Listeria ivanovii ATCC 19119 strain behaviour is modulated by iron and acid stress

Longhi, Catia; Ammendolia, Maria Grazia; Conte, Maria Pia; Seganti, L; Iosi, Francesca; Superti, Fabiana

doi:10.1016/j.fm.2014.02.015

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…Currently, we are identifying the specific Hfq-binding sRNAs that are involved in pathways for intercellular stress survival and pathogenesis. Our observations are also consistent with previous findings indicating that bacterial virulence is not only influenced by dedicated virulence factors, but is also dependent on protein expression suited to specific environmental stresses [6,46,47].…”

Section: Discussionsupporting

confidence: 93%

hfq regulates acid tolerance and virulence by responding to acid stress in Shigella flexneri

Yang

Wang

et al. 2015

Research in Microbiology

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Shigella flexneri is an important etiological agent of bacillary dysentery in developing countries. The Hfq protein is thought to play a major regulatory role in various cellular processes in this organism. However, the roles of Hfq in stress tolerance and virulence in S. flexneri in response to environmental stress have not been fully studied. In this study, hfq was highly expressed when S. flexneri was exposed to low pH. Growth retardation was observed in the hfq deletion mutant at pH values ranging from 5.0 to 7.0 and the survival rate of the mutant strain was reduced by 60% in acidic conditions (pH 3.0) compared with the wild-type strain. Additionally, competitive invasion assays in HeLa cells and lung invasion assays showed that the virulence of the hfq deletion mutant was significantly decreased. An evaluation of the mechanism revealed that, along with the expression of the Type III secretion system genes, acid resistance genes were also increased with acid stress. Interestingly, a statistically strong linear correlation was observed between the expression of hfq and Type III secretion system genes, as well as between hfq and acid resistance genes, under various pH conditions. In this study, we provide evidence that Hfq regulates genes related to acid resistance for survival under acid stress and controls virulence through the positive regulation of Type III secretion systems. Importantly, we propose that hfq is a key factor in maximal adaptation to host acid stress during infection, regulating acid stress tolerance and virulence in response to acid stress in S. flexneri.

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Section: Discussionsupporting

confidence: 93%

hfq regulates acid tolerance and virulence by responding to acid stress in Shigella flexneri

Yang

Wang

et al. 2015

Research in Microbiology

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“…To date, studies examining the virulence characteristics of L. ivanovii have examined individual reference strains rather than collections of isolates. These studies indicate that L. ivanovii is capable of cellular invasion, often at levels in excess of L. monocytogenes [ 29 – 31 ]. L. ivanovii is also capable of lysis of the host cell phagosome and actin polymerization but is perhaps less effective than L. monocytogenes in cell-to-cell spread and intracellular multiplication [ 29 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…These studies indicate that L. ivanovii is capable of cellular invasion, often at levels in excess of L. monocytogenes [ 29 – 31 ]. L. ivanovii is also capable of lysis of the host cell phagosome and actin polymerization but is perhaps less effective than L. monocytogenes in cell-to-cell spread and intracellular multiplication [ 29 , 31 , 32 ]. The findings of this study support previous studies and demonstrated that some wild-type isolates of L. ivanovii are more invasive than a clinical L. monocytogenes reference isolate (EGDe).…”

Section: Discussionmentioning

confidence: 99%

Occurrence, Persistence, and Virulence Potential ofListeria ivanoviiin Foods and Food Processing Environments in the Republic of Ireland

Álvarez‐Ordóñez

Leong

Morgan

et al. 2015

BioMed Research International

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The aim of this study was to assess the occurrence of L. ivanovii in foods and food processing environments in Ireland, to track persistence, and to characterize the disease causing potential of the isolated strains. A total of 2,006 samples (432 food samples and 1,574 environmental swabs) were collected between March 2013 and March 2014 from 48 food business operators (FBOs) belonging to different production sectors (dairy, fish, meat, and fresh-cut vegetable). Six of the forty-eight FBOs had samples positive for L. ivanovii on at least one sampling occasion. L. ivanovii was present in fifteen samples (fourteen environmental samples and one food sample). All but one of those positive samples derived from the dairy sector, where L. ivanovii prevalence was 1.7%. Six distinguishable pulsotypes were obtained by PFGE analysis, with one pulsotype being persistent in the environment of a dairy food business. Sequence analysis of the sigB gene showed that fourteen isolates belonged to L. ivanovii subsp. londoniensis, while only one isolate was L. ivanovii subsp. ivanovii. Cell invasion assays demonstrated that the majority of L. ivanovii strains were comparable to L. monocytogenes EGDe in their ability to invade CACO-2 epithelial cells whilst four isolates had significantly higher invasion efficiencies.

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“…Acid-adapted cells showed a higher percentage of internalization in CaCo-2 cells when iron was added to the culture medium. Iron depletion enhanced the capacity of the bacterium to invade amniotic cells, regardless of acid adaptation or not [36].…”

Section: Ivanovii Persistence and Tolerance To Harsh Conditionsmentioning

confidence: 94%

Virulence Characteristics and Distribution of the Pathogen Listeria ivanovii in the Environment and in Food

et al. 2022

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Listeria ivanovii and L. monocytogenes, are the only pathogenic species of the genus Listeria and share many virulence factors and mechanisms of pathogenicity. L. ivanovii shows host tropism towards small ruminants and rodents and much lower virulence for humans compared to L. monocytogenes. However, severe infections caused by L. ivanovii, resulting in bacteremia, abortion and stillbirth, occasionally occurred in immunocompromised persons and in pregnant women, while in immunocompetent hosts L. ivanovii can cause gastroenteritis. In this review, the updated knowledge on virulence aspects and distribution of L. ivanovii in the environment and in food is summarized. Recent research on its virulence characters at genome level gave indications on how pathogenicity evolved in this bacterial species. As for L. monocytogenes, L. ivanovii infections occurred after the ingestion of contaminated food, so an overview of reports regarding its distribution in food products was carried out to obtain indications on the categories of foods exposed to contamination by L. ivanovii. It was found that a wide variety of food products can be a source of this microorganism and that, like L. monocytogenes, L. ivanovii is able to persist in the food production environment. Studies on its ability to grow in enrichment and isolation media suggested that its occurrence in nature might be underestimated. Moreover, virulence varies among strains for differences in virulence character regulation, presence/absence of genetic regions and the possible instability of a Listeria pathogenicity genomic island, LIPI-2, which is unique to L. ivanovii. We can conclude that L. ivanovii, as a possible pathogen for animals and humans, requires more focused investigations regarding its occurrence in the environment and in food and on intra-species variability of pathogenic potential.

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Listeria ivanovii ATCC 19119 strain behaviour is modulated by iron and acid stress

Cited by 6 publications

References 32 publications

hfq regulates acid tolerance and virulence by responding to acid stress in Shigella flexneri

hfq regulates acid tolerance and virulence by responding to acid stress in Shigella flexneri

Occurrence, Persistence, and Virulence Potential ofListeria ivanoviiin Foods and Food Processing Environments in the Republic of Ireland

Virulence Characteristics and Distribution of the Pathogen Listeria ivanovii in the Environment and in Food

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