Liver ethoxyresorufin-O-deethylase and brain acetylcholinesterase in two freshwater fish species of South America; the effects of seasonal variability on study design for biomonitoring

Chiang, Gustavo; Munkittrick, Kelly R.; Urrutia, Roberto; Concha, Cesiah; Rivas, Meyling; Díaz-Jaramillo, Mauricio; Barra, Ricardo

doi:10.1016/j.ecoenv.2012.09.008

Cited by 17 publications

(10 citation statements)

References 49 publications

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“…Other similar indications of impacts using EROD activity as biomarker of xenobiotics have been recorded elsewhere (e.g. Chiang et al, 2012;Karimzadeh & Zahmatkesh, 2013;Kammann et al, 2014;Franco-Bernardes, Maschio, Azeredo-Oliveira, & Almeida, 2015).…”

Section: Resultssupporting

confidence: 73%

<b>Induced CYP1A activity and DNA damage in fish from the middle Paraíba do Sul River basin, southeastern Brazil

Morado

Martins

Araújo

et al. 2018

Acta Sci. Biol. Sci.

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The Paraíba do Sul River (PSR) drainage basin in Southeastern Brazil covers one of the most industrialized and densely populated regions of the country. The impact of chemical contamination on the PSR basin seems to be more pronounced in its middle reach where a number of potentially polluting plants are located. In this study, we used hepatic EROD activity-a biomarker of exposure to CYP1A-inducing pollutants (e.g. PAHs, PCDD/Fs, PCBs)-and the incidence of micronucleated erythrocytes (Mn) in the peripheral blood-a biomarker of effects of DNA-damaging agents (e.g. PAHs)to evaluate the effects of pollution on two native fish species, Geophagus brasiliensis and Pimelodus maculatus. Results showed that the incidence of Mn and EROD in G. brasiliensis and P. maculatus from the two most downstream sites (Três Rios Town and Piabanha River) were markedly higher than the incidence of Mn and EROD recorded in fish from the most upstream site (Funil Reservoir). Our findings are consistent with the view that CYP1A-inducing activity and increased DNA-damage are found in fish caught in sampling sites located downstream of the stretch where there are several industries that are potential sources of PAHs and CYP1A-inducing contaminants.

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Section: Resultssupporting

confidence: 73%

<b>Induced CYP1A activity and DNA damage in fish from the middle Paraíba do Sul River basin, southeastern Brazil

Morado

Martins

Araújo

et al. 2018

Acta Sci. Biol. Sci.

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“…( 11 ) and Chiang et al. ( 66 , 67 ) coincide, indicating the spawning season (spring to early summer). T. areolatus has a benthic and benthophagous life strategy, with a closer contact with the sediment, an environmental matrix widely described as the main reservoir of lipophilic compounds.…”

Section: Evidence Of Endocrine Disruption In Fish Native To Chilementioning

confidence: 73%

“…It is necessary to understand that fish are constantly subjected to environmental stress (21,22) before planning EDC effect studies, including changes in temperature, hypoxia, sediment loads, flow rate, among others. To understand and to have the ability to extrapolate the effects observed in the individual organism with a chemical or mixture of chemicals having endocrine disruptive properties, cellular and physiological seasonal variations associated with changes in metabolic rates and fish reproductive status must be taken into consideration when evaluating the impacts on individuals and populations (9,21,33,(66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)80).…”

Section: Discussionmentioning

confidence: 99%

Endocrine Disruptor Impacts on Fish From Chile: The Influence of Wastewaters

et al. 2021

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Industrial wastewaters and urban discharges contain complex mixtures of chemicals capable of impacting reproductive performance in freshwater fish, called endocrine-disrupting compounds (EDCs). In Chile, the issue was highlighted by our group beginning over 15 years ago, by analyzing the impacts of pulp and paper mill effluents (PPME) in the Biobio, Itata, and Cruces River basins. All of the rivers studied are important freshwater ecosystems located in the Mediterranean region of Central Chile, each with a unique fish biodiversity. Sequentially, we developed a strategy based on laboratory assays, semicontrolled-field experiments (e.g., caging) and wild fish population assessments to explore the issue of reproductive impacts on both introduced and native fish in Chile. The integration of watershed, field, and laboratory studies was effective at understanding the endocrine responses in Chilean freshwater systems. The studies demonstrated that regardless of the type of treatment, pulp mill effluents can contain compounds capable of impacting endocrine systems. Urban wastewater treatment plant effluents (WWTP) were also investigated using the same integrated strategy. Although not directly compared, PPME and WWTP effluent seem to cause similar estrogenic effects in fish after waterborne exposure, with differing intensities. This body of work underscores the urgent need for further studies on the basic biology of Chilean native fish species, and an improved understanding on reproductive development and variability across Chilean ecosystems. The lack of knowledge of the ontogeny of Chilean fish, especially maturation and sexual development, with an emphasis on associated habitats and landscapes, are impediment factors for their conservation and protection against the threat of EDCs. The assessment of effects on native species in the receiving environment is critical for supporting and designing protective regulations and remediation strategies, and for conserving the unique Chilean fish biodiversity.

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“…As a result, T. areolatus displays an intricate integration with its environment; yet the species is fairly distributed throughout the region, providing an opportunity for its use as a model sentinel organism to study environmental stress in Chile's freshwater streams 6 , 7 . As a benthic fish, T. areolatus displays interactions with riverine sediments and substrates 8 . This species tends toward a generalist eating strategy, eating aquatic insects 9 as well as the surface organisms present on stream, plant, and rock surfaces 10 ; consequently, its food preferences are known to vary by season 4 .…”

Section: Introductionmentioning

confidence: 99%

“…There are limited transcriptomic resources available for fish native to South America, and none available of the Trichomycterids. Current molecular research with this organism is limited, and includes a karyotype 15 , seasonal variation in biomarkers 8 , local industrial discharge impacts 6 , agricultural disturbances 16 , microsatellite loci for conservation resources 17 , and population genetics 18 .…”

Section: Introductionmentioning

confidence: 99%

De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome

et al. 2016

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Trichomycterus areolatus is an endemic species of pencil catfish that inhabits the riffles and rapids of many freshwater ecosystems of Chile. Despite its unique adaptation to Chile's high gradient watersheds and therefore potential application in the investigation of ecosystem integrity and environmental contamination, relatively little is known regarding the molecular biology of this environmental sentinel. Here, we detail the assembly of the Trichomycterus areolatus transcriptome, a molecular resource for the study of this organism and its molecular response to the environment. RNA-Seq reads were obtained by next-generation sequencing with an Illumina® platform and processed using PRINSEQ. The transcriptome assembly was performed using TRINITY assembler. Transcriptome validation was performed by functional characterization with KOG, KEGG, and GO analyses. Additionally, differential expression analysis highlights sex-specific expression patterns, and a list of endocrine and oxidative stress related transcripts are included.

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Liver ethoxyresorufin-O-deethylase and brain acetylcholinesterase in two freshwater fish species of South America; the effects of seasonal variability on study design for biomonitoring

Cited by 17 publications

References 49 publications

<b>Induced CYP1A activity and DNA damage in fish from the middle Paraíba do Sul River basin, southeastern Brazil

<b>Induced CYP1A activity and DNA damage in fish from the middle Paraíba do Sul River basin, southeastern Brazil

Endocrine Disruptor Impacts on Fish From Chile: The Influence of Wastewaters

De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome

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