Liver alcohol dehydrogenase activity and ethanol levels during chronic ethanol intake in pregnant rats and their offspring

The effects of pregnancy on ethanol clearance

“…However, Litvin and Switzer (1988) have reported lower rates of ethanol elimination in pregnancy in guinea pigs. In addition, ethanol elimination did not differ and decreased hepatic ADH activity at term was reported in one study of pregnant rats fed ethanol compared to controls (Traves et al, 1995). In contrast, although no effects were observed on hepatic ADH activity, significant reductions in mean BECs in pregnant rats were reported following acute ethanol injection i.p.…”

GOTPUREBLOOD IN FETAL RATS?

“…To study malnutrition-induced IUGR, it is reasonable to measure fetal blood glucose levels; to study hypoxia-induced IUGR, it is rational to determine fetal blood gases. Unfortunately, previous studies were performed either without measuring fetal blood values due to methodological limitation, or using samples collected from decapitated rat fetuses [1][2][3].…”

“…In almost all previous fetal physiological and functional experiments in rats, fetal blood collection in rats depends on decapitation [2,3]. The "blood" collected this way is mixed with arterial and vein blood, as well as tissue fluids.…”

Gamnamoside, a Phenylpropanoid Glycoside from Persimmon Leaves (Diospyros kaki) with an Inhibitory Effect against an Alcohol Metabolizing Enzyme

“…ADH activity was measured according to the method in the literature with a modification. 17,18 The test solutions were prepared by dissolving the isolates in DMSO and were subsequently diluted with the sodium phosphate buffer solution (10 mM, pH 7.4) by a two-fold serial dilution method. All the following solutions were prepared in a sodium phosphate buffer solution (10 mM, pH 7.4).…”