Liquid scintillation counting of tritiated water in plasma following dioxane precipitation

Springell, PH; Wright, D. E.

doi:10.1016/0020-708x(76)90181-2

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…The measurement of total body water and water turnover based on the tritiated water dilution technique described by and modified by Springell & Wright (1976) was employed and well validated in the present study. TOH space was least for Maradi goats, 661 % LW, while the value for Sahel goats was 77-8 % LW.…”

Section: Discussionmentioning

confidence: 99%

Breed differences in water metabolism and body composition of sheep and goats

et al. 1989

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SUMMARYWater metabolism of two breeds of sheep (Uda and Yankasa)and two breeds of goat (Sahel and Maradi) was studied. Tritiated water space as a percentage of live weight was 80·5 and 73·6 % for Uda and Yankasa, respectively, and 77·8 and 66·1 % for Sahel and Maradi, respectively. The biological half-life of tritium was least in Yankasa (115 h) and longest in Sahel (173 h). Water turnover (I/day per animal) was 2·52, 2·63, 0·86 and 1·09 for Uda, Yankasa, Sahel and Maradi, respectively.

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Section: Discussionmentioning

confidence: 99%

Breed differences in water metabolism and body composition of sheep and goats

et al. 1989

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“…Blood samples were obtained before and 2 h after each isotope injection and at 48 h intervals for the 7 days between isotope injections. Water was recovered from the blood samples following dioxane precipitation (Springell and Wright 1976) and radioactivity was counted as described by Martin, McMeniman and Dowsett (1991). Mean daily water intakes were estimated for the foals after accounting for their increase in body weight and tritiated water space during that period.…”

Section: Milk Intakementioning

confidence: 99%

Milk and water intakes of foals sucking grazing mares

Martin

McMeniman

Dowsett

1992

Equine Veterinary Journal

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Summary Intakes of milk and milk nutrients were determined for 8 foals at 11–18 days of age and for 10 foals at 30–44 days and 60–74 days of age while sucking grazing mares. Water intakes (sources other than milk) of the foals were determined at 30–44 days and 60–74 days of age. Five of the 10 mares were fed a protein supplement (24% crude protein) in addition to grazing during the stud season. The protein supplement did not influence foal intakes of milk and milk nutrients, milk composition, weight gains of the mares or the growth rate of the foals. Foal milk intakes increased (P<0.05) from 16.9 kg/day at 11–18 days to 18.1 kg/day at 60–74 days of age. The water intakes of the foals increased (P<0.01) from 3.9 kg/day at 30–44 days to 5.5 kg/day at 60–74 days of age. Total fluid intakes per kg foal liveweight were 246, 202 and 172 g at 11–18, 30–44 and 60–74 days of age, respectively. For each kg of weight gain, foals consumed 12.8, 15.7 and 16.4 kg milk at 11–18, 30–44 and 60–74 days of age. Stage of lactation had a significant effect on the total solids, lactose and protein content of milk. The fat and gross energy content of milk remained constant.

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“…At the beginning of each period, lambs were injected intramuscularly with a 200 !lCi dose of tritiated water (I ml) in saline solution (Macfarlane et al 1969) and plasma samples were measured for activity by liquid scintillation (Springell & Wright 1976). …”

Section: Lamb Water Turnovermentioning

confidence: 99%

Lactation performance, growth, and carcass composition of sheep

Geenty¹,

Clarke

Wright³

1985

New Zealand Journal of Agricultural Research

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In 2 experiments ewe milk production, lamb water turnover, and lamb growth were measured during early lactation in Romney, Dorset, and crossbred sheep at pasture. Water turnover began to exceed milk production during Weeks 2-4 of lactation in single lambs reared by crossbred and Dorset ewes and in twins reared by Dorsets. In contrast, lambs suckling Romney ewes appeared to face a milk deficit during the first 3 weeks of lactation, particularly twins, when up to 34% of their food intake was from herbage. Despite large differences between ewe breeds in milk production, differences in lamb water turnover and growth rate were small. For ewes rearing twin lambs, Dorsets produced 58% more milk than Romneys but their lambs had only 19% greater average water turnover and were 15% heavier at 3 1 /2 weeks-of-age. Within breeds, rearing status markedly affected milk production but not water turnover. Water turnover was more closely related to final lamb liveweight for single lambs (r2 = 0.35 to 0.74) than was milk production (r2 = 0.34 to 0.62). Logarithmic regressions (pooled within breeds) showed a close association between lamb body water space and liveweight (r 2 = 0.72 to 0.96). Breed relationships between ewe milk production and the consumption of milk and herbage by lambs are discussed in relation to the comparatively small differences among breeds in final lamb liveweight.

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Liquid scintillation counting of tritiated water in plasma following dioxane precipitation

Cited by 7 publications

References 9 publications

Breed differences in water metabolism and body composition of sheep and goats

Breed differences in water metabolism and body composition of sheep and goats

Milk and water intakes of foals sucking grazing mares

Lactation performance, growth, and carcass composition of sheep

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