Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in theCaenorhabditis elegansembryo
Makoto Goda,
Michael Shribak,
Zenki Ikeda
et al.
Abstract:Organelles in cells are appropriately positioned, despite crowding in the cytoplasm. However, our understanding of the force required to move large organelles, such as the nucleus, inside the cytoplasm is limited, in part owing to a lack of accurate methods for measurement. We devised a novel method to apply forces to the nucleus of living, wild-typeCaenorhabditis elegansembryos to measure the force generated inside the cell. We utilized a centrifuge polarizing microscope (CPM) to apply centrifugal force and o… Show more
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