Live birth of twins derived from zona-free oocytes

Hu, Yunxia; Trolice, Mark P.

doi:10.1016/j.fertnstert.2016.01.023

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…The complete absence of ZP in oocytes is a rare event in IVF treatment; however, oocytes with damaged or partially absent zona due to laboratory manipulations are not uncommon [1,5,7,13]. Consequently, much of the published experiences of ZP-free patients are cases of ZP damage and loss during handling, rather than a complete absence due to ZP production failure.…”

Section: Discussionmentioning

confidence: 99%

“…In comparison to a previous report regarding successful live birth of complete zona-free patients due to ZP production issue [7], this case study reported a set of modified protocols in detail along with full time-lapse footage of blastocyst development. Due to the rare occurrence of such case, statistical analysis with a reasonable sample size to demonstrate which culture environment is best for these oocytes is deemed to be too difficult to acquire.…”

Section: Discussionmentioning

confidence: 99%

“…ZP-free oocytes may be present in the assisted reproductive laboratory as a result of either ruptured ZP during in vitro manipulation [5], purposefully removed ZP [6], or ZP production issues in a rare patient population [7]. In all of these patients, the oocyte is very fragile due to the lack of a protective surrounding shell and requires very delicate handling.…”

Section: Introductionmentioning

confidence: 99%

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Live birth in a complete zona-free patient: a case report

Watson

Korman

Liu

et al. 2021

J Assist Reprod Genet

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Objective To report a live birth from a patient with complete zona-free oocytes due to abnormal zona production and to reveal full time-lapse blastocyst development footage of its originating embryo. Methods A 34-year-old woman presented with a history of failed fertilization via standard in vitro fertilization insemination and a potential absence of zona pellucida. A total of 3 intracytoplasmic sperm injection cycles were undertaken with all oocytes collected being zona-free. Embryos created in the initial 2 cycles were cultured in the G1+/G2+ sequential media in a benchtop incubator. During the final successful cycle, the culture strategy was shifted to single step media (G-TL) in an Embryoscope+ incubator. ResultsThe first 2 attempts led to a biochemical pregnancy or no blastocyst available for transfer. In the third cycle, 13 out of 24 collected oocytes were subjected to injection, with 4 being normally fertilized. Two blastocysts were subsequently formed, in which one was cryopreserved and the other transferred. A live baby girl (1570g) was subsequently delivered at 34 weeks of gestation by cesarean section. Conclusion Live birth can be achieved for patients with zona production deficiency. Adjustment in ovarian stimulation and subsequent embryo culture strategies may have potentially contributed to the success of the 3rd cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Live birth in a complete zona-free patient: a case report

Watson

Korman

Liu

et al. 2021

J Assist Reprod Genet

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“…In our study, we used the human-cloned hyaluronidase enzyme, which is reported to be less traumatic to unprotected oocytes and oolemmas (de Vos et al, 2008), and a diagnostic ICSI to both confirm the diagnosis of complete ZFO and assess the feasibility of ICSI and embryo development using ZP-free oocytes. Different manipulation procedures for genetically ZP-free oocytes and successful ICSI fertilization using non-denuded and partially denuded oocytes have been reported previously (Paz et al, 2004;Vajta et al, 2010;Hu and Trolice, 2016).…”

Section: Discussionmentioning

confidence: 99%

Role of diagnostic intracytoplasmic sperm injection (ICSI) in the management of genetically determined zona pellucida-free oocytes during in vitro fertilization: a case report

Metwalley

Brasha

Esteves³

et al. 2020

Zygote

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Purpose: To report the utilization of diagnostic intracytoplasmic sperm injection (D-ICSI), an ICSI cycle performed in the natural cycle, to obtain information about embryo development potential after sperm injection into zona pellucida (ZP)-free oocytes. Materials and Methods: We report the case of a couple with primary unexplained infertility with a history of previous failed, in vitro fertilization intracytoplasmic sperm injection (IVF-ICSI) cycles characterized by the presence of ZP-free oocytes. Whole exome sequencing (WES) was carried out to analyse the possible genetic basis of oocyte abnormality. Results: Diagnostic ICSI provided information about the embryo development potential from ZP-free oocytes and allowed better planning of the subsequent ICSI cycle. WES revealed that the absence of ZP was likely to be due to a new (ZP1) mutation. The subsequent ICSI cycle resulted in the delivery of a healthy baby. Discussion: To the best of our knowledge, our report is the first to describe the use of D-ICSI to determine the feasibility of embryo development and implantation in a patient with ZP1 mutation, resulting in the subsequent delivery of a healthy baby. We used ‘diagnostic’ ICSI in the normal menstrual cycle to explore the feasibility of embryo development after sperm injection into ZP-free oocytes. Our results may expand the spectrum of diagnostic procedures associated with unexplained infertility.

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“…In a previous report, no zygotes or two-cell-stage embryos that lacked a ZP survived storage in liquid nitrogen 8 . It is, however, possible for embryos to develop normally without a ZP, and the live birth of twins from autologous ZF oocytes was reported in 2016 9 . On the contrary, for assisted reproduction technology or reproductive biotechnology, the ZP is essential for handling oocytes or embryos with a micromanipulator.…”

Section: Introductionmentioning

confidence: 99%

Agarose capsules as new tools for protecting denuded mouse oocytes/embryos during handling and freezing-thawing and supporting embryonic development in vivo

Nagatomo

Yao

Araki³

et al. 2017

Sci Rep

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Oocytes without a zona pellucida (ZP) often occur as a result of congenital or operational effects, but they are difficult to handle, and embryonic survival is low. Such zona-free (ZF) oocytes are therefore not used in clinics or laboratories. Furthermore, in the laboratory, removal of the ZP is often necessary for genetic manipulation by viral infection or twin production by blastomere separation, but adverse effects on development have been reported. It would therefore be extremely valuable if the embryo could be covered with a structure similar to that of the ZP. In this study, we sought to determine whether an agarose capsule could serve as a substitute for the ZP. Our results indicate that embryos derived from these agarose capsules were able to develop normally, and could be transplanted to obtain viable offspring, without having to remove the agarose capsule. Furthermore, before compaction, the agarose capsule embryos exhibited good freezing tolerance, and survival rate was extremely high compared to ZF embryos. Thus, agarose capsules represent a valuable tool for utilizing oocytes and embryos that lack a ZP, both in a clinical and livestock setting.

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Live birth of twins derived from zona-free oocytes

Abstract: Patients with the rare condition of 100% zona-free oocytes maintain the potential for pregnancy after careful micromanipulation of the oocytes. Caution is recommended on the number of embryos selected for transfer to reduce the risk of multiple gestation.

Cited by 9 publications

References 11 publications

Live birth in a complete zona-free patient: a case report

Live birth in a complete zona-free patient: a case report

Role of diagnostic intracytoplasmic sperm injection (ICSI) in the management of genetically determined zona pellucida-free oocytes during in vitro fertilization: a case report

Agarose capsules as new tools for protecting denuded mouse oocytes/embryos during handling and freezing-thawing and supporting embryonic development in vivo

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