Listeria ilorinensis sp. nov., isolated from cow milk cheese in Nigeria

Raufu, Ibrahim A.; Moura, Alexandra; Valès, Guillaume; Ahmed, Olayiwola Akeem; Aremu, Abdulfatai; Thouvénot, Pierre; Tessaud-Rita, Nathalie; Bracq-Dieye, Hélène; Krishnamurthy, Ramar; Leclercq, Alexandre; Lecuit, Marc

doi:10.1099/ijsem.0.005437

Cited by 18 publications

(14 citation statements)

References 38 publications

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“…1 The animal-species origin of milk was not specified. 2 The 244 samples of BTM originated from only four different farms.…”

Section: Isolation Frequency and Populations Of L Monocytogenes In Bo...mentioning

confidence: 99%

Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece

Angelidis,

Grammenou,

Kotzamanidis

et al. 2023

Pathogens

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The prevalence of Listeria monocytogenes in bovine bulk-tank milk (BTM) in Greece has not been previously investigated. The aim of the study was to estimate the prevalence of L. monocytogenes in bovine BTM in Greece and to characterize the isolates in terms of carriage of genes encoding for pathogenic determinants, assess the isolates’ biofilm-forming ability and determine their susceptibility against 12 antimicrobials. Samples (n = 138) of bovine BTM were obtained from farms located throughout Northern Greece and were analyzed qualitatively and quantitatively for L. monocytogenes. Five samples (3.6%) tested positive for L. monocytogenes. The pathogen’s populations in these positive samples were below 5 CFU/mL. Most isolates belonged to the molecular serogroup “1/2a, 3a”. All isolates carried the virulence genes inlA, inlC, inlJ, iap, plcA and hlyA, but actA was detected in only three isolates. The isolates displayed weak to moderate biofilm-forming ability and distinct antimicrobial resistance profiles. All isolates were characterized as multidrug resistant, with resistance to penicillin and clindamycin being a common feature. Considering that L. monocytogenes constitutes a serious public health threat, the key findings of the study, related to the carriage of virulence genes and multidrug resistance, highlight the importance of continued monitoring of the pathogen in farm animals.

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“…1 The animal-species origin of milk was not specified. 2 The 244 samples of BTM originated from only four different farms.…”

Section: Isolation Frequency and Populations Of L Monocytogenes In Bo...mentioning

confidence: 99%

Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece

Angelidis,

Grammenou,

Kotzamanidis

et al. 2023

Pathogens

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“…Listeriosis is a systemic infection caused by the Gram-positive bacterium Listeria monocytogenes . The genus Listeria comprises at least 26 species [1–4], and is ubiquitous in plant, water and soil environments [5]. L.…”

Section: Introductionmentioning

confidence: 99%

“…Listeriosis is a systemic infection caused by the Gram-positive bacterium Listeria monocytogenes. The genus Listeria comprises at least 26 species [1][2][3][4], and is ubiquitous in plant, water and soil environments [5]. L. monocytogenes and Listeria ivanovii are the two species within this genus known to cause infection in humans and animals, with L. monocytogenes being the leading cause of human clinical listeriosis [6].…”

Section: Introductionmentioning

confidence: 99%

Genomic epidemiology of the clinically dominant clonal complex 1 in the Listeria monocytogenes population in the UK

