Liquordiagnostik

Reiber, Hansotto

doi:10.1007/3-540-31176-9_6

Cited by 2 publications

(1 citation statement)

References 47 publications

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“…Ein zentrales Qualita Ètsmerkmal der Liquordiagnostik, die Auswertung von Liquor/Serum-Konzentrationsquotienten fu Èr Serumproteine [4,5]…”

Section: Qualita èTskontrolleunclassified

Liquordiagnostik-Ausbildung und Fachqualifikation: Richtlinien der Deutschen Gesellschaft für Liquordiagnostik und Klinische Neurochemie (DGLN)/Cerebrospinal Fluid – Diagnosis Education and Certification Guidelines of the German Society for CSF Diagnosis and Clinical Neurochemistry (DGLN)

Reiber¹,

Uhr²

2003

LaboratoriumsMedizin

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Zusammenfassung: Wir berichten u Èber die neuen Richtlinien der Deutschen Gesellschaft fu Èr Liquordiagnostik und klinische Neurochemie (DGLN) zur Erlangung der Fachqualifikation Liquordiagnostik (Liquorzertifikat), der Ausbildungsberechtigung und zur Spezifikation von Ausbildungslabors. Empfehlungen der DGLN zur internen und externen Qualita Ètskontrolle (Ringversuch) werden angesprochen und eine Erweiterung der Richtlinien der Bundesa Èrztekammer bezu Èglich der Einfu Èhrung von Liquor/Serum-Konzentrationsquotienten vorgeschlagen. Die Strukturbedingungen eines Routinelabors fu Èr Liquordiagnostik, wie qualifizierte Zytologie, integrierter Befundbericht und ada Èquate Asservierung der Liquor/Serum-Proben wird diskutiert.Schlu È sselwo È rter: Liquordiagnostik, Ausbildung Summary: The German Society for CSF Diagnosis and Clinical Neurochemistry (DGLN) has developed guidelines for the education and qualified performance of CSF analysis. The guidelines specify the conditions for obtaining a personal certificate or a certificate for a qualified training laboratory. This article refers also to the recommendations of the DGLN regarding internal and external quality control (CSF survey) and a proposal for the extension of the current guidelines of thè Bundesa Èrztekammer' to include CSF/serum concentration quotients. The structural conditions of a routine laboratory for CSF analysis are discussed regarding qualified cytology, integrated CSF data report and careful asservation of the CSF samples.

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“…Ein zentrales Qualita Ètsmerkmal der Liquordiagnostik, die Auswertung von Liquor/Serum-Konzentrationsquotienten fu Èr Serumproteine [4,5]…”

Section: Qualita èTskontrolleunclassified

Liquordiagnostik-Ausbildung und Fachqualifikation: Richtlinien der Deutschen Gesellschaft für Liquordiagnostik und Klinische Neurochemie (DGLN)/Cerebrospinal Fluid – Diagnosis Education and Certification Guidelines of the German Society for CSF Diagnosis and Clinical Neurochemistry (DGLN)

Reiber¹,

Uhr²

2003

LaboratoriumsMedizin

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Klotho and fibroblast growth factor 23 in cerebrospinal fluid in children

et al. 2016

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The fibroblast growth factor (FGF) 23/Klotho axis is a principal regulator of phosphate hemostasis and vitamin D metabolism, but limited data is available on its role in the central nervous system. Here, we investigate soluble α-Klotho (sKlotho) and C-terminal as well as intact FGF23 in cerebrospinal fluid (CSF) and plasma and their relationship to mineral metabolism parameters in humans. In 39 children aged 0.3-16.8 years undergoing lumbar puncture for the exclusion of inflammatory neurological disease, sKlotho and FGF23 were investigated by Western blot analysis, followed by ELISA quantification in CSF and plasma. The percentage of intrathecal synthesis of both proteins was calculated by measuring both the expected and observed CSF/plasma ratios of sKlotho and FGF23. The secreted (KL1) and cleaved (KL1+KL2) isoforms of sKlotho, and FGF23 were clearly detected in CSF in all subjects, although protein levels were lower compared to those of plasma samples (each p < 0.01). The intrathecal percentage of CSF sKlotho and FGF23 synthesis amounted to 98 and 99 %, respectively. CSF sKlotho levels were higher in boys than in girls (p < 0.01), and correlated positively with plasma C-terminal FGF23 concentrations (p < 0.05) and standardized height (p < 0.01). Importantly, there were no significant correlations between plasma and CSF levels of sKlotho or FGF23. Plasma sKlotho as well as C-terminal and intact FGF23, respectively, were associated with parameters of mineral metabolism These results provide evidence that cleaved and secreted sKlotho and FGF23 are present in CSF, mainly derived from brain and affected by sex, height, and mineral metabolism parameters in children. Nevertheless, the absence of significant associations between plasma and CSF levels of Klotho and FGF23, respectively, suggest that the regulation of Klotho and FGF23 may be different between organs secreting these hormones into blood and CSF.

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Liquordiagnostik

Cited by 2 publications

References 47 publications

Liquordiagnostik-Ausbildung und Fachqualifikation: Richtlinien der Deutschen Gesellschaft für Liquordiagnostik und Klinische Neurochemie (DGLN)/Cerebrospinal Fluid – Diagnosis Education and Certification Guidelines of the German Society for CSF Diagnosis and Clinical Neurochemistry (DGLN)

Liquordiagnostik-Ausbildung und Fachqualifikation: Richtlinien der Deutschen Gesellschaft für Liquordiagnostik und Klinische Neurochemie (DGLN)/Cerebrospinal Fluid – Diagnosis Education and Certification Guidelines of the German Society for CSF Diagnosis and Clinical Neurochemistry (DGLN)

Klotho and fibroblast growth factor 23 in cerebrospinal fluid in children

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