Lissajous Scanning Two-photon Endomicroscope for In vivo Tissue Imaging

Kim, Daniel Youngsuk; Hwang, Kyungmin; Ahn, JoongHo; Seo, Yeong‐Hyeon; Kim, Jae‐Beom; Lee, So‐Young; Yoon, Jin-Hui; Kong, Eunji; Jeong, Yong Hoon; Jon, Sangyong; Kim, Pilhan; Jeong, Ki‐Hun

doi:10.1038/s41598-019-38762-w

Cited by 43 publications

(35 citation statements)

References 37 publications

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“…As an example of this technology, a clinical-grade commercial endomicroscopic device has recently been commercialized (NvisionVLE ® Imaging System, Merit Medical Systems, Inc., South Jordan, UT, USA) [46]. Finally, (3) a two-photon endomicroscope has been recently validated and allows in-depth tissue imaging [47].…”

Section: New Precision Imaging Modalities For Ards: In Vivo Endomimentioning

confidence: 99%

In Vivo Endomicroscopy of Lung Injury and Repair in ARDS: Potential Added Value to Current Imaging

Lesur

Chagnon

Lebel

et al. 2019

JCM

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Background: Standard clinical imaging of the acute respiratory distress syndrome (ARDS) lung lacks resolution and offers limited possibilities in the exploration of the structure–function relationship, and therefore cannot provide an early and clear discrimination of patients with unexpected diagnosis and unrepair profile. The current gold standard is open lung biopsy (OLB). However, despite being able to reveal precise information about the tissue collected, OLB cannot provide real-time information on treatment response and is accompanied with a complication risk rate up to 25%, making longitudinal monitoring a dangerous endeavor. Intravital probe-based confocal laser endomicroscopy (pCLE) is a developing and innovative high-resolution imaging technology. pCLE offers the possibility to leverage multiple and specific imaging probes to enable multiplex screening of several proteases and pathogenic microorganisms, simultaneously and longitudinally, in the lung. This bedside method will ultimately enable physicians to rapidly, noninvasively, and accurately diagnose degrading lung and/or fibrosis without the need of OLBs. Objectives and Methods: To extend the information provided by standard imaging of the ARDS lung with a bedside, high-resolution, miniaturized pCLE through the detailed molecular imaging of a carefully selected region-of-interest (ROI). To validate and quantify real-time imaging to validate pCLE against OLB. Results: Developments in lung pCLE using fluorescent affinity- or activity-based probes at both preclinical and clinical (first-in-man) stages are ongoing—the results are promising, revealing correlations with OLBs in problematic ARDS. Conclusion: It can be envisaged that safe, high-resolution, noninvasive pCLE with activatable fluorescence probes will provide a “virtual optical biopsy” and will provide decisive information in selected ARDS patients at the bedside.

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Section: New Precision Imaging Modalities For Ards: In Vivo Endomimentioning

confidence: 99%

In Vivo Endomicroscopy of Lung Injury and Repair in ARDS: Potential Added Value to Current Imaging

Lesur

Chagnon

Lebel

et al. 2019

JCM

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“…>1 MHz). Thus, using resonant Lissajous scanning patterns 27 , we foresee the possibility for volumetric imaging at kHz rates.…”

Section: Discussionmentioning

confidence: 99%

Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

et al. 2020

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In optical microscopy, the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging. Recently, by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens (ETL) to the back focal plane, rapid axial scanning has been achieved. However, mechanical actuation of a mirror limits the axial scanning rate (usually only 10–100 Hz for piezoelectric or voice coil-based actuators), while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging. In an effort to overcome these limitations, we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging. Using a galvanometric mirror, we scan a laser beam laterally in a remote-focusing arm, which is then back-reflected from different heights of a mirror in the image space. We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude, allowing the quantification of rapid vesicular dynamics in three dimensions. We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope, which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution.

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“…Our first practical demonstration on microscopic imaging was ASLM, which has been criticized for its slow acquisition speed (around 10Hz framerate) 17 . Here, we demonstrate that our new scan technology allows one order of magnitude acceleration while keeping the high spatial resolving power of this emerging imaging technology.…”

Section: Discussionmentioning

confidence: 99%

Converting lateral scanning into axial focusing to speed up 3D microscopy

Chakraborty

Chang

Daetwyler

et al. 2020

Preprint

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In optical microscopy, the slow axial scanning rate of the objective or the sample has traditionally limited the speed of 3D volumetric imaging. Recently, by conjugating either a movable-mirror to the image plane or an electrotuneable lens (ETL) to the back-focal plane respectively, rapid axial scanning has been achieved. However, mechanical actuation of a mirror limits axial scanning rate (usually only 10-100 Hz for piezoelectric or voice coil based actuators), while ETLs introduce spherical and higher order aberrations, thereby preventing high-resolution imaging. Here, we introduce a novel optical design that can transform a lateral-scan motion into a spherical-aberration-free, high-resolution, rapid axial scan. Using a galvanometric mirror, we scan a laser beam laterally in a remote-focusing arm, which is then back-reflected from different heights of a mirror in image space. We characterize the optical performance of this remote focusing technique and use it to accelerate axially swept light-sheet microscopy (ASLM) by one order of magnitude, allowing the quantification of rapid vesicular dynamics in 3D.

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Lissajous Scanning Two-photon Endomicroscope for In vivo Tissue Imaging

Cited by 43 publications

References 37 publications

In Vivo Endomicroscopy of Lung Injury and Repair in ARDS: Potential Added Value to Current Imaging

In Vivo Endomicroscopy of Lung Injury and Repair in ARDS: Potential Added Value to Current Imaging

Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

Converting lateral scanning into axial focusing to speed up 3D microscopy

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