Live-cell bioorthogonal Raman imaging

Metabolic Remodeling of Cell‐Surface Sialic Acids: Principles, Applications, and Recent Advances

“…Apart from the signal specificity issue, the fact that intrinsic Raman scattering has a sensitivity order of magnitude lower than fluorescence is another challenge for Raman imaging of sialoglycans. We developed a bioorthogonal Raman imaging strategy in which a bioorthogonal Raman reporter is functionalized onto ManNAc or Neu5Ac analogues for direct metabolic incorporation and enhanced Raman imaging techniques are used for improving the imaging sensitivity (Figure A) …”

“…Reprinted and modified, with permission, from refs. , and . Copyright: Elsevier, 2015, and Wiley‐VCH, 2014.…”

“…The bioorthogonal Raman imaging of sialoglycans should provide a complementary means to fluorescence imaging. Notably, this new imaging strategy has also been extended to other biomolecules, including nucleic acids, proteins, and lipids …”

Detection and time-tracking activation of a photosensitiser on live single colorectal cancer cells using Raman spectroscopy

“…13 For tracking a drug within a cell, alternative approaches can be used, such as Resonance Raman scattering (RRS), the use of isotopologues such as deuterium labelling 14,15 or biorthogonal Raman tagging such as through alkyne incorporation. 13,[16][17][18] RRS has been employed to study cytochrome C at 532 nm, 19,20 where a drop in signal intensity, due to oxidation, has been observed at early stages of apoptosis. 19 Biorthogonal Raman tagging uses molecules that exhibit vibrational bands in the Raman silent region of cells (1800-2800 cm −1 ) such as alkynes, enabling more accurate detection of compounds in single cells.…”

Speciation of Cr in waste rocks of Xinqiao pyrite mine in Tongling, China

“…Most of the studies deal with the contamination of waste rocks by heavy metals using only the generation of AMD and the total metal content as a criterion to assess its potential effect as contaminant [2,3,4,5]. And many scholars have done a lot of researches on the modes of occurrence, distribution characters of heavy metals around mine tailing reservoirs [6,7,8,9].…”