Live foals produced from sperm-injected oocytes derived from pregnant mares

Cochran, R.; Meintjes, M.; Reggio, B.C.; Hylan, D.; Carter, J.; Pinto, C. R. F.; Paccamonti, D.; Godke, R.A.

doi:10.1016/s0737-0806(98)80504-2

Cited by 100 publications

(47 citation statements)

References 31 publications

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“…Normal live offspring derived from ICSI have been reported in several mammalian species including mice [35], horses [36], cattle [37] and humans [38]. However, information on rat ICSI is very limited.…”

Section: Intracytoplasmic Sperm Injection (Icsi)mentioning

confidence: 99%

Maintenance of Transgenes in Rats: The Contributions of Embryo Cryopreservation and Intracytoplasmic Sperm/Spermatid Injection.

Hochi

Hirabayashi

2002

J. Reprod. Dev.

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Abstract. The maintenance of transgenes in rats is reviewed with special references to embryo cryopreservation (transgene banking) and intracytoplasmic injection of spermatozoa or round spermatids (transgene passage). Production of transgenic rats by pronuclear DNA microinjection is easily performed, particularly using the micro-vibration method. Two-cell stage embryos derived from the mating of transgenic rats with wild-type rats can be successfully cryopreserved by conventional two-step freezing in the presence of 10% dimethylsulfoxide. However, the transmission rates of transgenes into newborn offspring are lower than the 50% which is expected in simple Mendelian inheritance. Furthermore, some transgenic rats are unable to transmit their transgenes to subsequent generations. When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques such as intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) can be used for passage of the transgenes. Our ICSI procedure includes the use of a piezo-driven small-sized injection pipette (2-4 µm in diameter), isolated sperm heads, and freshly ovulated oocytes. In the case of ROSI, round spermatids are injected into Sr 2+ -activated oocytes using a piezo-driven middle-sized pipette (5-6 µm in diameter). These microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes.

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Section: Intracytoplasmic Sperm Injection (Icsi)mentioning

confidence: 99%

Maintenance of Transgenes in Rats: The Contributions of Embryo Cryopreservation and Intracytoplasmic Sperm/Spermatid Injection.

Hochi

Hirabayashi

2002

J. Reprod. Dev.

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“…In large animals, the birth of live offspring after ICSI has been reported (pigs (Kolbe & Holtz 2000, Martin 2000, cattle (Goto et al 1990); sheep (Catt et al 1996), and horses (Cochran et al 1998)), although it is well known that developmental success in these species remains remarkably low after this technique. In natural fertilization, oocytes penetrated by spermatozoa are activated normally (as evidenced by extrusion of the second polar body and formation of the male and female pronuclei), but the efficiency of oocyte activation after ICSI is quite low unless the oocytes further receive artificial stimulation (Fujinami et al 2004, Nakai et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Pre-treatment of sperm reduces success of ICSI in the pig

Nakai¹,

Ito²,

Sato³

et al. 2011

Reproduction

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In pigs, although ICSI is a feasible fertilization technique, its efficiency is low. In general, injected pig sperm are insufficient to induce oocyte activation and embryonic development. Pretreatments for disrupting sperm membranes have been applied to improve the fertility of ICSI oocytes; however, we hypothesize that such pretreatment(s) may reduce the ability of the sperm to induce oocyte activation. We first evaluated the effects of sperm pretreatments (sonication (SO) to isolate the sperm heads from the tails, Triton X-100 (TX), and three cycles of repeated freezing/thawing (3!-FT) for disrupting sperm membranes) on the rate of pronucleus (PN) formation after ICSI. We found that oocytes injected with control (whole) sperm had higher rates of PN formation than those obtained after subjecting the sperm to SO, TX, and 3!-FT. The amounts of phospholipase Cz (PLCz), which is thought to be the oocyte-activating factor in mammalian sperm, in sperm treated by each method was significantly lower than that in whole untreated sperm. Furthermore, using immunofluorescence, it was found that in pig sperm, PLCz was localized to both the post-acrosomal region and the tail area. Thus we demonstrated for the first time that sperm pretreatment leads to a reduction of oocyte-activating capacity. Our data also show that in addition to its expected localization to the sperm head, PLCz is also localized in the tail of pig sperm, thus raising the possibility that injection of whole sperm may be required to attain successful activation in pigs.

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“…This group later performed ICSI at room temperature (23 -25 8C) without a holding period and obtained similar survival rates (Wakayama & Yanagimachi 1998a,b). In the horse, some researchers use a heated stage for conventional ICSI (Dell'Aquila et al 1997a,b, 2001, Cochran et al 1998), but there is no information available on the effect of manipulation temperature on the survival or early development of equine embryos.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection

Choi

Love²,

Varner³

et al. 2004

Reproduction

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This study was conducted to evaluate the effect of initial cumulus morphology (expanded or compact) and duration of in vitro maturation (24, 30 or 42 h) on the developmental competence of equine oocytes after intracytoplasmic sperm injection (ICSI). The effect of manipulation temperature (room temperature vs 37 8C) at the time of ICSI and concentration of glucose (0.55 vs 5.5 mM) during embryo culture was also investigated. The nuclear maturation rates of expanded (Ex) oocytes were significantly (P < 0.001) higher than those of compact (Cp) oocytes at all maturation times (61-72 vs 23 -25% respectively). Forty-eight hours after ICSI of mature Ex oocytes, the rate of cleavage with normal nuclei was significantly (P < 0.05) higher for oocytes matured for 24 h than for those matured for 30 or 42 h (73 vs 57-59% respectively). For Cp oocytes, the morphologic cleavage rates for oocytes matured for 30 h were significantly higher (P < 0.05) than for those matured for 24 or 42 h (86 vs 55-61% respectively). The overall proportion of embryos having more than four normal nuclei at 48 h culture was significantly higher (P < 0.05) for Cp than for Ex oocytes. Manipulation temperature did not affect development of embryos from Ex or Cp oocytes at 96 h after ICSI. Culture in high-glucose medium significantly increased morphologic cleavage of Cp, but not Ex, oocytes (P < 0.05). Embryos from Cp oocytes had a significantly higher average nucleus number after 96-h culture than did embryos from Ex oocytes. These data indicate that developmental competence differs between Ex and Cp equine oocytes, and is differentially affected by the duration of maturation and by composition of embryo culture media.

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Live foals produced from sperm-injected oocytes derived from pregnant mares

Cited by 100 publications

References 31 publications

Maintenance of Transgenes in Rats: The Contributions of Embryo Cryopreservation and Intracytoplasmic Sperm/Spermatid Injection.

Maintenance of Transgenes in Rats: The Contributions of Embryo Cryopreservation and Intracytoplasmic Sperm/Spermatid Injection.

Pre-treatment of sperm reduces success of ICSI in the pig

Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection

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