Listeriosis

Lorber, Bennett

doi:10.1093/clinids/24.1.1

Cited by 442 publications

(283 citation statements)

References 87 publications

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“…For intracellular growth assays, incubation of WISH cells in gentamicin-containing medium was prolonged for an additional period of 4,6,8,24,48, or 72 h at 37°C followed by lysis and CFU counts. Intracellular growth was expressed as replication index (RI) corresponding to the number of CFU at a given time post infection divided by the number of CFU at time 0 (1 h post infection).…”

Section: Intracellular Growthmentioning

confidence: 99%

Invasive Pathway ofListeria Ivanoviiin Human Amnion-Derived Wish Cells

Ammendolia¹,

Superti²,

Bertuccini³

et al. 2007

Int J Immunopathol Pharmacol

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Among Listeria genus, only two species, Listeria ivanovii and Listeria monocytogenes, are pathogenic. L. ivanovii is almost only associated with infections in animals, mainly sheep and cattle, and has rarely been associated with human infections, whereas L. monocytogenes causes severe illnesses in both humans and animals. To further investigate the pathogenetic features of L. ivanovii in humans, we undertook a study in which the intracellular behaviour of this pathogen was analysed in WISH cells, a cell line derived from human amniotic tissue, and compared to that of L. monocytogenes. Using microbiological, biochemical, and ultrastructural approaches, we demonstrate that L. ivanovii can adhere to and invade human amniotic cells, lyse the phagosomal membrane, polymerize host cell actin, and spread from cell to cell more efficiently than L. monocytogenes. However, although L. ivanovii is capable of specifically infecting and replicating in human amnion cells, its survival in cytoplasm is limited compared to that of L. monocytogenes.

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Section: Intracellular Growthmentioning

confidence: 99%

Invasive Pathway ofListeria Ivanoviiin Human Amnion-Derived Wish Cells

Ammendolia¹,

Superti²,

Bertuccini³

et al. 2007

Int J Immunopathol Pharmacol

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“…It grows and survives in extreme conditions, such as a wide range of temperatures from 21?5 to 50 uC, a pH range of 4?3-9?6 (Donnelly, 2001) and high salt concentrations, and can tolerate freezing and drying, making it an ideal candidate for transmission through food and rendering it a difficult organism to eliminate from food chains. Transmission from mother to fetus is the only documented form of person-to-person transmission (Lorber, 1997). Food-borne listeriosis has become a great concern to public health and food industries due to a number of outbreaks and sporadic cases caused by foods contaminated with L. monocytogenes (Lorber, 1997;Schlech, 2000;Donnelly, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Transmission from mother to fetus is the only documented form of person-to-person transmission (Lorber, 1997). Food-borne listeriosis has become a great concern to public health and food industries due to a number of outbreaks and sporadic cases caused by foods contaminated with L. monocytogenes (Lorber, 1997;Schlech, 2000;Donnelly, 2001). Although listeriosis is a rare human illness, it is a leading cause of mortality from a food-borne pathogen (Mead et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…However, none of the mAbs reported to date are specific for L. monocytogenes strains with the exception of mAb EM-7G1 described by Bhunia & Johnson (1992). Almost all human infections are attributed to serotypes 4b, 1/2a and 1/2b (Lorber, 1997;Donnelly, 2001), with serotype 4b strains accounting for approximately 40 % of cases (Rocourt & Bille, 1997). Thus, immunoassays specific for serotype 4b strains are very useful for epidemiological investigations.…”

Section: Introductionmentioning

confidence: 99%

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Monoclonal antibodies binding to the cell surface of Listeria monocytogenes serotype 4b

Lin¹,

Todoric²,

Mallory³

et al. 2006

Journal of Medical Microbiology

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Serotype 4b strains of the food-borne pathogen Listeria monocytogenes are responsible for a large portion of sporadic listeric infections and all major food-borne listeriosis outbreaks in humans. Hybridomas were produced from three fusions with lymphocytes of ND4 mice immunized either with the insoluble antigens of L. monocytogenes serotype 4b or with formalin-killed bacterial cells and screened for monoclonal antibodies (mAbs) reactive to L. monocytogenes serotype 4b. A set of 35 mAbs was identified by ELISA as having reactivity with both the insoluble antigen fraction and the whole-cell antigens. Thirteen of these mAbs belonged to immunoglobulin subclass G1 (IgG1), fifteen were IgG2a and seven mAbs were IgM. Only 20 out of the 35 mAbs were capable of detecting protein bands of various sizes ranging from 20 to 88 kDa in Western blots. Two of these mAbs, M2365 and M2367, were capable of binding to cell-surface antigens of live L. monocytogenes serotype 4b, as demonstrated by immunofluorescence microscopy and immunogold transmission electron microscopy. Immunofluorescence microscopy showed that M2365 and M2367 failed to bind to the cell surfaces of Escherichia coli O157 : H7, Salmonella enterica (serotype Typhimurium DT104) or Campylobacter jejuni. Evaluation of the cross-reactions of all 35 mAbs with whole-cell antigens of E. coli O157 : H7, S. Typhimurium, C. jejuni and Listeria innocua by ELISA indicated that the majority of the mAbs, including M2365 and M2367, did not cross-react with E. coli O157 : H7, S. Typhimurium or C. jejuni and showed no or a very weak reaction with L. innocua. Furthermore, M2365 and M2367 showed no reaction with whole-cell antigens derived from L. monocytogenes serotypes 1/2a, 1/2b and 3a, and from Listeria grayi, Listeria ivanovii and Listeria seeligeri, in an ELISA. Collectively, these data suggest that M2365 and M2367 have potential use in the development of immunological methods of laboratory diagnosis for L. monocytogenes serotype 4b in clinical or food samples.

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“…Listeria monocytogenes est la bactérie responsable de la listériose humaine, une infection d'origine alimentaire touchant préférentiellement les sujets aux ages extrêmes de la vie, les femmes enceintes, et les patients immunodéprimés [1]. La gravité de la listériose humaine tient à la capacité qu'a cette bactérie intracellulaire facultative d'atteindre le système nerveux central et l'unité foeto-placentaire.…”

unclassified

Un modèle transgénique pour la listériose humaine : rôle de l’interaction entre l’internaline et la E-cadhérine dans la traversée de la barrière intestinale par L. monocytogenes

Lecuit

Cossart

2001

Med Sci (Paris)

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Listeriosis

Cited by 442 publications

References 87 publications

Invasive Pathway ofListeria Ivanoviiin Human Amnion-Derived Wish Cells

Invasive Pathway ofListeria Ivanoviiin Human Amnion-Derived Wish Cells

Monoclonal antibodies binding to the cell surface of Listeria monocytogenes serotype 4b

Un modèle transgénique pour la listériose humaine : rôle de l’interaction entre l’internaline et la E-cadhérine dans la traversée de la barrière intestinale par L. monocytogenes

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