Liver of juvenile atlantic salmon, Salmo salar L.: A light, transmission, and scanning electron microscopic study, with special reference to the sinusoid

Abstract

The sinusoids of Atlantic salmon liver are lined by a fenestrated endothelium, with PSC located in the space of Disse, with macrophages and IHC as inhabitants of the interhepatocytic space. IHC show ultrastructural similarities to mammalian pit cells and teleostean large granular lymphocytes, as well as to piscine monocytes. PSC might be storage cells for vitamin A in Atlantic salmon as shown by autofluorescence in these cells, while immunohistochemical studies indicate that desmin does not seem to be an adequate immunohistochemical marker for PSC in the juvenile Atlantic salmon. Methodologically, fixation for electron microscopy was performed by a new and convenient perfusion method: arterial retrograde whole body perfusion. Liver specimens intended for scanning electron microscopy were fractured at room temperature after prolonged osmium postfixation, leaving hepatocytes intact and producing images well suited to document the three-dimensional structure of cells and tissue.

Comparative histological study of hepatic architecture in the three orders amphibian livers

“…This classification is based on the investigation of Elias and Bengelsdorf in several vertebrate animals [2]. Previous studies described that some fish had a similar structure to normal humans, while others were modified in a more primitive form [3,21]. Our study of 46 species showed that the primitive form was a combination of several-cell-thick plate and two-cell-thick plate types in the genus Hynobius.…”

Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions

“…However, details of these structures were not given and not further discussed. In the literature, terms such as slender filopodia [34, 35], or slender microvilli [36, 37] have been used. All filopodia and microvilli are morphologically slender and these descriptive terms in general have not been used to refer to microextensions having a particular dimension.…”

What happens to the DNA vaccine in fish? A review of current knowledge

“…Fish macrophages are also located in several other tissues such as spleen, thymus, intestine and mesentery [233]. However, fish seem to lack the resident sinusoidal macrophages in their livers [234]. The macrophages of fish are not as well studied as their mammalian counterpart, although they seem to carry out much of the same functions [235] (see above), e.g.…”