Lithium stimulation of diffusion chamber colony growth is mediated by factors other than colony‐stimulating factor

Doukas, Michael; Shadduck, Richard K.; Waheed, Abdül; Gass, Cecelia

doi:10.1002/stem.5530070304

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…In facts, lithium increases CSF production by peripheral blood and bone marrow human mononuclear cells acting via phagocytes [20]. In the murine diffusion chamber granulopoiesis model, a mechanism not involving GM-CSF was suggested [21,22]. A lithium concentration of 4 mM gave the greatest enhancing effect on colony formation in CSFstimulated cultures, and a concentration Ͼ1 mM inhibited de novo synthesis of CSF by monocytes [23].…”

Section: Quantitative Effectsmentioning

confidence: 99%

Lithium and hematology: established and proposed uses

Focosi

Azzarà

Kast

et al. 2008

Journal of Leukocyte Biology

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Lithium (as lithium carbonate) is an inexpensive drug, widely used in psychiatry for over 50 years in treatment of mood instability (bipolar disorder) and as an adjunct to antidepressants. Hematological effects of neutrophilia and increased circulating CD34+ cells of marrow origin have long been known. Lithium was at the center of hematological investigations in the 1980s, but no definitive use in hematology has yet emerged. We review evidence that lithium increases G-CSF and augments G-CSF effects. We suggest possible therapeutic uses of lithium in neutropenia. In bone marrow transplantation, preharvest lithium-assisted hematopoietic stem cell mobilization may be useful as well.

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Section: Quantitative Effectsmentioning

confidence: 99%

Lithium and hematology: established and proposed uses

Focosi

Azzarà

Kast

et al. 2008

Journal of Leukocyte Biology

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Diffusion chamber colony‐forming unit (cfu‐d): A primitive stem cell

Niskanen¹,

Chatelain²,

Symann³

1989

The International Journal of Cell Cloning

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Assay of hematopoietic precursor cells in diffusion chambers (DCs) implanted intraperitoneally in experimental animals provides a powerful tool for studying stem cell kinetics in vivo. In this system, the effect of cell migration (which complicates whole animal studies) is eliminated because the membranes utilized in the construction of the chambers are impermeable for cells, while permitting free passage of molecules present in the humoral phase of the host. As judged by light microscopy, conditions in the DC cultures primarily favor macrophage and granulocyte growth. However, the use of in vitro and in vivo subculture to further analyze chamber contents has demonstrated that the system supports proliferation of early hematopoietic progenitors. Additionally, cells capable of rescuing lethally irradiated mice proliferate in DC cultures. Development of the plasma clot DC technique has revealed that most of the growth occurs in colonies which are derived from single cells (CFU-d). Characterization of these cells indicates that they are at least as primitive as other colony-forming cells and, also based on subculture studies, can differentiate along several hematopoietic lineages. In addition to normal CFU-d, both embryonal and leukemic cells can give rise to granulocytes, macrophages, megakaryocytes and erythroid cells in the DC cultures. Evaluation of the effects of humoral factors on hematopoietic cell proliferation and differentiation in the system has led to the identification of both stimulators and inhibitors that may be different from the well-characterized cytokines. Thus, the system seems to be useful for detecting molecules controlling the most primitive stages of hematopoiesis. We believe that the DC culture technique holds enormous potential in the study of stem cell proliferation and differentiation in vivo.

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The immune system is a key factor in the etiology of psychosocial disease

Smith

1991

Medical Hypotheses

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Lithium stimulation of diffusion chamber colony growth is mediated by factors other than colony‐stimulating factor

Cited by 6 publications

References 13 publications

Lithium and hematology: established and proposed uses

Lithium and hematology: established and proposed uses

Diffusion chamber colony‐forming unit (cfu‐d): A primitive stem cell

The immune system is a key factor in the etiology of psychosocial disease

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