Fotopoulou,

Jenkins,

Barker

et al. 2024

Microbial Genomics

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Listeria monocytogenes is a food-borne pathogen, typically affecting the elderly, immunocompromised patients and pregnant women. The aim of this study was to determine the population structure of L. monocytogenes clonal complex 1 (CC1) in the UK and describe the genomic epidemiology of this clinically significant CC. We interrogated a working dataset of 4073 sequences of L. monocytogenes isolated between January 2015 and December 2020 from human clinical specimens, food and/or food-production environments. A minimum spanning tree was reconstructed to determine the population structure of L. monocytogenes in the UK. Subsequent analysis focused on L. monocytogenes CC1, as the cause of the highest proportion of invasive listeriosis in humans. Sequencing data was integrated with metadata on food and environmental isolates, and information from patient questionnaires, including age, sex and clinical outcomes. All isolates either belonged to lineage I (n=1299/4073, 32%) or lineage II (n=2774/4073, 68%), with clinical isolates from human cases more likely to belong to lineage I (n=546/928, 59%) and food isolates more likely to belong to lineage II (n=2352/3067, 77%). Of the four largest CCs, CC1 (n=237) had the highest proportion of isolates from human cases of disease (CC1 n=160/237, 67.5 %; CC121 n=13/843, 2 %; CC9 n=53/360, 15 %; CC2 n=69/339, 20%). Within CC1, most cases were female (n=95/160, 59%, P=0.01771) and the highest proportion of cases were in people >60 years old (39/95, 41%, P=1.314×10−6) with a high number of them aged 20–39 years old (n=35/95, 37%) most linked to pregnancy-related listeriosis (n=29/35, 83%). Most of the male cases were in men aged over 60 years old (40/65, 62%), and most of the fatal cases in both males and females were identified in this age group (42/55, 76%). Phylogenetic analysis revealed 23 5 SNP single linkage clusters comprising 80/237 (34 %) isolates with cluster sizes ranging from 2 to 19. Five 5 SNP clusters comprised isolates from human cases and an implicated food item. Expanding the analysis to 25 SNP single linkage clusters resolved an additional two clusters linking human cases to a potential food vehicle. Analysis of demographic and clinical outcome data identified CC1 as a clinically significant cause of invasive listeriosis in the elderly population and in women of child-bearing age. Phylogenetic analysis revealed the population structure of CC1 in the UK comprised small, sparsely populated genomic clusters. Only clusters containing isolates from an implicated food vehicle, or food processing or farming environments, were resolved, emphasizing the need for clinical, food and animal-health agencies to share sequencing data in real time, and the importance of a One Health approach to public-health surveillance of listeriosis.

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“…Recently, new species of Listeria spp. have been isolated, such as L. valentina (Quereda et al, 2020), L. ilorinensis (Raufu et al, 2022) and L. cossartiae, L. farberi, L. immobilis, L. portnoyi and L. rustica (Carlin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, identification and antimicrobial resistance of Listeria monocytogenes and Listeria spp. isolated from poultry and pork meat

Martinez‐Laorden,

Arraiz‐Fernandez,

Cantalejo

et al. 2024

Int J of Food Sci Tech

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SummaryThe aim of this work was to evaluate the prevalence of Listeria monocytogenes and other Listeria spp. in chicken, duck, quail, turkey and pork meat, including the antibiotic resistance of isolated strains. A total of 184 meat samples were collected from different retailers in La Rioja (Spain). The presence of Listeria spp. and L. monocytogenes were detected in 24.46% and 10.32% of the meat samples respectively. L. monocytogenes was the predominant Listeria spp. found in chicken, quail and pork meat, while L. innocua and L. welshimeri were the predominant species in duck and turkey meat respectively. A total of thirty‐three strains (55.93%) of Listeria spp. were found to be multi‐resistant (resistant to ≥3 families of antibiotics). The highest multi‐resistant rates were observed in L. monocytogenes (73.68%) and L. innocua (70.59%), followed by L. ivanovii (50%). Resistance to ampicillin and trimethoprim–sulfamethoxazole were found in L. monocytogenes strains isolated from chicken, being of special concern, since these antibiotics are used in the treatment of listeriosis. Special measures should be taken to reduce meat contamination such as adequate handling, correct preparation (cooking) and cleaning and disinfection in order to avoid cross‐contamination.

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Listeria ilorinensis sp. nov., isolated from cow milk cheese in Nigeria

Cited by 18 publications

References 38 publications

Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece

Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece

Genomic epidemiology of the clinically dominant clonal complex 1 in the Listeria monocytogenes population in the UK

Prevalence, identification and antimicrobial resistance of Listeria monocytogenes and Listeria spp. isolated from poultry and pork meat

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