2012
DOI: 10.1002/cbic.201200381
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Live‐Cell Studies of p300/CBP Histone Acetyltransferase Activity and Inhibition

Abstract: Histone acetyltransferase enzymes (HATs) are important therapeutic targets, but there are few cell-based assays available for evaluating the pharmacodynamics of HAT inhibitors. Here we present the application of a FRET-based reporter, Histac, in live cell studies of p300/CBP HAT inhibition, by both genetic and pharmacologic disruption. shRNA knockdown of p300/CBP led to increased Histac FRET, suggesting a role for p300/CBP in the acetylation of the histone H4 tail present. Additionally, we describe a new p300/… Show more

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Cited by 47 publications
(57 citation statements)
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References 46 publications
(24 reference statements)
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“…To account for potential off-target effects of C646 we used C37 as a control, a compound very similar in structure to C646 but that shows no effect on CBP HAT activity (Bowers et al, 2010). C646 and related inhibitors have previously been shown to affect histone acetylation in Drosophila and mammalian cells within minutes (Crump et al, 2011; Dancy et al, 2012). To assess the immediate transcriptional response to CBP inhibition genome-wide, we performed precision run-on sequencing (PRO-seq) with S2 cells treated with C37 (control) or C646 for 10 min.…”
Section: Resultsmentioning
confidence: 99%
“…To account for potential off-target effects of C646 we used C37 as a control, a compound very similar in structure to C646 but that shows no effect on CBP HAT activity (Bowers et al, 2010). C646 and related inhibitors have previously been shown to affect histone acetylation in Drosophila and mammalian cells within minutes (Crump et al, 2011; Dancy et al, 2012). To assess the immediate transcriptional response to CBP inhibition genome-wide, we performed precision run-on sequencing (PRO-seq) with S2 cells treated with C37 (control) or C646 for 10 min.…”
Section: Resultsmentioning
confidence: 99%
“…Thus, simply inducing a histone hyperacetylated state with HDACi does not adequately replace HAT activation, at least in certain functions (i.e., memory formation), likely reflecting the other potential roles of HAT (e.g., CBP). The indirect (HDAC inhibitor-mediated) activation of p300/ CBP HAT auto-acetylation, which stimulates HAT activity [36,216], could also contribute to the beneficial effects of HDACi. Thus, it seems essential to envisage direct stimulation of the HAT function, as a new therapeutic tool in neurodegenerative diseases; by directly targeting the remaining HAT(s), one could re-activate the deficient enzymatic function, as well as all the other related functions that are not typically considered using an HDACi strategy.…”
Section: Hat Activation Vs Hdac Inhibition As a Therapeutic Strategymentioning
confidence: 99%
“…19 Inhibitors of p300/CBP HAT activity have been developed and are under investigation as therapeutics for a number of diseases. 5,10,14,22,23 Additional key p300/CBP domains include a well-characterized bromodomain that is just N-terminal to the HAT domain and can bind acetyl-Lys-containing peptides. The bromodomain is one of the most highly conserved domains in p300 and CBP, possessing 96% sequence identity.…”
mentioning
confidence: 99%