“…The solubilized sample was added to the column and eluted with PE (450 mL), followed by DCM (350 mL), and finally with MeOH (500 mL), thus yielding the PE fraction, dichloromethane fraction, and methanolic fraction. The fractions obtained were dried in an extraction hood for 24 hours (Mancarz et al, 2019;Yang et al, 2011).…”
Section: Preparation Of the Liquid-liquid Partitions Of Propolismentioning
Background: Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer to understanding the response generated by the complex microbial ecosystems of the oral cavity. The objective of this study was to evaluate the antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro biofilm of Streptococcus gordonii and Fusobacterium nucleatum. Methods: The experimental work involved a consecutive, in vitro, longitudinal, and double-blinded study design. Propolis samples were collected from 13 different regions of the Peruvian Andes. The disk diffusion method was used for the antimicrobial susceptibility test. The cytotoxic effect of propolis on human gingival fibroblasts was determined by cell viability method using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and the effect of propolis on the biofilm was evaluated by confocal microscopy and polymerase chain reaction (PCR). Results: The 0.78 mg/mL and 1.563 mg/mL concentrations of the methanolic fraction of the chloroform residue of Oxapampa propolis showed effects on biofilm thickness and the copy numbers of the srtA gene of S. gordonii and the radD gene of F. nucleatum at 48 and 120 hours, and chromatography (UV, λ 280 nm) identified rhamnocitrin, isorhamnetin, apigenin, kaempferol, diosmetin, acacetin, glycerol, and chrysoeriol. Conclusions: Of the 13 propolis evaluated, it was found that only the methanolic fraction of Oxapampa propolis showed antibacterial and antibiofilm effects without causing damage to human gingival fibroblasts. Likewise, when evaluating the chemical composition of this fraction, eight flavonoids were identified.
“…The solubilized sample was added to the column and eluted with PE (450 mL), followed by DCM (350 mL), and finally with MeOH (500 mL), thus yielding the PE fraction, dichloromethane fraction, and methanolic fraction. The fractions obtained were dried in an extraction hood for 24 hours (Mancarz et al, 2019;Yang et al, 2011).…”
Section: Preparation Of the Liquid-liquid Partitions Of Propolismentioning
Background: Natural products with antibacterial potential have begun to be tested on biofilm models, bringing us closer to understanding the response generated by the complex microbial ecosystems of the oral cavity. The objective of this study was to evaluate the antibacterial, antibiofilm, and cytotoxic activities and chemical compositions of Peruvian propolis in an in vitro biofilm of Streptococcus gordonii and Fusobacterium nucleatum. Methods: The experimental work involved a consecutive, in vitro, longitudinal, and double-blinded study design. Propolis samples were collected from 13 different regions of the Peruvian Andes. The disk diffusion method was used for the antimicrobial susceptibility test. The cytotoxic effect of propolis on human gingival fibroblasts was determined by cell viability method using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and the effect of propolis on the biofilm was evaluated by confocal microscopy and polymerase chain reaction (PCR). Results: The 0.78 mg/mL and 1.563 mg/mL concentrations of the methanolic fraction of the chloroform residue of Oxapampa propolis showed effects on biofilm thickness and the copy numbers of the srtA gene of S. gordonii and the radD gene of F. nucleatum at 48 and 120 hours, and chromatography (UV, λ 280 nm) identified rhamnocitrin, isorhamnetin, apigenin, kaempferol, diosmetin, acacetin, glycerol, and chrysoeriol. Conclusions: Of the 13 propolis evaluated, it was found that only the methanolic fraction of Oxapampa propolis showed antibacterial and antibiofilm effects without causing damage to human gingival fibroblasts. Likewise, when evaluating the chemical composition of this fraction, eight flavonoids were identified.
“…Although there are antioxidant and antimicrobial studies on strains Liquidambar styraciflua L [36][37][38] . ve Liquidambar formosana [39][40][41] , there are few reports on Liquidambar orientalis, an endemic species that we investigated.…”
Section: Staphylococcus Aureus (S Aureus) Atcc 6538pmentioning
In the study, sweetgum tree (Liquidambar orientalis), which is an endemic species that grows in Mugla, Köyceğiz and is applied for medicinal purposes among the public, its leaves was examined. The antioxidant ability of the extract obtained from dried plant leaves has been evaluated using a variety of methods which are Total Phenolic Substance, Total Flavonoid, FRAP, CUPRAC, DPPH, and ABTS + . Simultaneously, the antimicrobial activity of the plant extract was examined using disk diffusion and microdilution methods to determine the minimum inhibitor concentration (MIC). While the total phenolic content of Liquidambar orientalis extract was 96.34 mg GAE/g, the total amount of flavonoid was 2.15 mg QE/g. When the results of the antioxidant analysis were examined, it was observed that it had a good level of antioxidant activity with the results of 49.25 ± 0.54 mmol TEAC/g according to the CUPRAC method, 39.83 ± 0.25 µmol Fe/g according to the FRAP method, 80.34 μg/mL according to the DPPH method and 51.20 μg/mL according to the ABTS+ method. As a result of the antimicrobial analysis, it was indicated that L. orientalis extract was more effective on Staphylococcus aureus (S. aureus), which is a gram-positive bacterium and causes a wide variety of clinical diseases. Even, L. orientalis extract with an MIC value of 10 mg/mL has been found to have a higher antibacterial effect than Amoxicillin+Clavulanic acid, which is used as a standard drug in that field. This research is significant because it is the first to report the determination of all biological activities for L. orientalis, including total polyphenols, flavonoid contents, antioxidant content, and antimicrobial activity.
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