Listeria monocytogenes in milk and dairy products

Kasalica, Anka; Vukovic, V.; Vranješ, Anka Popović; Memiši, N.

doi:10.2298/bah1103067k

Cited by 41 publications

(31 citation statements)

References 42 publications

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“…The inter- and intra-farm movement of animals and/or farmers is an important contribution in the listeria dispersion throughout the farm [33]. In addition to the feces, [40, 42, 43], animal drinking water [42, 44, 45], feeds or feed components [40, 45], sawdust bedding, farmyard manure, soil in which fodder plants were grown, cattle feed such as silage hay cubes, beet pulp and wheat lees [46], pest and wildlife could disseminate L. monocytogenes in their feces [33, 47, 48] are possible sources and routes of L. monocytogenes infection for the animals. The success in the control of the previous sources of animal, farm or/and environmental contamination with L. monocytogenes would thereby be in the welfare of the farm animals and farm environment.…”

Section: Discussionmentioning

confidence: 99%

Determination of virulence and antibiotic resistance pattern of biofilm producing Listeria species isolated from retail raw milk

et al. 2016

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BackgroundOne of the foodborne pathogens is Listeria monocytogenes, which causes serious invasive illness in elderly and immunocompromised patients, pregnant women, newborns and infants ranking second after salmonellosis because of its high case fatality rate. Listerial cow mastitis marked by abnormal milk, increased cell counts and reduced production has not been reported. Therefore, apparently healthy cows can be reservoirs of L. monocytogenes. A number of 203 udder milk samples from apparently healthy animals (buffalo, n = 100; cow, n = 103) were collected and tested for Listeria. Isolated colonies on the PALCAM agar were Listeria species confirmed according to their biochemical and the Christie–Atkins–Munch-Petersen (CAMP) reactions. The Listeria species pathogenicity of was tested by phosphatidylinositol-specific phospholipase C, DL-alanine-β-naphthylamide HCl, Dalanine-p-nitroanilide tests, chick embryo, mice inoculation tests, Vero cell cytotoxicity and biofilm formation. The virulence-associated genes, hlyA, plcB, actA and iap associated with Listeria were molecularly assayed.ResultsThe 17 isolated Listeria spp. represented a prevalence rate of 8.4 %. Of these 3 (1.4 %), 2 (1 %), 5 (2.5 %), 4 (2 %) and 3 (1.5 %) were confirmed as L. monocytogenes, L. innocua, L. welshimeri, L. seelegeri, respectively. While the L. monocytogenes isolate displayed all the four virulence-associated genes, L. seelegeri carried the hlyA gene only. The L. monocytogenes had a strong in vitro affinity to form a biofilm, in particular serotype 4 which is associated with human infections. L. monocytogenes showed resistance for 9/27 antibiotics.ConclusionsThe biofilm forming capability of the Listeria spps. makes them particularly successful in colonizing surfaces within the host thus being responsible for persistence infections and due to their antimicrobial resistant phenotype that this structure confers. In addition, strains belonging to serotypes associated with human infections and characterized by pathogenic potential (serotype 4) are capable to persist within the processing plants forming biofilm and thus being a medical hazard.

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Section: Discussionmentioning

confidence: 99%

Determination of virulence and antibiotic resistance pattern of biofilm producing Listeria species isolated from retail raw milk

et al. 2016

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“…It is widespread in the environment and has been isolated from water, soil, dust, plants, animal feed, feces and sewage and has been associated with mammals, birds and possibly fish [1]. This pathogen has also been isolated from food products like unpasteurized (raw) milk or foods made from unpasteurized milk, red meats, poultry, seafood, vegetables, fruits and ready-to-eat food products [2][3][4]. Of these, milk and other dairy products have been implicated in about half of all the listeriosis outbreaks and in several sporadic cases worldwide [5,6].…”

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confidence: 99%

Prevalence and Antimicrobial Susceptibility of Listeria spp. in Dairy Food Products and Water Samples in Dhaka, Bangladesh

Sarker¹,

Ahmed²

2015

JLS

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A total of 57 samples, of which 17 were surface water samples and 40 were dairy food samples (raw milk, pasteurized milk, icecream, sweet, milk based drink like matha and borhani) were tested for the isolation of Listeria spp. Putative Listeria isolates were identified by conventional microbiological tests and Analytical Profile Index. Overall prevalence of Listeria spp. in both food and water samples were 8.77%, of which one was (1.75%) Listeria monocytogenes, 2 (3.5%) were Listeria innocua and 2 were (3.5%) Listeria welshimeri. When compared between two types of samples, water samples contained two Listeria spp. (11.76%) of which one was pathogenic Listeria monocytogenes and the other was Listeria innocua. In case of food samples, three Listeria spp. (7.5%) were isolated of which one was Listeria innocua (icecream sample) and two were Listeria welshimeri (icecream sample and raw milk). No Listeria was found in pasteurized milk, sweet, matha and borhani. Antibiotic resistance profile of the Listeria isolates showed that 60% isolates were resistant to Ampicillin and Erythromycin, 20% isolates were Sulphamethoxazole and Ciprofloxacin resistant. No resistance was observed to Chloramphenicol for any Listeria isolates.

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“…Contamination of milk by these bacteria can also be of faecal origin. Paralysis and circling movement was observed in sheep affected by listeriosis in late stage of the disease [41].…”

Section: In Animalsmentioning

confidence: 98%

Growing Importance of Listeriosis as Food borne Disease

Ayele¹,

Kundu²,

Jadhav³

2017

J Exp Food Chem

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Foodborne diseases are among the most widespread public health problems and are human infections transmitted through ingested food, and caused by pathogens whose natural reservoir is a vertebrate animal species. Globally, foodborne diseases resulted in over 600 million illnesses and 420,000 deaths in 2010.The majority of the pathogens causing microbial foodborne diseases are considered to be zoonotic. Presently, there are more than 350 zoonoses of multiple etiologies, which are reported from developing as well as developed nations of the world. However, listeriosis has received special attention due to the severity of infections resulting into high morbidity and mortality. Among the various species of the genus Listeria, Listeria monocytogens is recognized as the principal cause of listeriosis in humans as well as animals including birds. The most common route of infection of humans is consumption of foods contaminated by L. monocytogenes. Raw milk, pasteurized milk, soft cheeses, ice cream, meat and meat products, fish and fish products, eggs and egg products, fruits and vegetables may serve as important sources of L. monocytogenes. The organism can survive and grow over a wide range of environmental conditions such as refrigeration temperatures, low pH and high salt concentration. This allows the pathogen to overcome food preservation and safety barriers, and pose a potential risk to human health. In humans, it can lead to meningitis, encephalitis, septicemia, abortion, pneumonia, endocarditis, urethritis, diarrhea, conjunctivitis, and skin infections. The vulnerable groups are pregnant women, infants, older persons, and individuals with weakened immune system. It is recommended to undertake more sincere efforts to make sure the safe handling of various foods to minimize the contamination of L. monocytogenese, which has emerged as an important foodborne pathogen of global significance.

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Listeria monocytogenes in milk and dairy products

Cited by 41 publications

References 42 publications

Determination of virulence and antibiotic resistance pattern of biofilm producing Listeria species isolated from retail raw milk

Determination of virulence and antibiotic resistance pattern of biofilm producing Listeria species isolated from retail raw milk

Prevalence and Antimicrobial Susceptibility of Listeria spp. in Dairy Food Products and Water Samples in Dhaka, Bangladesh

Growing Importance of Listeriosis as Food borne Disease

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