Liquid Scintillation Counting of Tritiated Organic Compounds

Abstract

The use of tritium-tagged hydrocarbons in analytical research has made it necessary to evaluate some of the parameters involved in liquid scintillation-counting techniques. From these evaluations, a routine procedure for measuring the radioactivity of tritiated organic compounds and the confidence limits of results were established. This paper evaluates the more significant variables and sets forth the conditions and procedure adopted by this laboratory.

Transport of p‐Aminohippuric Acid (3H‐PAH), Inulin and Dextran Out of the Cranial Cavity: A Methodological Study Using Intraventricular Injection and Sample Combustion

“…of I4C, respectively. These amounts of activity added to the sample count exceeded the sample count by more than 1O:l. This relationship gives good statistical accuracy in the calculated corrected count (Herberg 1963;Whisman e/ al. 1960) at a very low cost.…”

Preparation of Tritium-Labeled 1-Hexene and 1-Octene

“…of the tritium, giving a specific activity of 150 me. per gram, as assayed by liquid scintillation counting (7). A portion of the exposed material then was processed by gas-liquid chromatography to separate the 1-hexene that had been produced.…”

Radiometric techniques for the detection and assessment of tritium in aqueous media - a review

“…Within 5 years, LSC techniques had become the primary means of analysing liquid samples for 3 H content and, by 1962, counting efficiencies of 25% had been reported for HTO [35]. The technique was demonstrated to be used effectively for the analysis of 3 H in water [39], urine [39], blood and other bodily tissues [40], organic matter [41], as well as environmental water samples. By 1962,…”