1969;Rocha et al 1997).…”

“…The use of stereology in hepatology dates from long ago, namely in mammalian liver studies (e.g., Loud 1968; Weibel et al 1969). True stereological studies in fish livers are much more recent (e.g., Hinton et al 1984;Hampton et al 1989), but are also being increasingly used in experiments (e.g., Moutou et al 1997;Schramm et al 1998;Figueiredo-Fernandes et al 2006) and in other conditions (e.g., Rocha et al 1995Rocha et al , 1997Rocha et al , 2001.…”

Zebrafish liver (ZFL) cells are able to mount an anti-viral response after stimulation with Poly (I:C)

“…The presence of resident phagocytic immune cells, equivalent to the mammalian Kupffer cells, has been demonstrated only in some teleost species, and their function is still not known (Mulero et al, 2008;ller et al, 2014). Besides, in some of the teleost species that do contain resident immune cells, their presence has been proved to be markedly lower than in mammalian livers (Rocha et al, 1997;ller et al, 2014). In zebrafish, Kupffer cells have not yet been identified (Chu and Sadler, 2009).…”