Lithocarpus�polystachyus Rehd. leaves aqueous extract protects against hydrogen peroxide‑induced SH-SY5Y cells injury through activation of Sirt3 signaling pathway

Abstract

Lithocarpus polystachyus Rehd. (sweet tea; ST) leaves is a type of chinese folkloric medicine from southern china. The purpose of the present study was to explore the neuroprotective effect of ST, and to explore its underlying mechanisms in hydrogen peroxide (H 2 O 2)-induced neuronal cell injury in cultured human neuroblastoma. H 2 O 2 was used as oxidant inducer and human SH-SY5Y neuroblastoma cells were treated with various concentrations of ST. cell viability and cell death were detected using MTT and LdH assays, respectively. Additionally, the production of intracellular and mitochondrial reactive oxygen species (ROS) were determined by 2' ,7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and MitoSOX Red, respectively. The production of malondialdehyde (MdA), reduced glutathione (GSH) level, glutathione peroxidase (GSH-Px), superoxide dismutase (SOd) activities, and NAd + /NADH ratio were confirmed using relevant kits. The expression of adenosine monophosphate-activated protein kinase (AMPK), peroxisome proliferator-activated receptor coactivator (PGc)-1α, Sirt3, isocitrate dehydrogenase (IdH)2, forkhead boxO3a (Foxo3a), and SOd2 were analyzed by western blot analysis. It was demonstrated that pre-treatment with ST enhanced cell viability and repressed cell death, and it also reduced intracellular and mitochondrial ROS accumulation. Additionally, ST attenuated MdA production and enhanced GSH level, GSH-Px and SOd activities. Furthermore, ST not only increased NAd + /NAdH ratio, but also inhibited the decrease of AMPK, PGc-1α, Sirt3, IdH2, Foxo3a, and SOd2. The present study revealed that ST exerts protective effects against oxidative stress-induced SH-SY5Y cells injury, and the underlying mechanisms are, at least partly, associated with its antioxidant capacity and function through mitochondrial Sirt3 signaling pathway.

The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies

“…In 2017, sweet tea was listed as a new food material by the National Health and Family Planning Commission of China. It contains plentiful polysaccharides, flavonoids, and polyphenolic ingredients, which have extensive biological properties, such as anti-diabetic, anti-hypertensive, neuroprotective, hepatoprotective, and anti-aging effects [3][4][5]. As some of the main bioactive components in sweet tea, sweet tea polysaccharides (STPs), however, have not been intensively investigated in respect of their structural and biological properties.…”

Effects of Microwave-Assisted Extraction Conditions on Antioxidant Capacity of Sweet Tea (Lithocarpus polystachyus Rehd.)

“…These compounds are the main bioactive components of sweet tea and also major contributors to its sweetness [ 3 ]. Sweet tea and its bioactive components have been found to have antioxidant, antimicrobial, anti-inflammatory, antidiabetic, anticancer and cardiovascular protective activities [ 4 , 5 , 6 , 7 , 8 , 9 ]. The effective extraction of bioactive components in sweet tea is helpful for its exploitation and utilization.…”

Fermented Mentha arvensis administration provides neuroprotection against transient global cerebral ischemia in gerbils and SH-SY5Y cells via downregulation of the MAPK signaling pathway

“…The human neuroblastoma (SH-SY5Y) cell death model mediated by H 2 O 2 is widely used to study neuronal cell death caused by oxidative stress and neuroprotective effects of natural products [ 36 , 37 ]. The present study results indicated the H 2 O 2 -induced SH-SY5Y cell death was significantly reduced by pretreatment with FMA.…”