Live transportation of Macrobrachium rosenbergii (De Man) in chilled sawdust

Abstract

Three cooling rates of 1.26 AE 0.09 1C h À1 within 8 h (slow,T 1 ), 2.52 AE 0.18 1C h À1 within 4 h (moderate,T 2 ) and 5.04 AE 0.36 1C h À1 within 2 h (fast, T 3 ) were tested to cold-anaesthetize farm raised Macrobrachium rosenbergii (De Man) (45^52 g) in each case from 25 1C down to 15 AE 1 1C in a refrigerated chilling tank, provided with aeration. The cold-anaesthetized prawns subjected to each chilling rate were packed in an insulated cardboard box (triplicate) between two layers of moist and chilled (2^3 1C) sawdust, and kept inside a chilled storage cabinet at 15 AE 1 1C, for set durations of 6, 9, 12, 15 and 18 h. Survival was determined by revitalizing the prawns in aerated water with an initial temperature of 20 1C, which was raised to 29 AE 1 1C within 3 h. The experiment was repeated using berried females acclimated to brackishwater of 12 g L À 1 salinity and the percentage survival recorded after live storage for durations ranging from 6 to 24 h at intervals of 3 h. Statistically valid safe durations for obtaining100% survival of the cold anaesthetized and live stored prawns were determined using probit analysis at the three chilling rates tested, and were found to be 7.39, 6.98 and 4.54 h in the case of adult prawns, and 7.87, 8.17 and 6.43 h for berried females for T 1 , T 2 and T 3 respectively. For practical purposes, the durations that yielded 95% survival rates were computed to be 16. 47, 12.14 and 8.35 h in the case of adult prawns and 18.49, 19.02 and11.11h for berried females forT 1 ,T 2 , andT 3 respectively. The berried prawns revitalized after live storage were incubated in tanks and the zoea larvae reared up to postlarvae (PL-5), and compared against a control. No signi¢cant di¡erence was found in larval hatch fecundity, survival rate and the production of PL L À 1 between the treatment and control, indi-cating that the method of cold anaesthetization and live storage of berried prawns could be used for successful transportation of broodstock.

Anaesthetic Effects of Eugenol on Grass Shrimp (Palaemonetes sinensis) of Different Sizes at Different Concentrations and Temperatures

“…Eugenol is easily obtained and is generally considered safe by most national authorities. It has also proved effective in many crustaceans, including Homarus americanus 12 , Fenneropenaeus indicus 20 , Macrobrachium rosenbergii 1 , and Nephrops norvegicus 21 . In the present study, eugenol was found to be effective for anaesthetising P. sinensis .…”

“…This will be useful in commercial applications such as handling, netting, and long-distance live transport, which are difficult and expensive for many crustacean species 19,20 . The results of this experiment can be used to determine the effectiveness of different concentrations for different size classes during the capture and transport process from wild environments.…”

Health management during handling and live transport of crustaceans: A review

“…Macrobrachium rosenbergii Coyle et al (2001Coyle et al ( , 2005, Tidwell and Coyle (2002), Salin (2005), Tidwell et al (2005), www.isuagcentre.com and www.msucares.com Prawns (marine)…”

Survival prediction system for waterless live Chinese Sturgeon transportation based on temperature related glucose changes

“…Live fish are particularly favorite in China where aquariums and tanks displaying live fish in restaurants, supermarkets, and retail outlets are becoming increasingly common compared with fresh frozen product (Mi, Qian, & Mao, ; Salin, ). In recent years, consumers developed important health related attitudes which lead to increased demands for live fish products.…”