Liraglutide Immobilized on Poly(lactic-co-glycolic acid) Polymer Films Induced the Differentiation of Islet β-Like Cells from Bone Marrow Mesenchymal Stem Cells

Sun, Shuang; Cui, Weiwei; Dong, Ying; Wang, Qing

doi:10.1007/s13233-019-7061-0

Cited by 3 publications

(2 citation statements)

References 37 publications

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“… 54 Similar control or amelioration of organoid development using PEG has been achieved for intestinal organoids, 59 , 60 lung organoids, 61 mammary epithelial cell acini, 62 and renal tubules, 63 albeit producing smaller‐sized organoids. 58 , 60 Polycaprolactone, alone 64 or blended with chitosan, 65 can also support organoid growth, as can PLGA for brain 66 and islet β‐like cell 67 organoids. Lancaster et al.…”

Section: Discussionmentioning

confidence: 99%

Engineered extracellular matrices facilitate brain organoids from human pluripotent stem cells

Muñiz,

Topal,

Brooks

et al. 2023

Ann Clin Transl Neurol

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Objective Brain organoids are miniaturized in vitro brain models generated from pluripotent stem cells, which resemble full‐sized brain more closely than conventional two‐dimensional cell cultures. Although brain organoids mimic the human brain's cell‐to‐cell network interactions, they generally fail to faithfully recapitulate cell‐to‐matrix interactions. Here, an engineered framework, called an engineered extracellular matrix (EECM), was developed to provide support and cell‐to‐matrix interactions to developing brain organoids. Methods We generated brain organoids using EECMs comprised of human fibrillar fibronectin supported by a highly porous polymer scaffold. The resultant brain organoids were characterized by immunofluorescence microscopy, transcriptomics, and proteomics of the cerebrospinal fluid (CSF) compartment. Results The interstitial matrix‐mimicking EECM enhanced neurogenesis, glial maturation, and neuronal diversity from human embryonic stem cells versus conventional protein matrix (Matrigel). Additionally, EECMs supported long‐term culture, which promoted large‐volume organoids containing over 250 μL of CSF. Proteomics analysis of the CSF found it superseded previous brain organoids in protein diversity, as indicated by 280 proteins spanning 500 gene ontology pathways shared with adult CSF. Interpretation Engineered EECM matrices represent a major advancement in neural engineering as they have the potential to significantly enhance the structural, cellular, and functional diversity that can be achieved in advanced brain models.

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Section: Discussionmentioning

confidence: 99%

Engineered extracellular matrices facilitate brain organoids from human pluripotent stem cells

Muñiz,

Topal,

Brooks

et al. 2023

Ann Clin Transl Neurol

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show abstract

“…Furthermore, this PLGA-based organoid system was transplanted into a diabetic rat model, demonstrating the potential for type 1 diabetes treatment. [121] Moreover, retinal organoid differentiation was achieved by employing synthetic ECM materials, a vitronectin-mimicking oligopeptide-based scaffold, as a substitute for Matrigel. [122] By using the oligopeptide scaffold, 100% aggregation efficiency was achieved with mouse embryonic stem cells, and the size of the organoid was increased when compared to Matrigel groups.…”

Section: Synthetic Matrices For Organoid Researchmentioning

confidence: 99%

Bioengineering Approaches for the Advanced Organoid Research

Zhang

Rathnam

et al. 2021

Advanced Materials

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organoids should exhibit essential features, including organ-specific multiple cell types, functions of the organ, and spatially organized structures. The emergence and progression of organoid technologies have resulted from several important discoveries (Figure 1). The formation of actual tissuelike colonies in vitro was firstly observed from a co-culture system of keratinocytes and 3T3 fibroblasts. [4] Self-organization, one of the fundamental aspects of organogenesis, was first observed via two distinct approaches, namely reaggregation and structural patterning of dissociated single cells. [5,6] The establishment of 3D culture methods for the structural organization began with the development of extracellular matrices (ECM).In the late 1980s, Bissell and colleagues observed that a laminin-rich gel could function as a basement membrane to differentiate and morphogenesis of mammary epithelial cells. [7,8] In the 1990s, it was reported that in addition to their primary role in physical support, ECM components could regulate gene expression by interacting with integrin-based focal adhesion pathways. [9] Finally, in 2009, Clevers group reported that embedding single intestinal stem cells in ECM substitute had created crypt-like structures similar to the epithelium of the native intestinal tissues, which were the first organoids. [10] Based on these recognitions, biochemical cues that include the initiation of lineage-specific genetic programs have been incorporated in 3D organoid cultures. Through exposure to morphogens, growth factors, or morphogen inhibitors, multiple research groups rapidly developed various organoid models using embryonic stem cells (ESCs) or adult stem cells (ASCs); these include intestine, [10] stomach, [11] liver, [12] pancreas, [13] prostate, [14] and brain [15] organoids. At the same time, vascularization techniques were devised by several groups to embody microenvironments that are physiologically close to their actual counterparts. Microfluidic systems, [16] endothelial cell-coated modules, [17] and vascular endothelial growth factor delivery systems [18] have been demonstrated as in vitro vasculature systems that can facilitate oxygen or nutrients transport to the inner mass of organoids.In the late 2010s, owing to the accumulated information on mechanisms underlying organogenesis and the remarkable advancements in the fields of biomaterial and bioengineering, the era of "organoid customization" has begun. Customizable hydrogel matrices have been proposed to form intestinal organoids whose internal networks recapitulate the Recent advances in 3D cell culture technology have enabled scientists to generate stem cell derived organoids that recapitulate the structural and functional characteristics of native organs. Current organoid technologies have been striding toward identifying the essential factors for controlling the processes involved in organoid development, including physical cues and biochemical signaling. There is a growing demand for engineering dynamic niches characterized by ...

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Advances and challenges of endocrine pancreas bioengineering

Hanna

Berishavili

2022

Current Opinion in Endocrine and Metabolic Research

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Liraglutide Immobilized on Poly(lactic-co-glycolic acid) Polymer Films Induced the Differentiation of Islet β-Like Cells from Bone Marrow Mesenchymal Stem Cells

Cited by 3 publications

References 37 publications

Engineered extracellular matrices facilitate brain organoids from human pluripotent stem cells

Engineered extracellular matrices facilitate brain organoids from human pluripotent stem cells

Bioengineering Approaches for the Advanced Organoid Research

Advances and challenges of endocrine pancreas bioengineering

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