Live recombinant Lactococcus lactis vaccine expressing immobilization antigen (i-Ag) for protection against Ichthyophthirius multifiliis in goldfish

Yao, Jiayun; Yuan, Xuemei; Xu, Yongping; Yin, Wu; Lin, Lingyun; X, Pan; Yang, Guilian; Wang, Chunfeng; Shen, Jinyu

doi:10.1016/j.fsi.2016.09.037

Cited by 18 publications

(18 citation statements)

References 38 publications

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“…A live recombinant Lactococcus lactis vaccine expressing a 48 kDa immobilization antigen has been tested in goldfish. The recombinant vaccine was administered through feed and, even though Iag-specific antibody levels were detected, only a limited effect was observed on survival of the fish (50-60 % compared to 40 % in controls) [75].…”

Section: Vaccinesmentioning

confidence: 99%

The fish parasite Ichthyophthirius multifiliis – Host immunology, vaccines and novel treatments

Jørgensen

2017

Fish & Shellfish Immunology

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Ichthyophthirius multifiliis, the causative agent of white spot disease (ichthyophthiriasis) is a major burden for fish farmers and aquarists globally. The parasite infects the skin and the gills of freshwater fish, which may acquire a protective adaptive immune response against this disease, making vaccine strategies feasible. However, there is no prophylactic treatment available and repetitive treatments with auxiliary substances are needed to control the infection. Historically, a variety of drugs and chemicals have been used to combat the disease but due to changing regulations and recognition of carcinogenic and environmentally damaging effects the most efficient compounds are prohibited. A continuous search for novel substances, which are highly effective against the parasites and harmless for the fish is ongoing. These compounds should be environmentally friendly and cost-effective. This review presents recent progress within host immunology, vaccinology and a description of novel substances, which have been tested as treatments against ichthyophthiriasis.

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Section: Vaccinesmentioning

confidence: 99%

The fish parasite Ichthyophthirius multifiliis – Host immunology, vaccines and novel treatments

Jørgensen

2017

Fish & Shellfish Immunology

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“…The bacteria Lactococcus lactis Lister, 1873 was observed to have potential as a delivery vehicle for the oral vaccination of goldfish, Carassius auratus L., against I. multifiliis (see Yao et al . , 2016). Immobilized theronts of I. multifiliis and C. irritans have also been used for the immunization of fish against these ciliates (Alishahi & Buchmann, 2006; Dan et al .…”

Section: Current Management Strategies To Control Ciliate Protozoan Pmentioning

confidence: 99%

“…Bacteria and/or the compounds produced by them can, therefore, be used in the biocontrol of ciliates (details provided in Table 3). Some attempts have also been made related to vaccine development against ciliate protozoan parasites (Alishahi & Buchmann, 2006;Luo et al, 2007;Yao et al, 2016;Watanabe et al, 2019). The bacteria Lactococcus lactis Lister, 1873 was observed to have potential as a delivery vehicle for the oral vaccination of goldfish, Carassius auratus L., against I. multifiliis (see Yao et al, 2016).…”

Section: Parasitic Fish Ciliates and Water Quality Conditions In Warmmentioning

confidence: 99%

“…Some attempts have also been made related to vaccine development against ciliate protozoan parasites (Alishahi & Buchmann, 2006;Luo et al, 2007;Yao et al, 2016;Watanabe et al, 2019). The bacteria Lactococcus lactis Lister, 1873 was observed to have potential as a delivery vehicle for the oral vaccination of goldfish, Carassius auratus L., against I. multifiliis (see Yao et al, 2016). Immobilized theronts of I. multifiliis and C. irritans have also been used for the immunization of fish against these ciliates (Alishahi & Buchmann, 2006;Dan et al, 2013).…”

Section: Parasitic Fish Ciliates and Water Quality Conditions In Warmmentioning

confidence: 99%

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Unveiling associations between ciliate parasites and bacterial microbiomes under warm‐water fish farm conditions – a review

