Live cell imaging to understand monocyte, macrophage, and dendritic cell function in atherosclerosis

McArdle, Sara; Mikulski, Zbigniew; Ley, Klaus

doi:10.1083/jcb.2136oia120

Cited by 5 publications

(5 citation statements)

References 78 publications

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“…However, surgery itself might inevitably produce a local, and even a systemic pro‐inflammatory stimulus , which will affect the structure and function of vasculature as well as the recruitment and migration of immunocytes. The footpad without hair follicles provides a classical physiological site for investigating cellular recruitment and motility during inflammations (eg, DTH) because it is nearby popliteal lymph node . In addition, the limited imaging depth restricts the investigations on immunological events to the superficial dermis only , which leads to the information deficiency of immunological events in the relatively deeper dermis.…”

Section: Discussionmentioning

confidence: 99%

“…This fact implies that the intensity of the immune response of MMs may be different depending on the depth in tissue around the inflammatory foci . The footpad without hair follicles provides a classical physiological site for investigating cellular recruitment and motility during inflammations because it is nearby popliteal lymph node . However, the immunological events observed by optical microscopies are usually restricted to the superficial dermis due to the unsatisfactory imaging performance caused by the strong scattering of the footpad skin , which resulted in the information deficiency in the relatively deeper internal tissue.…”

Section: Introductionmentioning

confidence: 99%

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In vivo imaging the motility of monocyte/macrophage during inflammation in diabetic mice

Shi

Feng

Zhang

et al. 2018

Journal of Biophotonics

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Diabetes, as a chronic metabolic disease, can impair the immune function of monocytes/macrophages (MMs). However, it is unclear how MM immune response to inflammation with the development of diabetes, and whether immune response around the inflammatory foci depends on the depth in tissue. Footpad provides a classical physiological site for monitoring cellular behavior during inflammation, but limited to the superficial dermis due to the strong scattering of footpad. Herein, we used confocal microscopy to monitor the motility of MMs in deeper tissue around inflammatory foci with the development of type 1 diabetic (T1D) mice through a switchable footpad skin optical clearing window. Delayed-type hypersensitivity (DTH) model was elicited on the footpad of T1D. Results demonstrated that progressive T1D led to the gradually potentiated MM recruitment and increased expression of monocyte chemoattractant protein-1 during DTH, but MM migration displacement, motion velocity and motility coefficient were significantly attenuated. Besides, MMs from the deeper dermis had a much larger migration displacement than those from superficial dermis at early stages of DTH but an opposite tendency at late stages for non-T1D, while progressive T1D obscured this difference gradually. This study will be helpful for investigating the influences of progressive metabolic diseases on immune response. MM motion trajectory at depth of superficial dermis and the deeper dermis at AOVA (heat-aggregated ovalbumin)-4 hours and AOVA-72 hours on non-T1D (A) and T1D-4 weeks (B). Mean motility coefficient (C) at the 2 depths. Data were pooled from 6 mice per group. *P < .05 and **P < .01 compared among different T1D disease durations. #P < .05 compared between different depths.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vivo imaging the motility of monocyte/macrophage during inflammation in diabetic mice

Shi

Feng

Zhang

et al. 2018

Journal of Biophotonics

View full text Add to dashboard Cite

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“…[12][13][14] However, with current knowledge on MФ origin, diversity and significant plasticity, 15,16 the acceptance of this dogma has diminished. Recent cuttingedge technological developments such as single-cell RNA sequencing (scRNAseq), advanced animal genetic modification and intravital microscopy [17][18][19][20] have allowed for a greater appreciation of cellular diversity than previously acknowledged. MФ not only have impressive variability in their gene expression but they are also phenotypically plastic, allowing them to adapt to their environment and ensure appropriate responses.…”

Section: Introductionmentioning

confidence: 99%

Macrophage reprogramming for therapy

et al. 2021

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Dysfunction of the immune system underlies a plethora of human diseases, requiring the development of immunomodulatory therapeutic intervention. To date, most strategies employed have been focusing on the modification of T lymphocytes, and although remarkable improvement has been obtained, results often fall short of the intended outcome. Recent cutting-edge technologies have highlighted macrophages as potential targets for disease control. Macrophages play central roles in development, homeostasis and host defence, and their dysfunction and dysregulation have been implicated in the onset and pathogenesis of multiple disorders including cancer, neurodegeneration, autoimmunity and metabolic diseases. Recent advancements have led to a greater understanding of macrophage origin, diversity and function, in both health and disease. Over the last few years, a variety of strategies targeting macrophages have been developed and these open new therapeutic opportunities. Here, we review the progress in macrophage reprogramming in various disorders and discuss the potential implications and challenges for macrophage-targeted approaches in human disease.

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“…One drawback of fusion proteins is the possibility to exhibit altered biological function or molecular structure. Thus, careful characterization before FRET is recommended ( Masi et al ., 2010 ; McArdle et al ., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Visualization of integrin molecules by fluorescence imaging and techniques

Cai

Sun

et al. 2021

Biocell

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Integrin molecules are transmembrane αβ heterodimers involved in cell adhesion, trafficking, and signaling. Upon activation, integrins undergo dynamic conformational changes that regulate their affinity to ligands. The physiological functions and activation mechanisms of integrins have been heavily discussed in previous studies and reviews, but the fluorescence imaging techniques -which are powerful tools for biological studies- have not. Here we review the fluorescence labeling methods, imaging techniques, as well as Förster resonance energy transfer assays used to study integrin expression, localization, activation, and functions.

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Live cell imaging to understand monocyte, macrophage, and dendritic cell function in atherosclerosis

Cited by 5 publications

References 78 publications

In vivo imaging the motility of monocyte/macrophage during inflammation in diabetic mice

In vivo imaging the motility of monocyte/macrophage during inflammation in diabetic mice

Macrophage reprogramming for therapy

Visualization of integrin molecules by fluorescence imaging and techniques

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