Liver Function Status in Male Wister Rats Treated with Ethanol Extract of the Leaves of Nauclea latifolia Owing to Aluminum Chloride-Induced Oxidative Stress

Abstract

Aim: The goal of this study is to determine the protective effects of N. latifolia ethanol leaf extracts against toxicity caused by aluminum chloride (AlCl 3 ) in male rats. Materials and methods:Twenty male albino rats were randomly divided into four groups of five animals and studied over a 7-day period. The first group served as the control and received only normal feed and water, Group 2 received AlCl 3 (100mg/kg bw) daily. Group 3 received 100mg/kg bw ethanol extract of N. latifolia an hour after administration of 100mg/kg AlCl 3 . Group 4 was treated with only ethanol extract of N. latifolia (100mg/kg bw).Results: Thiobarbituric acid reactive substances (TBARS), bilirubin, alanine aminotransferase, aspartate aminotransferase and full blood count were significantly (p < 0.05) changed in rats treated with AlCl 3 (100mg/kg bw). The results obtained indicate that the extracts were beneficial in ameliorating damages caused by AlCl 3 in male rats. Conclusion:This study clearly showed the protective effects of N. latifolia extracts on liver mal-function by aluminum chloride induced in male rats. The obtained results indicated that the N. latifolia at 100mg/kg bw would be a good natural source for protection against mal-functioning of the liver in male rats. Copyright: ©2016 Yakubu ethanol (500ml) in the ratio (1:5 w/v) with intermittent shaking for exactly 48hrs. The extract was filtered out first using a clean white sieving mesh and Whatman No. 1 filter paper. The filtrates were concentrated using a thermostat water cabinet at 40°C for 7 days. The concentrated extracts were then transferred to air-tight containers in the refrigerator at 4°C until administration. Twenty (20) male albino rats of 100-150g were obtained from the animal house of the Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Nigeria. All experiments were conducted in compliance with ethical guide for care and use of laboratory animals of the Faculty of Pure and Applied Sciences, Federal University Wukari, Nigeria. Keywords Animals specimen Experimental designThe rats were randomly divided into four groups (n = 5) and the extract was administered to the albino rats orally with the aid of oral cannula. e. After the experimental period, animals were sacrificed and venous blood was collected by cardiac puncture and their liver was harvested. Blood samples were collected into EDTA tubes for the plasma and plain sample tubes containing no anticoagulant for the serum. The blood samples were allowed to clot and the serum was obtained by centrifuging at 3,000 rpm for 5 min. The tissues were weighed and homogenized using a standard laboratory mortar and pestle. The homogenates were centrifuged and the supernatant examined for Thiobarbituric acid reactive substance (TBARS). Tissue preparationWeighed liver and kidney samples were homogenized separately in 10 parts (w/v) of ice-cold 50mM Tris-HCl, (pH 7.4) using a homogenizer (Janke and Kunkel Germany). The homogenates were centrifuged at 3,000 rpm for 15...