Liquorproteomanalyse bei klinisch isoliertem Syndrom und MS

Tumani, Hayrettin; Rau, Daniela; Lehmensiek, Vera; Guttmann, I.; Tauscher, G.; Palm, Christian; Hirt, V.; Süßmuth, Sigurd D.; Brettschneider, Johannes

doi:10.1007/s00115-009-2777-2

Cited by 2 publications

(2 citation statements)

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“…Several trends (e.g., increase in APOD and ZAG; decrease in NPTX1) corroborated previous reports. − At the same time, there was no consistent change in the levels of prospective multiple sclerosis markers SCG1 and LRG1. , …”

Section: Resultssupporting

confidence: 88%

FastCAT Accelerates Absolute Quantification of Proteins Using Multiple Short Nonpurified Chimeric Standards

et al. 2022

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Absolute (molar) quantification of clinically relevant proteins determines their reference values in liquid and solid biopsies. The FastCAT (for Fast-track QconCAT) method employs multiple short (<50 kDa), stable-isotope labeled chimeric proteins (CPs) composed of concatenated quantotypic (Q)-peptides representing the quantified proteins. Each CP also comprises scrambled sequences of reference (R)-peptides that relate its abundance to a single protein standard (bovine serum albumin, BSA). FastCAT not only alleviates the need to purify CP or use sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) but also improves the accuracy, precision, and dynamic range of the absolute quantification by grouping Q-peptides according to the expected abundance of the target proteins. We benchmarked FastCAT against the reference method of MS Western and tested it in the direct molar quantification of neurological markers in human cerebrospinal fluid at the low ng/mL level.

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Section: Resultssupporting

confidence: 88%

FastCAT Accelerates Absolute Quantification of Proteins Using Multiple Short Nonpurified Chimeric Standards

et al. 2022

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“…increase in APOD and ZAG; decrease in NPTX1) corroborated previous reports. [55][56][57] At the same time, there was no consistent change in the levels of prospective multiple sclerosis markers SCG1 and LRG1. 48,58 Notably, the amount of spiked CP05 standard was ca 10-fold higher than of CP06 (309 fmol/column vs. 33 fmol/column, respectively).…”

Section: Concentrations Determined In Individual Patients (Supplement...mentioning

confidence: 91%

FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards

Rzagalinski

Bogdanova

Raghuraman

et al. 2021

Preprint

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Absolute (molar) quantification of proteins provides the analytical rationale for system-level modelling of diverse molecular mechanisms. FastCAT method employs multiple short (<50 kDa) stable-isotope labeled chimeric proteins (CPs) composed of concatenated quantotypic (Q-) peptides representing the quantified proteins. Each CP also comprises scrambled sequences of reference (R-) peptides that relate its abundance to a single protein standard (BSA). FastCAT not only alleviates the need in purifying CP or using SDS-PAGE, but also improves the accuracy, precision and dynamic range of the absolute quantifications by grouping Q-peptides according to the expected abundance of target proteins. We benchmarked FastCAT against the reference method of MS Western and tested it in the direct molar quantifications of neurological markers in human cerebrospinal fluid at the low ng/mL level.

show abstract

Liquorproteomanalyse bei klinisch isoliertem Syndrom und MS

Cited by 2 publications

References 0 publications

FastCAT Accelerates Absolute Quantification of Proteins Using Multiple Short Nonpurified Chimeric Standards

FastCAT Accelerates Absolute Quantification of Proteins Using Multiple Short Nonpurified Chimeric Standards

FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards

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