The aim of this study was to compare the health status and prognosis of buffalo calves with retention of urine either with intact or ruptured bladder by studying the panel of biochemistry and hematology variables. Blood specimens were collected from male buffalo calves (n=30), which were diagnosed with urine retention either with intact or ruptured urinary bladder on the basis of clinical signs and ultrasonogarphic examination besides control animals (n=15) to perform the different biochemical and hematological tests. Anorexia, depression and abdominal distension were the obvious clinical signs in the affected calves. Ultrasonographically, an anechoic distended structure representing the urinary bladder revealed urine retention with an intact bladder while the presence of free anechoic fluid in the abdominal cavity indicates urine retention with ruptured bladder. Laboratory findings in both the affected groups revealed an increase in serum creatinine, urea and bilirubin (total, direct and indirect) concentrations and aspartate aminotransferase and alkaline phosphatase activities, hyponatremia, hypochloremia, hypocalcemia, hyperphosphatemia, hypomagnesemia, hyperproteinemia, hypoalbuminemia, hyperglobulinemia,relative polycythemia, leukopenia, neutrophilia, lymphopenia, monocytosis and eosinophilia in comparison to the control group, almost of these analytes were changed sharply in ruptured urinary bladder cases in comparison with intact urinary bladder cases. Hyperkalemia was observed in the ruptured bladder group only. In conclusion, buffalo calves which suffered from urine retention with ruptured bladder have severe alterations in most of biochemical and hematological analytes in comparison with the intact bladder group and some of these cases may die from severe uraemia and electrolytes disturbance (poor prognosis).