Live birth after rescue in vitro maturation–intracytoplasmic sperm injection in type 1 diabetes, polycystic ovary syndrome patient using clomiphene–antagonist protocol

Sankari, Samundi; Elanchezhian, M.; Selvamani, Divya; Nagarajan, M; Gopikrishnan, D.

doi:10.4103/jhrs.jhrs_65_17

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…In addition, no significant effects of clinical factors on the maturation rate within 24 h were observed. Taken together, immature oocytes collected from routine COH cycles still have a great potential to reach maturation by in vitro culture, regardless of the clinical characteristics, ovarian stimulation protocol, and oocyte diameter, confirming the necessity and prospect of the R-IVM [ 3 , 16 – 21 ].…”

Section: Discussionmentioning

confidence: 93%

Analysis of maturation dynamics and developmental competence of in vitro matured oocytes under time-lapse monitoring

Yang

Zhu

Wang

et al. 2021

Reprod Biol Endocrinol

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Background To improve the developmental competence of in vitro cultured oocytes, extensive literature focused on maturation rate improvement with different additives in culture medium, while studies investigating the maturation dynamics of oocytes during in vitro maturation (IVM) and the influencing factors on oocyte viability are scarce. Methods The study involved a retrospective observation by time-lapse monitoring of the IVM process of 157 donated GV oocytes from 59 infertile couples receiving ICSI in 2019, in Tongji Hospital, Wuhan, China. The GV oocytes derived from controlled ovarian hyperstimulation (COH) cycles underwent rescue IVM (R-IVM), and the maturation dynamics, including GVBD time (GV-MI), time from GVBD to maturation (MI-MII), maturation time (GV-MII), and MII arrest duration (MII-ICSI), were recorded by time-lapse monitoring. The matured oocytes were inseminated at different MII arrest points and subsequent embryo developments were assessed. The effects of baseline clinical characteristics, oocyte diameters, and maturation dynamics on the developmental competence of the oocytes were also analyzed. Results Totally, 157 GV oocytes were collected. GVBD happened in 111 oocytes, with a median GV-MI duration of 3.7 h. The median MI-MII duration was 15.6 h and the median GV-MII duration was 19.5 h. The maturation rate reached 56.7% at 24 h and 66.9% at 48 h, and the clinical factors, including patient age, FSH level, AMH level, ovarian stimulation protocol, and serum estradiol and progesterone levels on hCG trigger day, showed no effects on the 24-h maturation rate. The normal fertilization rate of oocytes resuming meiosis within 8 h and matured within 24 h was significantly higher than that of oocytes resuming meiosis after 8 h and matured after 24 h. Furthermore, among those oocytes matured within 24 h, the high-quality embryo formation rate of oocytes resuming meiosis within 4.5 h and matured within 19 h was significantly higher. All stated time was measured from the start point of IVM. Additionally, for oocytes from patients with serum progesterone levels less than 1 ng/ml on hCG trigger day, the high-quality embryo formation rate was significantly increased. Conclusion R-IVM technology could increase the available embryos for patients in routine COH cycles, but excessive culture beyond 24 h is not recommended. GV-MI duration of the oocyte, recorded by time-lapse system, and serum progesterone levels of patients on hCG trigger day can significantly affect the developmental potential of the IVM oocytes.

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Section: Discussionmentioning

confidence: 93%

Analysis of maturation dynamics and developmental competence of in vitro matured oocytes under time-lapse monitoring

Yang

Zhu

Wang

et al. 2021

Reprod Biol Endocrinol

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“…We observed that in reproductive aging, mRNA GC content was associated with longer half-life and increased translational output. Although the RNA-seq data used in our analysis was from IVF patients whose GV oocytes failed to mature following hCG administration and were then rescued with in vitro maturation (Reyes et al 2017; Virant-Klun et al 2016), rescue in vitro maturation can result in pregnancies and live births (Lee et al 2016; Elanchezhian et al 2018). In the future it will be highly valuable to assess human reproductive aging in oocytes from healthy individuals in order to identify molecular signatures of advanced maternal age.…”