Jahangiri

Shinn

Pratoomyot

et al. 2020

Reviews in Aquaculture

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Intensive fish production has arguably resulted in a consequential increase in the incidence of disease within the aquaculture industry. The frequency and the severity of disease events are in part affected by changing global climatic conditions. More importantly, these changes directly influence changes in water quality parameters and the dynamics of a microbiome, pathogenic or not, in aquatic farming. Changes in temperature, for example, have both direct and indirect effects on the health status of fish, potentially increasing their predisposition to infections. In general terms, fish infections are rarely caused by a single agent or factor – frequently, multiple pathogens, changes in water quality parameters, production factors (e.g. feed quality, stocking density) and host immunity are concomitantly responsible for the establishment of infections in fish farms. Ciliate protozoans represent an important microfauna in aquatic environments. Ciliated protozoans can proliferate quickly, debilitating the host, and, in the absence of an intervention, can lead to a significant loss of stock. Ciliate infections frequently facilitate the establishment of secondary microbial species, both of which are linked to environmental and culture conditions. In this review, economically important ciliated protozoans of warm‐water cultured fish and their potential interactions with their associated bacterial microbiomes (both on/in their hosts and their surrounding environment) are discussed. Also, potential environmental triggers behind ciliate outbreaks within warm‐water aquaculture are reviewed. With better knowledge about such interactions, it will be possible to highlight innovative strategies to control ciliate parasites and bacterial infections in aquaculture.

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“…In recent years, many researchers have demonstrated the antiparasitic activities of extracellular metabolites of microorganisms such as Edwardsiella ictaluri (Xu, Shoemaker, Martins, Pridgeon, & Klesius, ), Streptomyces griseus SDX‐4 (Yao et al., , ), Streptomyces sp. HL‐2‐14 (Gao et al., ), recombinant Lactococcus lactis (Yao et al., ) and Pseudomonas H6 (Al‐Jubury et al., ) against the protozoan I. multifiliis . Studies of the antiparasitic effect of I. multifiliis by plant metabolites return to the work of Buchmann, Jensen, and Kruse () and Ekanem, Obiekezie, Kloas, and Knopf (), who investigated the antiprotozoal activity of crude extracts of garlic ( Allium sativum ), Mucuna pruriens (Fabaceae) and Carica papaya (Caricaceae).…”

Section: Introductionmentioning

confidence: 99%

The in vitro and in vivo effect of tannic acid on Ichthyophthirius multifiliis in zebrafish (Danio rerio) to treat ichthyophthiriasis

Alavinia

Mirzargar

Rahmati‐Holasoo

et al. 2018

Journal of Fish Diseases

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The in vitro antiparasitic effect of polyphenol tannic acid (TA) on Ichthyophthirius multifiliis theronts and tomonts was evaluated. In vitro antiparasitic assays revealed that TA in a dose- and time-dependent pattern through the damage of parasite plasma membrane could be 100% effective against I. multifiliis theronts at concentrations of 8 and 11 ppm during all the exposure times (45-270 min). The tomonts proliferation was completely inhibited by penetrating TA (at least 15 ppm for 22-hr exposure) into encysted tomont across the cyst wall. However, 10 ppm TA could result in a ninefold decrease in the population of live tomonts compared to the control group (p < 0.05). Although at theront concentrations of over 6,000 per zebrafish (Danio rerio), a 100% prevalence of ichthyophthiriasis during a 5-day exposure was recorded, results of in vivo tests showed that the parasite that pretreated up to 10 ppm TA for 70 min had not any capability to infect the studied zebrafish population. The acute toxicity (96 hr-LC ) of TA for zebrafish was 19.51 ppm. Thus, TA can be considered as a natural therapeutant to safely and efficiently improve the health of aquatic systems by controlling ichthyophthiriasis.

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Live recombinant Lactococcus lactis vaccine expressing immobilization antigen (i-Ag) for protection against Ichthyophthirius multifiliis in goldfish

Cited by 18 publications

References 38 publications

The fish parasite Ichthyophthirius multifiliis – Host immunology, vaccines and novel treatments

The fish parasite Ichthyophthirius multifiliis – Host immunology, vaccines and novel treatments

Unveiling associations between ciliate parasites and bacterial microbiomes under warm‐water fish farm conditions – a review

The in vitro and in vivo effect of tannic acid on Ichthyophthirius multifiliis in zebrafish (Danio rerio) to treat ichthyophthiriasis

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