Liquid scanning transmission electron microscopy: Nanoscale imaging in micrometers-thick liquids

Abstract

Microscopie électronique à balayage en transmission en phase liquide : Imager à l'échelle du nanomètre à travers des films liquides de plusieurs micromètres d'épaisseur

In situ liquid-cell transmission electron microscopy for direct observation of concentration-dependent growth and dissolution of silver nanoparticles

“…32 The assembled liquid-cell stage was loaded into the TEM (JEM-2010F, JEOL, Japan), which was operated at 200 kV. The resistivity of the DI water was 18.2 MU cm, and two SiN x membranes were used to encapsulate the liquid during imaging.…”

Membrane protein stoichiometry studied in intact mammalian cells

“…The spatial resolution achieved is remarkably high, and is due to the Z contrast of STEM Demers et al, 2010;Schuh & de Jonge, 2014). It would not be possible to achieve nanoscale resolution at these thicknesses with transmission electron microscopy.…”

In‐situ Transmission Electron Microscope Techniques for Heterogeneous Catalysis

“…Thus, real‐time atomic‐scale observation of Pt nanoparticle growth in colloidal solutions was realized . Based on theoretical studies, the STEM may have better performance in resolution than the TEM, especially for thick water layers . The post‐specimen lens is not needed for STEM when imaging, as a result, the chromatic blurring caused by energy loss is avoided .…”

“…[114] Based on theoretical studies, the STEM may have better performance in resolution than the TEM, especially for thick water layers. [116,137] The post-specimen lens is not needed for STEM when imaging, as a result, the chromatic blurring caused by energy loss is avoided. [138,139] Meanwhile, the contrast and resolution can be increased by zero-loss filtering since inelastic electrons could be removed.…”