Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo

Abstract

Lithocarpus polystachyus leaves have been used as tea beverage and folk medicine for healthy care in the Southwest of China. The purpose of this study is to investigate the anticancer activity of Lithocarpus polystachyus Rehd leaf aqueous extract (LPAE) and to explore the possible mechanism of its activity. Growth inhibition effects of LPAE breast cancer were tested in vitro and in vivo. The possible mechanism of its activity was analyzed with cell biological and molecular biological assays. After LPAE treatment, the proliferation and colony formation of cancer cells decreased; apoptotic cells increased; DNA fragmentations were evident; mRNA and protein expressions of PPARγ, Bax, and caspase-3 genes increased and expressions of cyclin D1 and Bcl-2 genes decreased; in vivo experiment, LPAE inhibited human beast cancer growth. The findings in this experimental study suggested that LPAE has potential cytotoxic and apoptotic effects on human breast cancer cells in vitro and inhibits the cancer growth in vivo, and its mechanism of activity might be associated with apoptosis induction of cancer cells through upregulation of the mRNA and protein expressions of PPARγ, Bax, and capase-3 genes and downregulation of the expressions of cyclin D1 and Bcl-2 genes.

Bioactive Triterpenoids from the Leaves and Twigs of Lithocarpus litseifolius and L. corneus

“…The crude extract was partitioned between water and ethyl acetate, and the ethyl acetate soluble fraction was separated by repeated column chromatography. As a result, four new triterpenoids (1)(2)(3)(4), three triterpenoids [3,4-seco-4(23),20(29)-lupadiene-3,28dioic acid (5), 3,4-seco-olean-4(23),12-diene-3,28-dioic acid (6), and 3,4-seco-ursan-4(23),12-diene-3,28-dioic acid (7)] were isolated for the first time from a natural source [12,13] (▶ Fig. 1), and six known compounds [3-epi-betulinic acid (8), betulinic acid (9), betulonic acid (10), cylicodiscic acid (11), lupeol (12), and 3epi-glutinol (13)] were identified [14][15][16][17][18][19].…”

“…The obvious differences between them were the 1 H and 13 C NMR shifts of C-3 in 3β,20-dihydroxylupane-28-oic acid (δ H 3.55, t, J = 9.0 and δ C 78.3) slightly changed in 2 (δ H 3.61, t, J = 4.0 and δ C 75.3), which suggested that 2 is a stereoisomer of 3β,20-dihydroxylupane-28-oic acid. In the COSY spectrum, the three proton sequences H 2 -1 (δ H 1 Fig. 3…”

“…The genus Lithocarpus (Fagaceae) distributed in East Asia comprises more than 300 species, among which 15 species are found in Taiwan. In previous pharmacological studies, Lithocarpus plants were reported to have anti-tumor [1], anti-hyperglycemic [2], anti-hypertensive [3], anti-inflammatory [4], and anti-oxidant [5] activities. Triterpenes [6][7][8][9], steroids [7], chalcones [10], and various phenolic compounds [4] were found to be major constituents of Lithocarpus plants.…”

Analisis Fitokimia Dan Toksisitas Dari Ekstrak Daun Tumbuhan Mempening (Lithocarpus bancanus (Scheff.) Rehd) Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

“…Menurut studi literatur terhadap spesies yang berbeda dari tumbuhan ini memiliki berbagai macam aktivitas. Daun dari tumbuhan Lithocarpus polystachus Rehd memiliki aktivitas antidiabetes (Dong et al, 2012), aktivitas antioksidan (Sun et al, 2015) dan antikanker (Lin et al, 2014), Lithocarpus celebicus memiliki aktivitas antimikroba (Khan et al, 2001).…”

Antioxidant and Antidiabetic Activities of Mempening (Lithocarpus bancanus) Leaves

“…Likewise, bioactivities from the genus have been evaluated including antioxidants, antidiabetic, anticancer, antimicrobial and other activities. [5][6][7][8] In regard to explore antidiabetic agent from Talang Mamak medicinal plants 9 , we reported the antioxidant and antidiabetic activity of L. bancanus leaves extract and fractions.…”