2009
DOI: 10.1186/1471-230x-9-81
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Liver fibrosis secondary to bile duct injury: correlation of Smad7 with TGF-β and extracellular matrix proteins

Abstract: BackgroundLiver fibrosis is the result of continuous liver injury stemming from different etiological factors. Bile duct injury induces an altered expression of TGF-β, which has an important role in liver fibrosis because this cytokine induces the expression of target genes such as collagens, PAI-1, TIMPs, and others that lead to extracellular matrix deposition. Smad7 is the principal inhibitor that regulates the target gene transcription of the TGF-β signaling. The aim of the study was to determine whether Sm… Show more

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Cited by 11 publications
(7 citation statements)
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References 36 publications
(34 reference statements)
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“…TGF-β is a multifunctional cytokine involved in regulation, differentiation, and proliferation of ECM. TGB-β also has an important role in hepatic fibrogenesis since it activates the over expression of ECM deposits through molecules like PAI-1, TIMPs, collagens, tenascin, fibronectin, and Smad7, as previously reported by our group [25]. In the present study, plasma levels of PAI-1 were higher in BDI compared to healthy controls, which could explain the activation and facilitation of the fibrous tissue deposition in the liver with BDI as well as the very important role PAI-1 has in inhibiting the activation of MMP-3 upon favouring activation of TIMPs [26].…”
Section: Discussionsupporting
confidence: 67%
“…TGF-β is a multifunctional cytokine involved in regulation, differentiation, and proliferation of ECM. TGB-β also has an important role in hepatic fibrogenesis since it activates the over expression of ECM deposits through molecules like PAI-1, TIMPs, collagens, tenascin, fibronectin, and Smad7, as previously reported by our group [25]. In the present study, plasma levels of PAI-1 were higher in BDI compared to healthy controls, which could explain the activation and facilitation of the fibrous tissue deposition in the liver with BDI as well as the very important role PAI-1 has in inhibiting the activation of MMP-3 upon favouring activation of TIMPs [26].…”
Section: Discussionsupporting
confidence: 67%
“…In physiological situations, its increase decreases the phosphorylation of Smad2/3, and thus decreases TGF- β functions. In chronic hepatic injury, the expression of Smad7 is paradoxically decreased [38]; as a result, TGF- β signal transduction cannot be effectively inhibited, and TGF- β functions are enhanced. An aberrant expression of Smad7 may thus disrupt the balanced activity of TGF- β under pathophysiological conditions.…”
Section: Discussionmentioning
confidence: 99%
“…The Videocount Area Array option of the software was also utilized (defined as the number of pixels in a field that met a user-defined color threshold of staining). The number of thresholded blue pixels in each field were then counted and analyzed as a percentage of the total number of pixels in that field, using similar methods as previously described for liver fibrosis (del Pilar Alatorre-Carranza, Miranda-Diaz et al 2009). For the glabrous region of dermis of the mid forepaw, 3 to 4 fields were counted per animal (in cross-sectional tissue slices) using a 20X objective, and the % area of blue staining (fibrous collagen) was averaged for each animal, before comparing results across groups.…”
Section: Methodsmentioning
confidence: 99%