Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility

Nagata, Maria Portia B.; Endo, Kenji; Ogata, Kenichi; Yamanaka, Kenichi; Egashira, Junki; Katafuchi, Naoto; Yamanouchi, T.; Matsuda, Hideo; Goto, Yuki; Sakatani, Miki; Hojo, Takuo; Nishizono, Hirofumi; Yotsushima, Kenji; Takenouchi, Naoki; Hashiyada, Y.; Yamashita, Kenichi

doi:10.1073/pnas.1717974115

Cited by 67 publications

(65 citation statements)

References 68 publications

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“…This hypothesis seems to be reasonable for in vivo fertilization, but it is unknown whether it can be applied to IVF because the oocytes are surrounded by abundant sperm during IVF. In addition, there are many reports that several sperm selection methods can improve the fertilization rate or embryo development rate [40,41,42,43,44]. Therefore, it can be assumed that NT affected only a partial population of optimal sperm and contributed to the improvement in early embryo development and blastocyst quality.…”

Section: Discussionmentioning

confidence: 99%

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Umezu

Yajima²,

Hiradate

et al. 2019

Journal of Reproduction and Development

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Previously, we reported that neurotensin (NT), which is expressed in the uterus and oviduct, enhanced bovine sperm capacitation and acrosome reactions. As NT mRNA expression in bovine oviducts increases dramatically in the follicular phase, we hypothesized that NT modulates fertilization and subsequent conception in cattle. The objective of this study was to evaluate the effect of NT on embryo development and blastocyst quality. The rate of embryo cleavage was significantly increased by the addition of NT to the fertilization medium. Furthermore, the total number of cells and numbers of cells in the inner cell mass of blastocysts were significantly increased by NT during in vitro fertilization (IVF). These results suggested that NT enhanced the efficiency of early bovine embryo development and blastocyst quality. The expression of NT receptors (NTRs) in sperm, testes, oocytes, and cumulus cells was evaluated to determine whether NT acted via NTRs in sperm alone or in both male and female reproductive cells during IVF. Immunocytochemistry and reverse transcription polymerase chain reaction revealed that NTR1 and NTR2 were expressed in sperm and testes, but not in oocytes and cumulus cells. We propose that NT selectively acts upon sperm via NTR1 and NTR2 during IVF to improve the cleavage rate and quality of blastocysts, which are important determinants of sperm quality for successful conception. This research supports our hypothesis that NT acts as a key modulator of fertilization and conception in cattle. Further studies are necessary to apply our findings to the industrial framework of bovine reproduction.

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Section: Discussionmentioning

confidence: 99%

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Umezu

Yajima²,

Hiradate

et al. 2019

Journal of Reproduction and Development

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“…Recently, Nagata et al . [ 36 ] sorted bull cryopreserved spermatozoa into two subpopulations by using the microfluidic system. One subpopulation was predominately composed of rapidly linear progressive nonsinuous spermatozoa and the other was predominately composed of relatively slowly transitional sinuous spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

“…From these results, they proposed that relatively slowly transitional sinuous spermatozoa are associated with fertility and may be truly functional cells, rather than the rapid and more linear spermatozoa. However, it is necessary to note that microfluidic-sorted spermatozoa are in the process of capacitation, as mentioned in this paper [ 36 ]. According to our previous paper [ 7 ], when the cAMP-PKA-mediated signaling activity is enhanced to promote the capacitation-related events in bull spermatozoa, sperm motility temporally becomes slower and less linear (like the transitional sinuous type) at the early stage of the capacitation and finally becomes hyperactivated after the completion of capacitation.…”

Section: Discussionmentioning

confidence: 99%

Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation

Yamada

Sakase

Fukushima

et al. 2018

Journal of Reproduction and Development

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Progressive movement of spermatozoa has conventionally been regarded as a good indicator of motility. However, bull spermatozoa exhibit two types of progressive movement: progressive/planar movement without rotation and progressive/helical movement with rotation. The aim of this study was to reconsider the evaluation criteria of bull ejaculated sperm motility in the context of rotation. Here, we compared the movement patterns of ejaculated spermatozoa with relatively high and low protein kinase A (PKA)-mediated signaling activities, because sperm motility is positively regulated by PKA-mediated signaling activities. We prepared sperm samples with high and low PKA-mediated signaling activities by suspending spermatozoa in media containing either the stimulator (NaHCO3) or inhibitor (KH-7) of adenylyl cyclase 10, and we then investigated movement patterns and relative velocities using a microscopic high-speed camera and recording system. In the control medium without NaHCO3 and KH-7, most spermatozoa exhibited round/planar movement without rotation and asymmetrical bends in the principal pieces. NaHCO3 significantly promoted changes in movement patterns from round/planar movement to progressive/planar movement (without rotation) as well as symmetrization of flagellar bends and increased relative velocities. KH-7 significantly increased spermatozoa exhibiting progressive/helical movement (with rotation), decreased relative velocities, and symmetrized flagellar bends with a reduction in their size. These indicate that progressive/planar movement (without rotation) and fast movement characterize the movement patterns of bull ejaculated spermatozoa with high PKA-mediated signaling activities. A sign of reduced PKA-mediated signaling activity is not only slow movement but also helical movement (with rotation). Thus, it is beneficial to add a new parameter of “rotation” to the evaluation criteria of bull ejaculated sperm motility.

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“…The test involves a unique combination of protein A and anti-human immunoglobulin M antibody conjugated to colloidal gold particles. [136] b. Erectile dysfunction and male infertility A microfluidic sperm sorting system was developed for the ultrahigh-throughput selection and separation of motile, DNA-intact, and functionally competent sperm. The low-dose insemination of microfluidic sorted spermatozoa improved fertility, leading to successful production of live births.…”

Section: Resultsmentioning

confidence: 99%

Nanotechnology in reproductive medicine: Opportunities for clinical translation

Shandilya

Pathak

Lohiya

et al. 2020

Clin Exp Reprod Med

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In recent years, nanotechnology has revolutionized global healthcare and has been predicted to exert a remarkable effect on clinical medicine. In this context, the clinical use of nanomaterials for cancer diagnosis, fertility preservation, and the management of infertility and other pathologies linked to pubertal development, menopause, sexually transmitted infections, and HIV (human immunodeficiency virus) has substantial promise to fill the existing lacunae in reproductive healthcare. Of late, a number of clinical trials involving the use of nanoparticles for the early detection of reproductive tract infections and cancers, targeted drug delivery, and cellular therapeutics have been conducted. However, most of these trials of nanoengineering are still at a nascent stage, and better synergy between pharmaceutics, chemistry, and cutting-edge molecular sciences is needed for effective translation of these interventions from bench to bedside. To bridge the gap between translational outcome and product development, strategic partnerships with the insight and ability to anticipate challenges, as well as an in-depth understanding of the molecular pathways involved, are highly essential. Such amalgamations would overcome the regulatory gauntlet and technical hurdles, thereby facilitating the effective clinical translation of these nano-based tools and technologies. The present review comprehensively focuses on emerging applications of nanotechnology, which holds enormous promise for improved therapeutics and early diagnosis of various human reproductive tract diseases and conditions.

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Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility

Cited by 67 publications

References 68 publications

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation

Nanotechnology in reproductive medicine: Opportunities for clinical translation

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