Live birth after transfer of vitrified-warmed embryo derived from vitrified-warmed oocyte and frozen-thawed sperm following failed ICSI: Case report

Abstract

ABSTRACT

Successful approach for couples having their own genetic offspring using vitrified‐warmed oocytes injected by frozen‐thawed poor quality testicular sperm: A case report

“…In one case reported by Zhang et al in 2017, the patient did not achieve pregnancy following injection of fresh oocytes with very low counts, immotile ejaculated sperms. However, after injection of the vitrified‐warmed sibling oocytes, with frozen‐thawed donor sperms, the pregnancy and live birth were achieved 1 . Similar to this case, when the ejaculated sperms or TESE sample have poor quality or resulted in unsatisfactory clinical outcomes, the use of a third person's sperm is recommended in almost all IVF centers worldwide 13 …”

“…Todays, by replacing the oocyte slow freezing cryopreservation method with vitrification and adjustment of freezing conditions in the field of assisted reproductive technology (ART), the survival rate of oocytes and embryos has dramatically improved 1 . Oocyte cryopreservation offers several options, including fertility preservation in women undergoing cancer treatment, for example, chemotherapy or radiotherapy, in egg donation cycles, for women who electively delay childbearing, for a male partner who cannot be present or unable to ejaculate, or in azoospermia case whom testicular sperm extraction (TESE) procedure is failed at the day of ovum pick up.…”

Oncofertility: An Area of Reproductive Medicine Capable to Protect the Fertility of Cancer Patients

“…However, primary oocytes are subjected to in vitro maturation (IVM) process, allowing the cultivation of cumulus-oocyte complexes (COC) that will complement oocyte factors to elicit the response of cumulus cells to meiosis-stimulating elements such as endothelial growth factors (VEGF), follicle stimulating hormone (FSH) and cyclic adenosine Monophosphate (cAMP) modulators [42,43]. This methodology had been studied and applied by several teams around the world, resulting in successful studies on women oocyte cryopreservation [8,34,44,45].…”

“…A study performed by Schuffnera and collaborators in 2004 [5] verified two cases of successful gestation after the semen was cryopreserved, prior to the oncological treatment. Subsequently, Zhang and collaborators in 2017 [45] made two attempts in one study. In the first study, an intracytoplasmic sperm injection (ICSI) was proposed for a couple.…”

“…Cryopreservation of embryos holds numerous reproductive success rates and is widely disseminated throughout the world. The success rates vary according to the patient's age, oocyte characteristics, time of implantation of the thawed embryos; ovarian reserve and treatment success (number of oocytes obtained and fertilized) according to [9,40,42,45,82].…”