Liver glycogen metabolism during short‐term insulin‐induced hypoglycemia in fed rats

Obici, Simoni; Lopes-Bertolini, Gisele; Curi, Rui; Bazotte, Roberto Barbosa

doi:10.1002/cbf.1501

Cited by 9 publications

(4 citation statements)

References 19 publications

(27 reference statements)

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“…5,6 Thus, by using this experimental model we demonstrated that during short-term IIH the ability to produce acetoacetate and ß-hydroxybutyrate from octanoate in livers of non-diabetic rats was maintained. 6 On the other hand, studies showing the effect of IIH on the liver capacity in producing ketone bodies in diabetic rats submitted to hypoglycemia are lacking.…”

Section: Introductionmentioning

confidence: 79%

Ketogenesis evaluation in perfused liver of diabetic rats submitted to short‐term insulin‐induced hypoglycemia

Barrena

Gazola

Furlan

et al. 2009

Cell Biochemistry & Function

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Ketogenesis, inferred by the production of acetoacetate plus ss-hydroxybutyrate, in isolated perfused livers from 24-h fasted diabetic rats submitted to short-term insulin-induced hypoglycemia (IIH) was investigated. For this purpose, alloxan-diabetic rats that received intraperitoneal regular insulin (IIH group) or saline (COG group) injection were compared. An additional group of diabetic rats which received oral glucose (gavage) (100 mg kg(-1)) 15 min after insulin administration (IIH + glucose group) was included. The studies were performed 30 min after insulin (1.0 U kg(-1)) or saline injection. The ketogenesis before octanoate infusion was diminished (p < 0.05) in livers from rats which received insulin (COG vs. IIH group) or insulin plus glucose (COG vs. IIH + glucose group). However, the liver ketogenic capacity during the infusion of octanoate (0.3 mM) was maintained (COG vs. IIH group and COG vs. IIH + glucose group). In addition, the blood concentration of ketone bodies was not influenced by the administration of insulin or insulin plus glucose. Taken together, the results showed that inspite the fact that insulin and glucose inhibits ketogenesis, livers from diabetic rats submitted to short-term IIH which received insulin or insulin plus glucose showed maintained capacity to produce acetoacetate and ss-hydroxybutyrate from octanoate.

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Section: Introductionmentioning

confidence: 79%

Ketogenesis evaluation in perfused liver of diabetic rats submitted to short‐term insulin‐induced hypoglycemia

Barrena

Gazola

Furlan

et al. 2009

Cell Biochemistry & Function

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“…The in situ liver perfusion technique was done as previously described, and the glucose production from l ‐alanine (5 mM), l ‐glutamine (5 mM), l ‐lactate (2 mM) and glycerol (2 mM) in livers from the COG and the HFD groups were compared.…”

Section: Methodsmentioning

confidence: 99%

“…The in situ liver perfusion technique was done as previously described, 18,19 and the glucose production from L-alanine (5 mM), L-glutamine (5 mM), L-lactate (2 mM) and glycerol (2 mM) in livers from the COG and the HFD groups were compared. Figure 1 shows demonstrative experiments in which after a pre-infusion period (10 min) the gluconeogenic substrate was infused during 60 min, followed by a post-infusion period (10 min without the gluconeogenic substrate) to allow the return of basal glucose production values of the pre-infusion period.…”

Section: Liver Perfusion Experimentsmentioning

confidence: 99%

Time sequence of the intensification of the liver glucose production induced by high‐fat diet in mice

Obici

Tavoni

Barrena

et al. 2012

Cell Biochemistry & Function

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It is well established that the development of insulin resistance shows a temporal sequence in different organs and tissues. Moreover, considering that the main aspect of insulin resistance in liver is a process of glucose overproduction from gluconeogenesis, we investigated if this metabolic change also shows temporal sequence. For this purpose, a well-established experimental model of insulin resistance induced by high-fat diet (HFD) was used. The mice received HFD (HFD group) or standard diet (COG group) for 1, 7, 14 or 56 days. The HFD group showed increased (P < 0.05 versus COG) epididymal, retroperitoneal and inguinal fat weight from days 1 to 56. In agreement with these results, the HFD group also showed higher body weight (P < 0.05 versus COG) from days 7 to 56. Moreover, the changes induced by HFD on liver gluconeogenesis were progressive because the increment (P < 0.05 versus COG) in glucose production from l-lactate, glycerol, l-alanine and l-glutamine occurred 7, 14, 56 and 56 days after the introduction of the HFD schedule, respectively. Furthermore, glycaemia and cholesterolemia increased (P < 0.05 versus COG) 14 days after starting the HFD schedule. Taken together, the results suggest that the intensification of liver gluconeogenesis induced by an HFD is not a synchronous 'all-or-nothing process' but is specific for each gluconeogenic substrate and is integrated in a temporal manner with the progressive augmentation of fasting glycaemia.

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“…Livers of rats fasted for 15 h were perfused in situ as previously described (Obici et al 2008). After a 10 min pre-perfusion period, the gluconeogenic substrate L-glutamine, under saturating concentration (5 mM), was dissolved into the perfusion fluid and infused during 50 min.…”

Section: Liver Perfusionmentioning

confidence: 99%

Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study

Siqueira

Batistela

Pereira

et al. 2016

Pharmaceutical Biology

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Context Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/ kg ClEtOH (DT500). After 21 d of treatment, hepatic glucose and urea production were investigated through in situ perfused liver with L-glutamine. Changes in the phosphoenolpyruvate carboxykinase (PEPCK) levels and in the activation of adenosine monophosphate-activated protein kinase (AMPK) and insulin-signalling intermediates were also investigated. Results Similar to DMet, DT500 rats showed a reduction in the rates of hepatic production of glucose (46%) and urea (22%) in comparison with DC. This reduction was accompanied by a reduction in the PEPCK levels in liver of DT500 (28%) and DMet (43%) when compared with DC. AMPK phosphorylation levels were higher in the liver of DT500 (17%) and DMet (16%) rats. The basal AKT phosphorylation levels were increased in liver of DT500 rats, without differences in the insulin-stimulated AKT phosphorylation and in the insulin receptor levels between DC and DT500 rats. Discussion and conclusion The antidiabetic activity of ClEtOH can be attributed, at least in part, to inhibition of hepatic gluconeogenesis, probably due to the activation of both AMPK and AKT effectors and reduction in the PEPCK levels.ARTICLE HISTORY

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Liver glycogen metabolism during short‐term insulin‐induced hypoglycemia in fed rats

Cited by 9 publications

References 19 publications

Ketogenesis evaluation in perfused liver of diabetic rats submitted to short‐term insulin‐induced hypoglycemia

Ketogenesis evaluation in perfused liver of diabetic rats submitted to short‐term insulin‐induced hypoglycemia

Time sequence of the intensification of the liver glucose production induced by high‐fat diet in mice

Combretum lanceolatum flowers ethanol extract inhibits hepatic gluconeogenesis: an in vivo mechanism study

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