Liquid scintillation counting of paper chromatograms

Wang, C.H.; Jones, Dayton

doi:10.1016/0006-291x(59)90020-8

Cited by 124 publications

(9 citation statements)

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“…The solvents were n-butanol : acetic acid: water (4:1:1 v/v); n-butanol saturated with 5% (w/v) aqueous trichloroacetic acid; and phenol:0.01 N hydrochloric acid (4:1 w/v) saturated with S02. The distribution of radioactivity on the papers was measured in a liquid scintillation spectrometer by the method of Wang & Jones (1959). In each case a single major peak corresponding in RF to authentic adrenaline or noradrenaline accounted for 95 to 99% of the radioactivity on the chromatograms.…”

Section: Methodsmentioning

confidence: 99%

Retention of Injected Catechol Amines by the Mouse

Iversen

Whitby

1962

British Journal of Pharmacology and Chemotherapy

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The importance of tissue storage as a method of inactivation of circulating catechol amines has been assessed by measuring the amount of unchanged hormone remaining in the mouse 30 min after the injection of various doses of [3H]‐adrenaline or [3H]‐noradrenaline. The results show that this method of inactivation is quantitatively more important for noradrenaline than for adrenaline at all dose levels studied, and that for both hormones storage is relatively more important at physiological dose levels (3 to 30 μg/kg) than at higher dose levels (150 to 300 μg/kg). The results obtained after the simultaneous injection of various doses of [3H]‐adrenaline and [14C]‐noradrenaline show that under certain conditions the two hormones compete for entry into the tissue storage sites. The possible nature of the mechanisms by which circulating catechol amines enter the tissue stores is discussed in the light of previous findings on the uptake of catechol amines by tissues in vitro.

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Section: Methodsmentioning

confidence: 99%

Retention of Injected Catechol Amines by the Mouse

Iversen

Whitby

1962

British Journal of Pharmacology and Chemotherapy

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“…1 filter paper. The chromatograms were developed with an n-butanol: acetic acid: water (4:1: 1 v/v) mixture as a descending system, and the distribution of radioactivity on the papers was measured in a liquid scintillation spectrometer by the method of Wang & Jones (1959). that Fraction I yielded a single peak of radioactivity corresponding in RF to authentic noradrenaline, and Fraction II yielded a smaller peak corresponding in R F to authentic normetanephrine together with a minor peak of noradrenaline.…”

Section: Perfusionsmentioning

confidence: 99%

The Uptake of Noradrenaline by the Isolated Perfused Rat Heart

Iversen

1963

British Journal of Pharmacology and Chemotherapy

240

172

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The uptake of noradrenaline by the isolated perfused rat heart was studied after perfusion with a medium containing various concentrations of (+)‐[3H]‐noradrenaline. Simultaneous measurement of the uptake of [3H]‐noradrenaline and of the net increase in the noradrenaline content of the heart showed that [3H]‐noradrenaline entering the heart both increased the tissue content and exchanged with endogenous noradrenaline. A large part (about 75%) of the endogenous noradrenaline pool, however, exchanged very slowly if at all with exogenous noradrenaline. The initial rates of noradrenaline uptake satisfied Michaelis‐Menten kinetics with a Km for (±)‐noradrenaline of 6.64times10−7 μM. Further analysis of the uptake process indicated that noradrenaline entered into at least two intracellular pools at different rates. Measurement of the initial rates of noradrenaline uptake during perfusion with various concentrations of nonradioactive (+)‐ and (−)‐noradrenaline showed that the uptake process exhibited stereochemical specificity. The Km values for (+)‐ and (−)‐noradrenaline were 13.9times10−7 and 2.66times10−7 μM respectively. Cocaine acted as a potent competitive inhibitor of noradrenaline uptake. This finding suggested that diffusion did not play any significant role in the entry of noradrenaline into the tissue.

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“…Le volume de la réaction est de 10 ou 15 pl, la concentration en substrat d'au moins 0,0015 M; après addition de 10 ou 15 pl d'alcool absolu, les produits sont séparés par chromatographie sur papier (butanol sec: acide formique 85%: eau, 75:15:10) [17] sur une distance de 20 cm. La répartition de la radio activité est déterminée par comptage dans un scintillatene liquide [56].…”

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Leucine aminopeptidase et naphtylamidases dans l’épiderme humain

Bersaques¹

1968

Enzymol Biol Clin

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Liquid scintillation counting of paper chromatograms

Cited by 124 publications

References 1 publication

Retention of Injected Catechol Amines by the Mouse

Retention of Injected Catechol Amines by the Mouse

The Uptake of Noradrenaline by the Isolated Perfused Rat Heart

Leucine aminopeptidase et naphtylamidases dans l’épiderme humain

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