Liquid triphasic systems as sustainable downstream processing of Chlorella sp. biorefinery for potential biofuels and feed production

Koyande, Apurav Krishna; Chew, Kit Wayne; Show, Pau Loke; Munawaroh, Heli Siti Halimatul; Chang, Jo Shu

doi:10.1016/j.biortech.2021.125075

Cited by 24 publications

(22 citation statements)

References 45 publications

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“…In addition, the investigated TPP systems also showed improvements in relation to traditional TPP systems. Diverse studies 40,[72][73][74] reported that the optimized concentration of salt in butanol/(NH 4 ) 2 SO 4 TPP systems ranged from 30 to 70% w/w, while the amount of salt required in our investigation ranged from 10 to 25%w/w.…”

Section: Performance Comparisonmentioning

confidence: 73%

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Antunes,

Temmink,

Schuur

2023

J of Chemical Tech & Biotech

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BackgroundNatural polymers are macromolecules produced by living organisms, with a wide range of applications and relevance for the development of a circular economy. However, large‐scale production is still hindered by several factors, such as downstream processing. In this work, three‐phase partitioning (TPP) systems were investigated for separation of model polysaccharide (dextran, alginate and gum arabic) from protein (Bovine serum albumin (BSA) and lysozyme). The recyclability of phase‐forming compounds used to form the extractive platform was assessed by ultrafiltration (UF). This study contributes to development of production processes for biopolymers from fermented waste by proposing an effective separation technique for fractionation of biopolymers. Previously such biopolymers were only collected as mixtures, and with the studied approaches, also fractionation may be applied of polysaccharides from proteins. With the chosen systems, the scope of TPP systems is expanded by using another class of phase‐forming compound (polymers), and in addition, UF was studied as a versatile regeneration approach.ResultsWithin the TPP approach, the best separation of dextran from BSA was achieved using TPP systems composed of 25 wt% PEG + 25 wt% K3C6H5O7 and 36 wt% EtOH + 10 wt% K3PO4, in which more than 95% of dextran and BSA were found as precipitate and partitioned to top phase (PEG or EtOH‐rich), respectively. By using other model compounds, it was found that the molecular weight and charge of biopolymer play a key role in the yield and selectivity of TPP systems. Finally, by using ultrafiltration/diafiltration, about 99% of ethanol and phosphate salt used to form the extractive platform could be retrieved in the permeate stream.ConclusionThe high extraction yields, selectivity and recyclability of phase‐forming compounds confirm the potential of polymer‐based and alcohol‐based TPP systems to fractionate biopolymer mixtures.This article is protected by copyright. All rights reserved.

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Section: Performance Comparisonmentioning

confidence: 73%

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Antunes,

Temmink,

Schuur

2023

J of Chemical Tech & Biotech

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“…Ammonium sulfate was selected as the preferred salt for the subsequent experiments as the PR%, SE%, CR%, and CHL R% was highest with this salt. Several other studies also reported AS as the preferred phase‐forming material in three‐phase portioning 12,42,58 …”

Section: Resultsmentioning

confidence: 83%

“…Some studies have reported 40% (w/v) of AS concentration to be optimum for protein accumulation, like in this study. 12,30,60,61 In other studies, 50% (w/v) of AS concentration was reported to be the optimum for protein yield. 41,53 However, Chew et al found a lower value of AS concentration (30% w/v) to be optimum for protein recovery yield from the biomass of Chlorella vulgaris in a study of microwave-assisted threephase partitioning.…”

Section: Results and Discussion Effect Of Salt Typementioning

confidence: 84%

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Simultaneous extraction of chlorophylls, proteins, and carbohydrates from isolated Chlorella thermophila using a triphasic separation technique: A biorefinery approach

Sarkar

Bhowmick

Gayen

2023

Biofuels Bioprod Bioref

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The separation of multiple products from microalgae using a biorefinery approach is essential for the economic sustainability of microalgae-based products. In the current study, a technique for the simultaneous extraction of such products was investigated for the complete valorization of microalgae biomass. Three principal metabolites -chlorophylls, carbohydrates and proteins -were simultaneously fractionated from the biomass of isolated Chlorella thermophila using a modified version of the triphasic separation (TPS) process. Optimized process parameters were determined as: (i) ammonium sulfate as the salt; (ii) 40% (w/v) as the salt concentration; (iii) 1:1 as the salt/alcohol ratio; (iv) 0.25% (w/w) as biomass loading; (v) 6 min of homogenization time, and (vi) 10 000 rpm as homogenization speed. These optimized process parameters resulted in the simultaneous recovery of 71%, 40%, and 22% yields for proteins, carbohydrates, and chlorophylls, respectively. Ultrastructure analysis revealed that intracellular metabolites could be extracted without complete cell disruption. Finally, a comparative analysis between triphasic separation with and without homogenization demonstrated the economic feasibility of the optimized extraction process.

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“…Algal biomass contains typically three essential components: 30–40% protein, 20–40% carbohydrate, and 15–20% lipid based on its dry weight. In the algal biorefinery process, the algal biomass is cultivated using wastewater and processed for different biofuels and value-added products through different conversion platforms . Notably, there are three major algal conversion platforms: (1) biochemical conversion results in biofuels, bioalcohols, and bioplastics, (2) chemical conversion results in biodiesel, and (3) thermochemical conversion results in syngas, biochar, biocrude, or bio-oil.…”

Section: Algal Biorefinerymentioning

confidence: 99%

A Mini Review of Biochemical Conversion of Algal Biorefinery

et al. 2021

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Algal biomass is rich in essential components such as proteins, carbohydrates, and lipids, which are considered as a potential feedstock for algaebased fuel and chemical production. The commercial application of algae-based fuel production is a challenging task because algal cultivation and harvesting demand high energy and cost. It is a common bottleneck problem in algae processed for fuel or chemical production at the pilot scale. The execution of the concept of integrated algal biorefinery has the possibility to reduce the demand for energy and costs. It is comparatively economical to conventional methods in terms of the recovery of various value-added bioproducts including biopolymers, biochemicals, biofertilizers, and biofuels. In addition, this approach will decrease the overall production costs and aid in making an algal-based green economy viable. This mini review aims to provide certain information regarding development of and updates on cost-effective integrated biorefinery of algal-based bioproducts recovery and its potential application. In connection to this, life cycle assessment and techno-economic aspects of integrated algal biorefinery are discussed. Finally, present challenges and future research directions for integrated algal biorefinery are outlined.

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Liquid triphasic systems as sustainable downstream processing of Chlorella sp. biorefinery for potential biofuels and feed production

Cited by 24 publications

References 45 publications

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Polymer and alcohol‐based three‐phase partitioning systems for separation of polysaccharide and protein

Simultaneous extraction of chlorophylls, proteins, and carbohydrates from isolated Chlorella thermophila using a triphasic separation technique: A biorefinery approach

A Mini Review of Biochemical Conversion of Algal Biorefinery

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