Live attenuated H5N1 vaccine with H9N2 internal genes protects chickens from infections by both Highly Pathogenic H5N1 and H9N2 Influenza Viruses

Nang, Nguyen Tai; Song, Byung Min; Kang, Yun Hee; Kim, Heui Man; Kim, Hyun Soo; Seo, Sang Heui

doi:10.1111/j.1750-2659.2012.00363.x

Cited by 10 publications

(5 citation statements)

References 49 publications

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“…This was evident in reduced mortality and virus shedding. Unvaccinated chickens challenged with H9N2 shed virus at 2.5 log 10 EID 50 /mL similar to other studies using H9N2 viruses [41]. H5N2 vaccinated chickens developed substantial HI and NI antibody titers directed against both viruses upon vaccination with a heterologous H5N2 vaccine, and this provides a level of protection that is generally sufficient to prevent H5N1 and H9N2 infections.…”

Section: Discussionsupporting

confidence: 78%

Generation of a reassortant avian influenza virus H5N2 vaccine strain capable of protecting chickens against infection with Egyptian H5N1 and H9N2 viruses

Kandeil

Moatasim

Gomaa

et al. 2016

Vaccine

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Background Avian influenza H5N1 viruses have been enzootic in Egyptian poultry since 2006. Avian influenza H9N2 viruses which have been circulating in Egyptian poultry since 2011, showed high replication rates in embryonated chicken eggs and mammalian cells. Methods To investigate which gene segment was responsible for increasing replication, we constructed reassortant influenza viruses using the low pathogenic H1N1 PR8 virus as backbone and included individual genes from A/chicken/Egypt/S4456B/2011(H9N2) virus. Then, we invested this finding to improve a PR8-derived H5N1 influenza vaccine strain by incorporation of the NA segment of H9N2 virus instead of the NA of H5N1. The growth properties of this virus and several other forms of reassortant H5 viruses were compared. Finally, we tested the efficacy of this reassortant vaccine strain in chickens. Results We observed an increase in replication for a reassortant virus expressing the neuraminidase gene (N2) of H9N2 virus relative to that of either parental viruses or reassortant PR8 viruses expressing other genes. Then, we generated an H5N2 vaccine strain based on the H5 from an Egyptian H5N1 virus and the N2 from an Egyptian H9N2 virus on a PR8 backbone. This strain had better replication rates than an H5N2 reassortant strain on an H9N2 backbone and an H5N1 reassortant on a PR8 backbone. This virus was then used to develop a killed, oil-emulsion vaccine and tested for efficacy against H5N1 and H9N2 viruses in chickens. Results showed that this vaccine was immunogenic and reduced mortality and shedding. Discussion Our findings suggest that an inactivated PR8-derived H5N2 influenza vaccine is efficacious in poultry against H5N1 and H9N2 viruses and the vaccine seed replicates at a high rate thus improving vaccine production.

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Section: Discussionsupporting

confidence: 78%

Generation of a reassortant avian influenza virus H5N2 vaccine strain capable of protecting chickens against infection with Egyptian H5N1 and H9N2 viruses

Kandeil

Moatasim

Gomaa

et al. 2016

Vaccine

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“…Live attenuated H5N1 vaccine for the protection against H5N1 and H9N2 for chickens have been developed and tested. The vaccine expressing hemagglutinin and neuraminidase was found to protect chickens eliciting both humoral and cell mediated immunity . The distribution of intrinsically disordered regions in H1N1 influenza virus using the PONDR program was previously studied .…”

Section: Discussionmentioning

confidence: 99%

“…The vaccine expressing hemagglutinin and neuraminidase was found to protect chickens eliciting both humoral and cell mediated immunity. 29 The distribution of intrinsically disordered regions in H1N1 influenza virus using the PONDR program was previously studied. 30 It is known that intrinsically disordered regions increase immunogenicity.…”

Section: Metirngtynrrkyqee Srlerqkiegvklesementioning

confidence: 99%

B cell epitopes in the intrinsically disordered regions of neuraminidase and hemagglutinin proteins of H5N1 and H9N2 avian influenza viruses for peptide‐based vaccine development

