Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs

Dix, Carly I; Soundararajan, Harish Chandra; Dzhindzhev, Nikola S.; Begum, Farida; Suter, Beat; Ohkura, Hiroyuki; Stephens, Elaine; Bullock, Simon L.

doi:10.1083/jcb.201211052

Cited by 78 publications

(119 citation statements)

References 88 publications

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“…7C,D). Qualitatively, the defects in apical h mRNA transport in dop mutants are similar to those exhibited by embryos in which components of the dynein-based mRNA transport machinery are compromised, including the RNAbinding protein Egalitarian, the adaptor protein Bicaudal D and the dynein co-factor Lissencephaly-1 (Bullock et al, 2006;Dix et al, 2013). These data provide direct evidence that Dop is important for normal dynein-dependent transport in the early embryo.…”

Section: Dop Is Required For Dynein-based Mrna Transport In the Embryosupporting

confidence: 61%

The Drosophila MAST kinase Drop out is required to initiate membrane compartmentalisation during cellularisation and regulates dynein-based transport

Hain¹,

Langlands²,

Sonnenberg³

et al. 2014

Journal of Cell Science

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Cellularisation of the Drosophila syncytial blastoderm embryo into the polarised blastoderm epithelium provides an excellent model with which to determine how cortical plasma membrane asymmetry is generated during development. Many components of the molecular machinery driving cellularisation have been identified, but cell signalling events acting at the onset of membrane asymmetry are poorly understood. Here we show that mutations in drop out (dop) disturb the segregation of membrane cortical compartments and the clustering of E-cadherin into basal adherens junctions in early cellularisation. dop is required for normal furrow formation and controls the tight localisation of furrow canal proteins and the formation of F-actin foci at the incipient furrows. We show that dop encodes the single Drosophila homologue of microtubule-associated Ser/Thr (MAST) kinases. dop interacts genetically with components of the dynein/dynactin complex and promotes dynein-dependent transport in the embryo. Loss of dop function reduces phosphorylation of Dynein intermediate chain, suggesting that dop is involved in regulating cytoplasmic dynein activity through direct or indirect mechanisms. These data suggest that Dop impinges upon the initiation of furrow formation through developmental regulation of cytoplasmic dynein.

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Section: Dop Is Required For Dynein-based Mrna Transport In the Embryosupporting

confidence: 61%

The Drosophila MAST kinase Drop out is required to initiate membrane compartmentalisation during cellularisation and regulates dynein-based transport

Hain¹,

Langlands²,

Sonnenberg³

et al. 2014

Journal of Cell Science

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“…In addition, mutations in Dop and Wech alter net transport of droplets, and quantitative tracking of individual droplets should reveal if this is the result of changed run length. Finally, the dynein cofactor Lis1 was recently shown to play an important role in run-length control for dynein in RNA transport during embryonic stages corresponding to IIa and IIb [82]; it is not yet clear if Lis1 might also influence run length for droplet motion: overall distribution of droplets was normal, suggestive of little effect, but quantitation of the motion at the single droplet level has yet to be reported. Thus, even without a comprehensive list of proteins involved in droplet motion, there are already many potential leads available to probe the molecular nature of the switch.…”

Section: Discussionmentioning

confidence: 99%

“…2B). Microtubule minus ends are associated with the MTOCs close to the plasma membrane, and plus ends point into the embryo interior, resulting in a radial array of microtubules with largely uniform polarity [57, 82]. By cycle 14, nuclei are densely packed at the surface, and microtubules are arranged grossly parallel around the whole embryo periphery.…”

Section: Introductionmentioning

confidence: 99%

As the fat flies: The dynamic lipid droplets of Drosophila embryos

Welte

2015

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Research into lipid droplets is rapidly expanding, and new cellular and organismal roles for these lipid storage organelles are continually being discovered. The early Drosophila embryo is particularly well suited for addressing certain questions in lipid-droplet biology and combines technical advantages with unique biological phenomena. This review summarizes key features of this experimental system and the techniques available to study it, in order to make it accessible to researchers outside this field. It then describes the two topics most heavily studied in this system, lipid-droplet motility and protein sequestration on droplets, discusses what is known about the molecular players involved, points to open questions, and compares the results from Drosophila embryo studies to what it is known about lipid droplets in other systems.

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“…In neurons, however, Lis1 is required for transport all along the axon, not just in regions of increased microtubule dynamicity (Moughamian et al, 2013; Pandey and Smith, 2011). Lis1 likely acts directly on dynein, priming the motor for transport (Huang et al, 2012) and/or recruiting the dynein/dynactin complex onto certain cargos (Dix et al, 2013). In sensory neurons, the spectraplakin BPAG1n4 and the endosomal protein retrolinkin have also been reported to be required for sustained retrograde transport along the axon, in a mechanism that also depends on dynamic microtubule plus ends (Kapur et al, 2014).…”

Section: Regional Specificity Of Axonal Transportmentioning

confidence: 99%

Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation

Maday

Twelvetrees

Moughamian

et al. 2014

Neuron

580

576

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Axonal transport is essential for neuronal function, and many neurodevelopmental and neurodegenerative diseases result from mutations in the axonal transport machinery. Anterograde transport supplies distal axons with newly synthesized proteins and lipids, including synaptic components required to maintain presynaptic activity. Retrograde transport is required to maintain homeostasis by removing aging proteins and organelles from the distal axon for degradation and recycling of components. Retrograde axonal transport also plays a major role in neurotrophic and injury response signaling. This review provides an overview of the axonal transport pathway and discusses its role in neuronal function.

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Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs

Abstract: Lissencephaly-1 promotes the interaction of dynein with dynactin and facilitates motor complex association with mRNA cargos.

Cited by 78 publications

References 88 publications

The Drosophila MAST kinase Drop out is required to initiate membrane compartmentalisation during cellularisation and regulates dynein-based transport

The Drosophila MAST kinase Drop out is required to initiate membrane compartmentalisation during cellularisation and regulates dynein-based transport

As the fat flies: The dynamic lipid droplets of Drosophila embryos

Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation

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