Live visualizations of single isolated tubulin protein self-assembly via tunneling current: effect of electromagnetic pumping during spontaneous growth of microtubule

Abstract

As we bring tubulin protein molecules one by one into the vicinity, they self-assemble and entire event we capture live via quantum tunneling. We observe how these molecules form a linear chain and then chains self-assemble into 2D sheet, an essential for microtubule, —fundamental nano-tube in a cellular life form. Even without using GTP, or any chemical reaction, but applying particular ac signal using specially designed antenna around atomic sharp tip we could carry out the self-assembly, however, if there is no electromagnetic pumping, no self-assembly is observed. In order to verify this atomic scale observation, we have built an artificial cell-like environment with nano-scale engineering and repeated spontaneous growth of tubulin protein to its complex with and without electromagnetic signal. We used 64 combinations of plant, animal and fungi tubulins and several doping molecules used as drug, and repeatedly observed that the long reported common frequency region where protein folds mechanically and its structures vibrate electromagnetically. Under pumping, the growth process exhibits a unique organized behavior unprecedented otherwise. Thus, “common frequency point” is proposed as a tool to regulate protein complex related diseases in the future.

Seeing Cell Biology with the Eyes of Physics

“…The introduction of the issue of protein conductivity in RRM development, led to the consideration that a charge moving through the protein backbone would generate an electromagnetic irradiation or absorption with spectral signatures corresponding to energy distribution along the protein [19,20]. These theoretically calculated spectra were confirmed experimentally [11,21]. Moreover, RRM allows designing of new peptides with desired spectral characteristics, and biological activities [22].…”

“…Rather, the intracrine world may even contribute to the deployment of an "intracellular niche", a complex nanotopography where signaling molecules interplay with a network of filaments and microtubuli which are far away from simply being the cytoskeleton. Scanning tunneling Microscopy (STM), coupled with a special artificial cell-like environment designed to pump electromagnetic frequencies to microtubuli growing onto a nanoelectrode array, has shown the existence of defined resonance modes between the tubulin protein, as well as the microtubular structures, and the applied frequencies [11]. Moreover, STM showed that specific "tunneling current images" are produced by microtubuli as resonance patterns in response to electromagnetic frequencies in the MHz domain [11].…”

Encouraging Submission of Studies Seeing Stem Cell Biology in the Light of Physical Energies and Nanoscale Approaches: Towards a Regenerative Medicine without the Needs for Stem Cell Transplantation

“…Similarly, STM analyses have shown that single microtubule tunneling "current images" are produced when different resonance frequencies are pumped simultaneously [3]. The frequency region selectivity for engaging particular kinds of conformational changes establishes that pure mechanical changes can be remotely controlled in an atomically precise fashion by using electromagnetic fields remotely.…”

“…Using artificial cell-like environment that works as a cavity (a replica of a living cell), within an ad hoc designed setting capable of pumping electromagnetic frequencies to a growing microtubule, in conjunction with scanning tunneling microscopy (STM), it has been possible to detect how different frequencies change the local density of states in the tubulin protein structure [3]. Similarly, STM analyses have shown that single microtubule tunneling "current images" are produced when different resonance frequencies are pumped simultaneously [3].…”

“…Compelling evidence demonstrate that our cells are able to generate electromagnetic signals [1][2][3][4][5] and mechanical oscillations [6][7][8][9], affording essential modulation of cell polarity.…”