Section: Resultsmentioning

confidence: 99%

Codon composition in human oocytes reveals age-associated defects in mRNA decay

Alvarez

Brachova

Christenson

2021

Preprint

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Oocytes from women of advanced reproductive age have lower developmental potential, yet the underlying mechanisms of this phenomena are incompletely understood. Oocyte maturation is dependent upon translational control of stored maternal mRNA that were synthesized during oocyte growth. We observed that GC content of mRNA was negatively associated with half-life in oocytes from reproductively young women (< 30 years), contrastingly directly with oocytes from reproductively aged women (≥ 40 years) where mRNA half-lives were positively associated with GC nucleotide content. Additionally, we observed that mRNA half-lives were negatively associated with protein abundance in young oocytes, while GC content was positively associated with protein abundance in aged oocytes. Examination of codon composition during the GV-to-MII transition revealed that codons that facilitate rapid translation promoted mRNA stability and are considered optimal, while codons that slow translation destabilized mRNA, and are considered non-optimal. GC-containing codons were more optimal in reproductive aging, and also correlated positively with protein abundance. This study indicates that reproductive aging coincides with the stabilization of a subset of mRNA that have the potential to be over-translated during oocyte maturation, this is likely to lead to observed decreases in oocyte quality in older women. Because oocyte mRNA decay is translationally linked, this suggests that maternal aging causes defects in translation, which results in reduced translational efficiency and the retention of maternal mRNA that are normally degraded in oocytes from young women. In the case of oocytes, defects in translation can alter the RNA decay pathways and result in incorrect maternal mRNA dosage, which may negatively impact embryonic development.

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“…The exploration of treatment strategies for infertility of the patients with PCOS associated with diabetes is extremely significant for these females. The combination of IVM and intracytoplasmic sperm injection (ICSI) provided an effective method to overcome the challenges of infertility treatment in patients with PCOS associated with diabetes [ 11 ]. Therefore, IVM may be a promising method to avoid high glucose caused damage to oocytes and to provide oocytes for ART of patients with diabetes.…”

Section: Introductionmentioning

confidence: 99%

Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes

Guo,

Wang,

Chen

et al. 2024

Nutr. Diabetes

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Background The number of patients with type 1 diabetes rises rapidly around the world in recent years. Maternal diabetes has a detrimental effect on reproductive outcomes due to decreased oocyte quality. However, the strategies to improve the oocyte quality and artificial reproductive technology (ART) efficiency of infertile females suffering from diabetes have not been fully studied. In this study, we aimed to examine the effects of nicotinamide mononucleotide (NMN) on oocyte maturation of mouse with type 1 diabetes mouse and explore the underlying mechanisms of NMN’s effect. Methods Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes. The successful establishment of the models was confirmed by the results of body weight test, fasting blood glucose test and haematoxylin and eosin (H&E) staining. The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined. Immunofluorescence staining (IF) was performed to examine the reactive oxygen species (ROS) level, spindle/chromosome structure, mitochondrial function, actin dynamics, DNA damage and histone modification of oocytes, which are potential factors affecting the oocyte quality. The quantitative reverse transcription PCR (RT-qPCR) was used to detect the mRNA levels of Sod1, Opa1, Mfn2, Drp1, Sirt1 and Sirt3 in oocytes. Results The NMN supplementation increased the oocyte maturation rate of the mice with diabetes. Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes. Conclusion NMN could improve the maturation rate and quality of oocytes in STZ-induced diabetic mice, which provides a significant clue for the treatment of infertility of the patients with diabetes.

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Live birth after rescue in vitro maturation–intracytoplasmic sperm injection in type 1 diabetes, polycystic ovary syndrome patient using clomiphene–antagonist protocol

Cited by 3 publications

References 18 publications

Analysis of maturation dynamics and developmental competence of in vitro matured oocytes under time-lapse monitoring

Analysis of maturation dynamics and developmental competence of in vitro matured oocytes under time-lapse monitoring

Codon composition in human oocytes reveals age-associated defects in mRNA decay

Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes

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