Ramamurthy

Sankar

Abraham

et al. 2019

J of Cellular Biochemistry

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Avian influenza viruses (AIV) are very active in several parts of the globe and are the cause of huge economic loss for the poultry industry and also human fatalities. Three dimensional modeling was carried out for neuraminidase (NA) and hemagglutinin (HA) proteins of AIV. The C‐score, estimated TM‐Score, and estimated root‐mean‐square deviation (RMSD) score for NA of H5N1 were −1.18, 0.57 ± 0.15, and 9.8 ± 7.6, respectively. The C‐score, estimated TM‐Score, and estimated RMSD score for NA of H9N2 were −1.43, 0.54 ± 0.15, and 10.5 ± 4.6, respectively. The C‐score, estimated TM‐Score, and estimated RMSD score for HA of H5N1 were −0.03, 0.71 ± 0.12, and 7.7 ± 4.3, respectively. The C‐score, estimated TM‐Score, and estimated RMSD score for HA of H9N2 were −0.57, 0.64 ± 0.13, and 8.9 ± 4.6, respectively. Intrinsically disordered regions were identified for the NA and HA proteins of H5N1 and H9N2 with the use of PONDR program. Linear B cell epitope was predicted using BepiPred 2 program for NA and HA of H5N1 and H9N2 avian influenza strains. Discontinuous epitopes were predicted by Discotope 2 program. The linear epitopes that were considered likely to be immunogenic and within the intrinsically disordered region for the NA of H5N1 was TKSTNSRSGFEMIWDPNGWTGTDSSFSVK, and for H9N2 it was VGDTPRNDDSSSSSNCRDPNNERGAP. In the case of HA of H5N1, it was QRLVPKIATRSKVNGQSG and ATGLRNSPQRERRRKK; for H9N2 it was INRTFKPLIGPRPLVNGLQG and SLKLAVGLRNVPARSSR. The discontinuous epitopes of NA of H5N1 and H9N2 were identified at various regions of the protein structure spanning from amino acid residue positions 90 to 449 and 107 to 469, respectively. Similarly, the discontinuous epitopes of HA of H5N1 and H9N2 were identified in the amino acid residue positions 27 to 517 and 136 to 521, respectively. This study has identified potential and highly immunogenic linear and conformational B‐cell epitopes towards developing a vaccine against AIV both for human and poultry use.

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“…A variety of approaches have been used to develop vaccines for chickens and ducks, including reverse genetic expression of H5 and N1, DNA vectors expressing H5, infectious laryngotracheitis virus expressing H5, nonpathogenic H5-subtype influenza virus, plantexpressed H5, Herpes virus of turkeys expressing H5, fowlpox virus expressing H5, Newcastle disease virus expressing H5, and baculovirus virus-like particles expressing H5 (6,8,13,25,27,31,42).…”

Section: Introductionmentioning

confidence: 99%

Protective Efficacy of a Single Dose of Baculovirus Hemagglutinin-Based Vaccine in Chickens and Ducks Against Homologous and Heterologous H5N1 Virus Infections

et al. 2014

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Outbreaks of the highly pathogenic H5N1 virus in poultry and humans are ongoing. Vaccination is an efficient method for prevention of H5N1 infection. Using chickens and ducks, we assessed the efficacy of a vaccine comprising H5N1 hemagglutinin (HA) protein produced in a baculovirus expression system. The immunized chickens and ducks were protected against lethal infection by H5N1 in an antigen dose-dependent manner. Complete protection against homologous challenge and partial protection against heterologous challenge were achieved in chickens immunized with 5 μg HA protein and in ducks immunized with 10 μg HA protein. The IgG antibody subtype was mainly detected in the sera and tissues, including the lungs. The neuraminidase (NA) inhibition assay was negative in immunized chickens and ducks. Our results indicated that the expressed HA protein by baculovirus was immunogenic to both chickens and ducks, and the immunized chickens and ducks were protected from the lethal infections of highly pathogenic H5N1 influenza virus, though ducks required more HA protein than chickens to be protected. Also, baculovirus HA-vaccinated poultry can be differentiated from infected poultry by NA inhibition assay.

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Live attenuated H5N1 vaccine with H9N2 internal genes protects chickens from infections by both Highly Pathogenic H5N1 and H9N2 Influenza Viruses

Cited by 10 publications

References 49 publications

Generation of a reassortant avian influenza virus H5N2 vaccine strain capable of protecting chickens against infection with Egyptian H5N1 and H9N2 viruses

Generation of a reassortant avian influenza virus H5N2 vaccine strain capable of protecting chickens against infection with Egyptian H5N1 and H9N2 viruses

B cell epitopes in the intrinsically disordered regions of neuraminidase and hemagglutinin proteins of H5N1 and H9N2 avian influenza viruses for peptide‐based vaccine development

Protective Efficacy of a Single Dose of Baculovirus Hemagglutinin-Based Vaccine in Chickens and Ducks Against Homologous and Heterologous H5N1 Virus Infections

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