Litter Commensal Bacteria Can Limit the Horizontal Gene Transfer of Antimicrobial Resistance to Salmonella in Chickens

Oladeinde, Adelumola; Abdo, Zaid; Zwirzitz, Benjamin; Woyda, Reed; Lakin, Steven M.; Press, Maximilian O.; Cox, N. A.; Thomas, Jesse C.; Looft, Torey; Rothrock, Michael J.; Zock, Gregory; Lawrence, Jodie Plumblee; Cudnik, Denice; Ritz, Casey W.; Aggrey, Samuel E.; Grove, Jonas R; Wiersma, Crystal J

doi:10.1128/aem.02517-21

Cited by 13 publications

(10 citation statements)

References 58 publications

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“…Fluoroquinolones resistant was mediated by efflux pumps that reduce the permeability of the antibiotic (Yedekci et al, 2012). The results of the current study confirmed that a high percentage of MDR and XDR K. pneumoniae was detected which may due to the ability of bacterial cells to acquisition of resistance genes that transmitted horizontally or vertically where the horizontal transfer of genes is the most effective and dangerous, as it works on spreading genes easily between different strains, either through plasmids or transposons (Oladeinde et al, 2022).…”

Section: Resultssupporting

confidence: 73%

The Effect of Ceftriaxon and Gentamicin on Antibiotic Resistance Pattern of Multiple Drug Resistance Klebsiella pneumoniae

audi,

Naeem

2023

Egyptian Academic Journal of Biological Sciences. C, Physiology

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Klebsiella pneumoniae is one of the bacterial species with high antibiotic resistance. The present study aimed to investigate the effect of Minimal Inhibitory Concentration (MIC) of Ceftriaxon and Gentamicin on the antibiotic pattern of multi-drug resistant K. pneumoniae isolated from urinary tract infections. Sixty K. pneumoniae isolates have been isolated from 300 urine specimens collected from patients with urinary tract infections. Vitek 2 Compact System was used for identification and detection of antibiotic resistance patterns of bacterial isoltes. The highest percentage of antibiotic resistance of bacterial isolates to ampicillin (100%), followed by cefazolin (53.3%), while the lowest percentage of resistance was shown to Imipenem and tigecycline, which were 5% and 3.3%, respectively. The effect of MIC of gentamicin and ceftriaxon toward 10 selected isolates were used to select mutated isolates, then the effect of both antibiotic-on-antibiotic resistance of mutated isolates was detected using Vitek 2 Compact System. The results of the mutation experiment using ceftriaxone gave successful results to 7 isolates while 5 isolates gave positive results to gentamicin. The results of antibiotic resistance pattern of mutated isolates showed that all mutated isolates became resistance to most tested antibiotics in comparison with original isolates. We concluded that indiscriminate and wrong use of antibiotics would lead to emergence of MDR isolates not only for antibiotics belonging to the same class but also to other classes.

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Section: Resultssupporting

confidence: 73%

The Effect of Ceftriaxon and Gentamicin on Antibiotic Resistance Pattern of Multiple Drug Resistance Klebsiella pneumoniae

audi,

Naeem

2023

Egyptian Academic Journal of Biological Sciences. C, Physiology

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“…Jurburg et al (2019) also reported that Escherichia/Shigella and Streptococcus were the major taxa found in fecal samples of one-day old broiler chicks. Similarly; Cárdenas-Rey et al (2022), showed that Escherichia/Shigella was in high abundance in the ceca of one-day old broiler chicks (relative abundance of 37.3% ± 24.0%). Together, these results suggest that the bacterial taxa found in the meconium of day-one old chicks in the study were under selection in the hatchery.…”

Section: Discussionmentioning

confidence: 81%

Temporal dynamics of the cecal and litter microbiome of chickens raised in two separate broiler houses

Zwirzitz

Oladeinde²,

Johnson³

et al. 2023

Front. Physiol.

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In this study, we investigated the dynamics of the ceca and litter microbiome of chickens from post-hatch through pre-harvest. To achieve this, six hundred one-day old Cobb 500 broiler chicks were raised on floor pens for 49 days in two separate houses. We performed short-read and full-length sequencing of the bacterial 16S rRNA gene present in the meconium and in cecal and litter samples collected over the duration of the study. In addition, we determined the antimicrobial resistance (AMR) phenotype of Escherichia coli and Enterococcus spp. isolated from the meconium and the ceca of 49-day old chickens. We monitored the relative humidity, temperature, and ammonia in each house daily and the pH and moisture of litter samples weekly. The overall microbial community structure of the ceca and litter consistently changed throughout the course of the grow-out and correlated with some of the environmental parameters measured (p < 0.05). We found that the ceca and litter microbiome were similar in the two houses at the beginning of the experiment, but over time, the microbial community separated and differed between the houses. When we compared the environmental parameters in the two houses, we found no significant differences in the first half of the growth cycle (day 0–21), but morning temperature, morning humidity, and ammonia significantly differed (p < 0.05) between the two houses from day 22–49. Lastly, the prevalence of AMR in cecal E. coli isolates differed from meconium isolates (p < 0.001), while the AMR phenotype of cecal Enterococcus isolates differed between houses (p < 0.05).

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“…Early studies on the competitive exclusion properties of reused litter have shown a lower survival of Salmonella in reused litter compared to fresh litter ( Tucker, 1967 ; Fanelli et al, 1970 ; Gustafson and Kobland, 1984 ; Soerjadi-Liem and Cumming, 1984 ; Corrier et al, 1992 ). Recent studies have shown that chickens raised on reused litter harbor a different microbiome than chicks grown on fresh litter ( Cressman et al, 2010 ; Wang et al, 2016 ; Oladeinde et al, 2022 ) and that the number of litter samples positive for Salmonella significantly decreases as the number of litter reuses increased compared with the first use of the litter ( Roll et al, 2011 ; Chinivasagam et al, 2012 ; Muniz et al, 2014 ; Oladeinde et al, 2022 ). Roberts et al (2013) detected Salmonella at a greater frequency with the first flock of broilers raised on fresh pine shavings compared to flock 2 and 3.…”

Section: Discussionmentioning

confidence: 99%

Management and environmental factors influence the prevalence and abundance of food-borne pathogens and commensal bacteria in peanut hull-based broiler litter

Oladeinde

Awosile²,

Woyda³

et al. 2023

Poultry Science

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Litter Commensal Bacteria Can Limit the Horizontal Gene Transfer of Antimicrobial Resistance to Salmonella in Chickens

Cited by 13 publications

References 58 publications

The Effect of Ceftriaxon and Gentamicin on Antibiotic Resistance Pattern of Multiple Drug Resistance Klebsiella pneumoniae

The Effect of Ceftriaxon and Gentamicin on Antibiotic Resistance Pattern of Multiple Drug Resistance Klebsiella pneumoniae

Temporal dynamics of the cecal and litter microbiome of chickens raised in two separate broiler houses

Management and environmental factors influence the prevalence and abundance of food-borne pathogens and commensal bacteria in peanut hull-based broiler litter

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