Live births after vitrification of oocytes in a stimulated in vitro fertilization–embryo transfer program

139

Purpose The objective of this retrospective study was to evaluate the efficacy of vitrification and slow freezing for the cryopreservation of human cleavage stage embryos in terms of post-warming survival rate, post-warming embryo morphology and clinical outcomes. Methods The embryos of 305 patients at cleavage stages were cryopreserved either with vitrification (153 patients) or slow-freezing (152 patients) methods. After warming; the survival rate, post-warmed embryo morphology, clinical pregnancy and implantation rates were evaluated and compared between the two groups. Result(s) In the vitrification group versus slow freezing group, the survival rate (96.9% vs. 82.8%) and the postwarmed excellent morphology with all blastomeres intact (91.8% vs. 56.2%) were higher with an odds ratio of 6.607 (95% confidence interval; 4.184-10.434) and 8.769 (95% confidence interval; 6.460-11.904), respectively. In this group, the clinical pregnancy rate (40.5% vs. 21.4%) and the implantation rate (16.6% vs. 6.8%) were also higher with an odds ratio of 2.427 (95%confidence interval; 1.461-4.033) and 2.726 (95% confidence interval; 1.837-4.046), respectively. Conclusion(s) Vitrification in contrast to slow freezing is an efficient method for cryopreservation of human cleavage stage embryos. Vitrification provides a higher survival rate, minimal deleterious effects on post-warming embryo morphology and it can improve clinical outcomes.

Section: Introductionmentioning

confidence: 99%

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Valojerdi

Eftekhari‐Yazdi

Karimian

et al. 2009

139

“…Clinical outcome Since the first successful pregnancy from frozen human oocyte was reported 20 years ago [1], remarkable technological progress has been made in the area of cryopreservation of human oocytes. The recent increased interest in vitrification has resulted in improved efficiency and pregnancy outcomes [2][3][4][5][6][7][8][9][10][11][12][13]. Being able to protect and prolong the reproductive capacity by way of oocyte cryopreservation potentially opens the door for many thousands of women who are at a risk of losing ovarian function from radiation, chemotherapy and surgery.…”

Section: Conclusion(s)mentioning

confidence: 99%

“…The recent increased interest in vitrification has resulted in improved efficiency and pregnancy outcomes [2][3][4][5][6][7][8][9][10][11][12][13]. Being able to protect and prolong the reproductive capacity by way of oocyte cryopreservation potentially opens the door for many thousands of women who are at a risk of losing ovarian function from radiation, chemotherapy and surgery.Over the last decade, multiple clinics have reported IVF successes after transfer of embryos derived from frozen/ thawed oocytes [5,[14][15][16][17][18][19][20]. The authors have recently reported that oocytes from young fertile women retain their reproductive potential after 6 months of vitrification cryopreservation [20] and the pregnancy and implantation rates are comparable to that of frozen embryos.…”

mentioning

confidence: 99%

“…Over the last decade, multiple clinics have reported IVF successes after transfer of embryos derived from frozen/ thawed oocytes [5,[14][15][16][17][18][19][20]. The authors have recently reported that oocytes from young fertile women retain their reproductive potential after 6 months of vitrification cryopreservation [20] and the pregnancy and implantation rates are comparable to that of frozen embryos.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Successful live birth from vitrified oocytes after 5 years of cryopreservation

Kim

Hong

2010

Self Cite

Objective To report a birth of a healthy boy after long-term cryopreservation of oocytes by vitrification. Design Clinical application. Setting IVF Center. Patient A 17 year-old female with secondary pulmonary hypertension caused by transposition of great vessels visited our center in 2002, and she wished oocytes cryopreservation to avoid possible sterility after the following category X medication treatment. Intervention(s) Vitrified oocytes on Electron Microscope (EM) grids were warmed after 5 years of storage. Surviving MII oocytes were microinjected for fertilization and two embryos were transferred into a gestational carrier day 5 after microinjection. Main Outcome Measure(s) Survival, fertilization, cleavage, clinical pregnancy and delivery. Result(s) Eleven out of fourteen oocytes (78.6%) survived warming. Eight Metaphase II (MII) oocytes and 3 in vitro matured oocytes were microinjected; all 11 oocytes (100%) fertilized and 2 embryos were transferred on day 5. A healthy baby boy weighing 3,600 g was delivered at 38 weeks of gestation. Live-birth rates per warmed oocyte and per injected oocyte were 7.1% and 9.1% respectively. Conclusion(s)Cryopreservation after vitrification with EM grids maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth.Keywords Human oocyte cryopreservation . Vitrification . Electron microscope grid . Ethylene glycol . Clinical outcome Since the first successful pregnancy from frozen human oocyte was reported 20 years ago [1], remarkable technological progress has been made in the area of cryopreservation of human oocytes. The recent increased interest in vitrification has resulted in improved efficiency and pregnancy outcomes [2][3][4][5][6][7][8][9][10][11][12][13]. Being able to protect and prolong the reproductive capacity by way of oocyte cryopreservation potentially opens the door for many thousands of women who are at a risk of losing ovarian function from radiation, chemotherapy and surgery.Over the last decade, multiple clinics have reported IVF successes after transfer of embryos derived from frozen/ thawed oocytes [5,[14][15][16][17][18][19][20]. The authors have recently reported that oocytes from young fertile women retain their reproductive potential after 6 months of vitrification cryopreservation [20] and the pregnancy and implantation rates are comparable to that of frozen embryos. There is evidence that children born from frozen embryos have no significantly increased risk of congenital abnormalities [21][22][23], however, no such data are available concerning children born from vitrified oocytes after long-term storage.Here, we report the healthy outcome of a now 2 year old boy who was born after vitrification of oocytes that were stored for 5 years.Capsule Documentation of a pregnancy after long-term storage of vitrified oocytes.

“…To date, more than 1,000 successful live births resulting from cryo-preserved human oocytes have been reported [2][3][4][5][6][7][8][9][10][11][12]. Vitrification seems to be a successful and promising technique for oocyte cryopreservation, resulting in several hundred healthy live births recently [10,11,[13][14][15]. With the improved efficiency of oocyte vitrification, it has been suggested that establishing oocyte cryo-banking with the oocyte vitrification method would be beneficial to many women [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Yan

Suzuki

et al. 2011

Purpose To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming. Methods Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8-10 days, 90-92 days and 180-182 days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120 h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared. Result(s)The oocyte cryo-survival rate declined following cryo-storage duration for 180-182 days (90.4±7.9%) compared to that of the other two groups (97.4±3.0% and 98.0± 3.3%). The fertilization rate in the group of 180-182 days (66.6±22.0%) was also significantly reduced (P<0.05) compared with the groups of 8-10 days (92.2±10.8%) and 90-92 days (94.7±9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P<0.05) following long cryo-storage duration (72.1± 8.2%, 25.2±3.8% and 5.5±13.6%, respectively). Conclusion(s) The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen.